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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med    2011, Vol. 5 Issue (1) : 94-100    https://doi.org/10.1007/s11684-011-0116-z
RESEARCH ARTICLE
Transplantation of placenta-derived mesenchymal stem cells in type 2 diabetes: a pilot study
Ranhua JIANG1, Zhibo HAN2, Guangsheng ZHUO3, Xiaodan QU1, Xue LI1, Xin WANG3, Yuankang SHAO3, Shimin YANG1, Zhong Chao HAN2()
1. Liaoyang Diabetic Hospital, Liaoyang 111000, China; 2. National Engineering Research Center of Stem Cells, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union of Medical College, Tianjin 300020, China; 3. Beijing Health-Biotech. Co., Ltd., Beijing 100176, China
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Abstract

Mesenchymal stem cells (MSC) have been used in clinical trials for severe diabetes, a chronic disease with high morbidity and mortality. Bone marrow is the traditional source of human MSC, but human term placenta appears to be an alternative and more readily available source. Here, the therapeutic effect of human placenta-derived MSC (PD-MSC) was studied in type 2 diabetes patients with longer duration, islet cell dysfunction, high insulin doses as well as poor glycemic control in order to evaluate the safety, efficacy and feasibility of PD-MSC treatment in type 2 diabetes (T2D). Ten patients with T2D received three intravenous infusions of PDSC, with one month interval of infusion. The total number of PDSC for each patient was (1.22–1.51) × 106/kg, with an average of 1.35 × 106/kg. All of the patients were followed up after therapy for at least 3 months. A daily mean dose of insulin used in 10 patients was decreased from 63.7?±?18.7 to 34.7?±?13.4 IU (P<0.01), and the C-peptide level was increased from 4.1?±?3.7 ng/mL to 5.6?±?3.8 ng/mL (P<0.05) respectively after therapy. In 4 of 10 responders their insulin doses reduced more than 50% after infusion. The mean levels of insulin and C-peptide at each time point in a total of 10 patients was higher after treatment (P<0.05). No fever, chills, liver damage and other side effects were reported. The renal function and cardiac function were improved after infusion. The results obtained from this pilot clinical trial indicate that transplantation of PD-MSC represents a simple, safe and effective therapeutic approach for T2D patients with islet cell dysfunction. Further large-scale, randomized and well-controlled clinical studies will be required to substantiate these observations.

Keywords placenta stem cells      treatment of type 2 diabetes     
Corresponding Author(s): HAN Zhong Chao,Email:tihzchan@public.tpt.tj.cn   
Issue Date: 05 March 2011
 Cite this article:   
Ranhua JIANG,Zhibo HAN,Guangsheng ZHUO, et al. Transplantation of placenta-derived mesenchymal stem cells in type 2 diabetes: a pilot study[J]. Front Med, 2011, 5(1): 94-100.
 URL:  
https://academic.hep.com.cn/fmd/EN/10.1007/s11684-011-0116-z
https://academic.hep.com.cn/fmd/EN/Y2011/V5/I1/94
Fig.1  Immunophenotype of placenta derived mesenchymal stem cells (PD-MSC). The PD-MSC were isolated from tissues of human placenta, cultured and expanded in GMP Laboratory for 5 passages, and analyzed by FACS.
Patient12345678910mean±SD
Pre-treatment4860387676906658873863.7±18.7
6 months after treatment2036185446503632391634.7±13.4*
Tab.1  Insulin dosage (IU) before and after treatment
Patient12345678910mean±SD
Pre-treatment8.19.58.99.18.913.613.68.211.07.59.8±2.2
6 months after treatment6.56.14.66.06.07.57.86.39.16.96.7±1.2*
Tab.2  Changes in glycosylated hemoglobin (%) before and after treatment
Time (min)03060120mean±SD
Pre-treatment7.6±4.812.3±6.218.2±11.424.7±20.116.6±13.5
6 months after treatment11.1±5.1*17.7±7.4*24.6±12.2*31.0±19.8*21.6±13.7*
Tab.3  Levels of insulin (μIU/mL) before and after treatment ( = 10)
Time (min)03060120mean±SD
Pre-treatment2.6±2.13.3±2.44.5±3.05.6±4.04.1±3.7
6 months after treatment3.5±2.3*5.3±3.3*6.4±3.3*8.2±3.9*5.6±3.8*
Tab.4  Levels of C-peptide (ng/mL) before and after treatment ( = 10)
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