%A Xiaohua PAN, Shuhua YANG, Deming XIAO, Yong DAI, Lili REN %T Gene expression disparity in giant cell tumor of bone %0 Journal Article %D 2009 %J Front. Med. %J Frontiers of Medicine %@ 2095-0217 %R 10.1007/s11684-009-0012-y %P 49-56 %V 3 %N 1 %U {https://academic.hep.com.cn/fmd/EN/10.1007/s11684-009-0012-y %8 2009-03-05 %X
The aim of this paper was to study the differential gene expression of giant cell tumor of bone (GCTB) by gene chip technology. Total RNA of 8 fresh GCTB specimens (Jaffe I∶6 cases, II∶1 case, III∶1 case; Campanacci I∶6 cases, II∶1 case, III∶1 case; Enneking Staging G0T1-2M0: 5 cases, G1T1-2M0: 2 cases, G1T2M0: 1 case) and 4 normal bony callus specimens (the control group) were extracted and purified to get mRNA and then reverse transcribed to complementary DNA, respectively. Microarray screening with a set of 8064 human cDNA genes was conducted to analyze the difference among the samples and the control. The hybridization signals were scanned. The gene expression disparity between the GCTB samples and normal bony callus was significantly different (