1. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China; 2. Department of Food Science, Hebei North University, Zhangjiakou 075000, China; 3. College of Traditional Chinese Veterinary Science, Agricultural University of Hebei, Dingzhou 073000, China
The objective of this research was to develop a detection method for Eperythrozoon wenyoni infection using polymerase chain reaction (PCR) assay technique. A pair of primers was designed and synthesized according to the conservative sequence 16S rRNA. The PCR assay was performed with the primers. A 985-bp fragment was amplified by using PCR. The amplified fragments with the expected size were identified by EcoR I restriction digestion. The crossing-reaction, specific-reaction and duplicate-reaction indicated that the PCR method is a specific, sensitive, fast and effective method for diagnosing E. wenyoni infection at group level.
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