1 Institute of Stomatology, Nanjing Medical University, Nanjing, 210029, China 2 Endodontic Department, Affiliated School of Stomatology, Nanjing Medical University, Nanjing, 210029, China
Effects of mineral trioxide aggregate on the expression of TNF α and IL 1 in human stem cells from apical papilla
摘要:Objective To determine the effects of mineral trioxide aggregate （MTA） on the expression of TNF α and IL 1 in human stem cells from apical papilla （SCAPs）.Methods SCAPs were isolated by magnetic activated cell sorting （MACS） and cultured in α MEM medium containing 2mg/mL MTA for 1 day and 3 days.Enzyme linked immunosorbent assay （ELISA） was applied to examine the TNF α and IL 1 in the culture supernatant of SCAPs.Quantitative RT PCR was used to determine the gene expression of TNF α, IL 1α, and IL 1β in SCAPs.Results The expression levels of TNF α and IL 1 at both protein expression and mRNA transcription were inhibited （P<001） when the cells were treated with MTA.Conclusion MTA can inhibit the expression of early stage inflammatory cytokines and relieve the inflammation via reducing the secretion of inflammatory cytokines.
出版日期: 2015-04-22
引用本文:
Yanping Wang,Yangyu Zheng,Shuanglin Jin,Ming Yan,Hu Li,Jinhua Yu. Effects of mineral trioxide aggregate on the expression of TNF α and IL 1 in human stem cells from apical papilla[J]. 转化医学研究, 2013, 3(1): 18-22.
