%A LEI Caixia, ZHANG Wei, SUN Xiaowei, DU Guoping, WANG Li, LIU Yinkun %T Effects of galectin-3 inhibition on endometrial cell cycle and adhesion %0 Journal Article %D 2007 %J Front. Med. %J Frontiers of Medicine %@ 2095-0217 %R 10.1007/s11684-007-0076-5 %P 390-397 %V 1 %N 4 %U {https://academic.hep.com.cn/fmd/EN/10.1007/s11684-007-0076-5 %8 2007-12-05 %X Galectin-3 (gal-3) and its ligands have been implicated in cell transformation and cancer metastasis. Gal-3 protein has been found in uterine epithelial cells adjacent to implanting blastocysts in different cell types. In order to investigate the role of gal-3 in the establishment of human endometrial receptivity, the expression of gal-3 in human endometrial cell line RL95-2 was silenced by RNA interference technology using gal-3 specific small RNA. The expression of gal-3 was detected by the reverse transcriptase-polymerase chain reaction and Western blot analysis. After the suppression of gal-3, cell cycle changes and the expression of integrin β1 were detected by flow cytometry. The adhesive ability of RL95-2 cells was analyzed by the adhesion test. Gal-3 siRNA transfection efficiency reached 70%–90%. The expression of gal-3 mRNA and protein in RL95-2 cells was strongly inhibited by 70%–90% after RNA interference. Inhibition of gal-3 expression decreased S-phase but increased G1 phase cells. Integrin β1 expression was down-regulated, and the adhesive ability of RL95-2 cells to fibronectin (FN) was significantly reduced. Gal-3 may be involved in the establishment of endometrial receptivity by regulating the proliferation and adhesion of endometrial cells. The influence on adhesion may be related with the integrin modulation.