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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med Chin    2009, Vol. 3 Issue (2) : 187-190    https://doi.org/10.1007/s11684-009-0033-6
RESEARCH ARTICLE
Tim-3 mRNA expression in peripheral blood lymphocytes from asthmatic patients
Xiaoxia LU1, Weikun HU2, Shengdao XIONG1(), Guopeng XU1, Fen LAN1
1. Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 2. Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
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Abstract

The study aimed to detect the expression of the Th1-specific cell surface protein T cell Ig and mucin domain-containing molecule-3 (Tim-3) mRNA in peripheral blood lymphocytes isolated from asthmatic patients and to examine the correlation among Tim-3 mRNA, interleukin-4 (IL-4), interferon-γ (IFN-γ) level, and FEV1/FVC (force expiratory volume in one second/forced vital capacity) to explore the role of Tim-3 in the development and progression of asthmatic inflammation. Tim-3 mRNA expression was detected by reverse transcription polymerase chain reaction (RT-PCR). The IL-4 and IFN-γ levels were determined by using enzyme-linked immunosorbent assay (ELISA). The correlation among Tim-3 mRNA, IL-4, IFN-γ level, and pulmonary ventilatory capacity was analyzed. The expression of Tim-3 mRNA in patients with acute asthma exacerbation was 0.39±0.06, significantly higher than that in patients at the remission stage and controls (0.18±0.05 and 0.07±0.03, P<0.05). The level of IL-4 in patients with acute asthma exacerbation was 68.42±10.54, significantly higher than that in the patients at the remission stage and controls (41.83±9.37 and 32.75±8.16, P<0.05). The level of IL-4 in patients in remission was significantly higher than that in controls (P<0.05). The level of IFN-γ in patients with acute asthma exacerbation was 65.74±7.85, significantly lower than that in patients in remission and the control group (120.84±11.62 and 139.65±13.47, P<0.05). The level of IFN-γ in patients in asthma remission was significantly lower than that in controls (P<0.05). Tim-3 mRNA expression was positively correlated with the level of IL-4 (r=0.68, P<0.05) and negatively with the level of IFN-γ and pulmonary ventilatory capacity (r=-0.85, r=-0.76, both P<0.01). The increased expression of Tim-3 mRNA in peripheral blood lymphocytes might be involved in the development and progression of asthmatic inflammation.

Keywords asthma      airway inflammation      peripheral blood lymphocyte      Tim-3     
Corresponding Author(s): XIONG Shengdao,Email:sdxiong@tjh.tjmu.edu.cn   
Issue Date: 05 June 2009
 Cite this article:   
Xiaoxia LU,Weikun HU,Shengdao XIONG, et al. Tim-3 mRNA expression in peripheral blood lymphocytes from asthmatic patients[J]. Front Med Chin, 2009, 3(2): 187-190.
 URL:  
https://academic.hep.com.cn/fmd/EN/10.1007/s11684-009-0033-6
https://academic.hep.com.cn/fmd/EN/Y2009/V3/I2/187
groupsnFEV1/LFEV1/FVC(%)Tim-3/β-actin (mRNA)
acute attack group232.29±0.81*?61.8±7.8*?0.39±0.06*?
remission group213.21±0.79*75.6±5.7*0.18±0.05*
control group173.65±0.7582.5±4.10.07±0.03
Tab.1  The pulmonary ventilation function and the Tim-3 mRNA expression in peripheral blood T lymphocytes
Fig.1  Tim-3 RT-PCR in peripheral blood T lymphocytes. 1: asthma acute attack group; 2: asthma remission group; 3: normal control group; M: marker.
groupsnIL-4IFN-γ
acute attack group2368.42±10.54*?65.74±7.85*?
remission group2141.83±9.37*120.84±11.62*
control group1732.75±8.16139.65±13.47
Tab.2  IL-4 and IFN-γ concentrations in the peripheral blood T lymphocytes pg/mL
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