Effects of resistin on insulin signaling in endothelial cells

doi:10.1007/s11684-009-0029-2

Front Med Chin

2009, Vol. 3

Issue (2) : 136-140 https://doi.org/10.1007/s11684-009-0029-2

RESEARCH ARTICLE

Effects of resistin on insulin signaling in endothelial cells

Zhizhen LI¹, Fangping LI², Jianhong YE², Li YAN², Zuzhi FU²(

)

1. Department of Endocrinology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China; 2. Department of Endocrinology, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China

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Abstract

The objective of this study was to investigate the effects of resistin on insulin signaling in human umbilical vein endothelial cells (HUVECs). HUVECs were incubated with recombinant human resistin (0-100 ng/mL) for 24 h. Akt and endothelial nitric oxide synthase (eNOS) phosphorylation levels of endothelial cells under basal or insulin stimulated conditions were measured by Western blot. Nitric oxide (NO) production of HUVECs was also detected. The results showed that resistin could significantly inhibit Akt and eNOS phosphorylation and NO production in endothelial cells under insulin stimulated conditions (P< 0.05 vs control). But under basal conditions, treatment with resistin could result in a decrease in eNOS phosphorylation (P< 0.05 vs control) but had no effect on NO production and Akt phosphorylation levels. These findings suggested that resistin exerted an inhibitory effect on NO production by inhibiting insulin signaling and eNOS phosphorylation in endothelial cells.

Keywords resistin endothelium nitric oxide endothelial nitric oxide synthase Akt-binding protein, mouse

Corresponding Author(s): FU Zuzhi,Email:lzz940537@163.com

Issue Date: 05 June 2009

Cite this article:

Zhizhen LI,Fangping LI,Jianhong YE, et al. Effects of resistin on insulin signaling in endothelial cells[J]. Front Med Chin, 2009, 3(2): 136-140.

URL:

https://academic.hep.com.cn/fmd/EN/10.1007/s11684-009-0029-2
https://academic.hep.com.cn/fmd/EN/Y2009/V3/I2/136

Tab.1 NO production levels of each group

Fig.1 Akt phosphorylation level in human umbilical vein endothelial cells without insulin stimulation: a representative Western blot of Akt phosphorylation. 1: control group; 2: res15 group; 3: res50 group; 4: res100 group.

Fig.2 Insulin stimulated Akt phosphorylation level in human umbilical vein endothelial cells: a representative Western blot of Akt phosphorylation. 1: control group; 2: res15 group; 3: res50 group; 4: res100 group.

Fig.3 eNOS phosphorylation level in human umbilical vein endothelial cells without insulin stimulation: a representative Western blot of eNOS phosphorylation. 1: control group; 2: res15 group; 3: res50 group; 4: res100 group.

Fig.4 Insulin stimulated eNOS phosphorylation level in human umbilical vein endothelial cells: a representative Western blot of eNOS phosphorylation. 1: control group; 2: res15 group; 3: res50 group; 4: res100 group.

Tab.2 Akt and eNOS phosphorylation levels of each group

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