Abstract：By screening samples from different sources, a high-yield yet low-cost lipase of Enterobacter aerogenes was found and used in biodiesel production. The enzyme activity of Enterobacter aerogenes reached 34.16 U·mL−1 under the optimum conditions of initial pH 9.0, agitating at 30°C for 40 h, using NaHCO3 and glucose as carbon sources and peptone as nitrogen source, with 0.1% (w/v) MgSO4·7H2O. When adding 1.0% (w/v) olive oil to the culture, the enzyme activity reached 66.31 U·mL−1, which increased by 56.5% compared to that obtained by using 2.0% (w/v) olive oil as the sole carbon source. The lipase of Enterobacter aerogenes was immobilized by diatomite and used to catalyze biodiesel with rapeseed oil as raw materials, the transesterification rate was 92.91% under the optimum reaction conditions, which were using 1000 U immobilized lipase and 5 mL n-hexane, with ethanol as acyl acceptors, at the molar ratio of oil/ethanol of 1∶4, and adding ethanol twice, at 35°C for 48h. Therefore, the Enterobacter aerogenes will potentially serve as a promising alternative lipase for biodiesel production.
Andrea S, Marcella P, Maura M, Vincenzo S (2008). Comparison among immobilised lipases on macroporous polypropylene toward biodiesel synthesis. Journal of Molecular Catalysis B: Enzymatic, 54: 19–26 doi: 10.1016/j.molcatb.2007.12.006
Burkert J F M, Maugeri F, Rodrigues M I (2004). Optimization of extracellular lipase production by Geotrichum sp. using factorial design. Bioresour Technol, 91: 77–84 doi: 10.1016/S0960-8524(03)00152-4
Camargo de Morais M M, Morais Jr M A, Melo E H M, Ledingham W M, Lima Filho J L (1998). Production of extracellular lipase by a Candida rugosa strain isolated in Pernambuco. Bras Rev Microb, 29: 134–137
Castro-Ochoa L D, Rodríguez-Gómez C, Valerio-Alfaro G, Oliart Ros R (2005). Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11. Enzyme Microb Technol, 37: 648–654 doi: 10.1016/j.enzmictec.2005.06.003
Chen Y M, Xiao B, Jie C, Fu Y, Lv P, Wang X W (2009). Synthesis of biodiesel from waste cooking oil using immobilized lipase in fixed bed reactor. Energy Conversion and Management, 50: 668–673 doi: 10.1016/j.enconman.2008.10.011
Freedman B, Pryde E H, Mounts T L (1984). Mounts variables affecting the yields of fatty esters from transesterified vegetableoils. J Am Oil Chem Soc, 61: 1638–43 doi: 10.1007/BF02541649
Funda Y, Dilek K, Akin A N (2007). Biodiesel production from waste oils by using lipase immobilized on hydrotalcite and zeolites. J Chem Engineer, 134 262–267 doi: 10.1016/j.cej.2007.03.041
Gulati R, Saxena R K, Gupta R, Yadav R P, Davidson W S (2000). Parametric optimization of Aspergillus terreus lipase production and its potential in ester synthesis. Process Biochem, 35: 459–464 doi: 10.1016/S0032-9592(99)00090-4
Haba E, Bresco O, Ferrer C, Marqué A, Busquets M, Manresa A (2000). Isolation of lipase-secreting bacteria by deploying used frying oil as selectivesubstrate. Enzyme Microb Technol, 26: 40–44 doi: 10.1016/S0141-0229(99)00125-8
Houria A, Abel H, Valerie D, Comeau L (2002). Isolation and characterization of an extracellular lipasefrom Mucor sp. strain isolatedfrom palm fruit. Enzyme Microb Technol, 31: 968–975 doi: 10.1016/S0141-0229(02)00190-4
Lin E S, Wang C C, Sung S C (2006). Cultivating conditions influence lipase production by the edible Basidiomycete Antrodia cinnamomea in submerged culture. Enzyme Microb Technol, 39: 98–102 doi: 10.1016/j.enzmictec.2005.10.002
Maia M M D, Heasley A, Camargo de Morais M M, Melo E H M, Morais Jr M A, Ledingham W M, Lima Filho J L (2001). Effect of culture conditions on lipase production by Fusarium solani in batch fermentation. Bioresour Technol, 76: 23–27 doi: 10.1016/S0960-8524(00)00079-1
Marija A, Ivana L, Tamara K, Ljubinka V, Wolfram S, Jasenka P (1999). Purification and properties of extracellularlipase from Streptomyces rimosus, Enzyme Microb Technol, 25: 522–529 doi: 10.1016/S0141-0229(99)00077-0
Mitsuhiro O, Masaru K, Shinji H, Hideki Y, Akihiko K, Eiji I, Hideki F (2005). Facilitatory effect of immobilizedlipase-producing Rhizopus oryzae cells on acyl migration in biodiesel-fuel production. J Biochem Engineer, 23: 45–51 doi: 10.1016/j.bej.2004.10.009
Nadir D, Bülent K (2008). Enzymatic production of biodiesel from canola oil usingimmobilized lipase. Biomass and Bioenerg, 32: 1274–1278 doi: 10.1016/j.biombioe.2008.03.005
Noureddini H, Gao X, Philkana R S (2005). Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil. Bioresour Technol, 96: 769–777 doi: 10.1016/j.biortech.2004.05.029
Pimentel M C B, Melo E H M, Filho J L, Durán N (1996). Production of lipase free of citrinin by Penicilium citrinum. Mycopathologia, 133: 119–121 doi: 10.1007/BF00439123
Rodriguez J A, Mateos J C, Nungaray J, González V, Bhagnagar T, Roussos S, Cordova J, Baratti J (2006). Improving lipase production by nutrient source modificationusing Rhizopus homothallicus cultured in solid state fermentation. Process Biochem, 41: 2264–2269 doi: 10.1016/j.procbio.2006.05.017
Rohit S, Yusuf C, Uttam C B (2001). Production, purification, characterization, and applications of lipases. Biotechnol Adv, 19: 627–662 doi: 10.1016/S0734-9750(01)00086-6
Sevgi E, GönÜl D, Serpil T (2007). Isolation of lipase producing Bacillus sp. from olive mill wastewater and improving its enzyme activity. J Hazard Mater, 149: 720–724 doi: 10.1016/j.jhazmat.2007.04.034
Shweta P, Shamsher S K, Ghanshyam C, Gupta R (2008). Glutaraldehyde activation of polymer Nylon-6 for lipase immobilization: Enzyme characteristics and stability. Bioresour Technol, 99: 2566–2570 doi: 10.1016/j.biortech.2007.04.042
Taichi S, Masaru K, Takeshi M, Kazuhiro B, Akihiko K, Yuji S, Hideo N, Hideki F (2000). Pretreatment of immobilized Candida antarctica lipase for biodiesel fuel productionfrom plant oil. J Biosci Bioeng, 90: 180–183
Tan T W, Zhang M, Wang B W, Li D (2003). Screening of high lipase producing Candida sp. and productionof lipase by fermentation. Process Biochemistry, 39: 459–465 doi: 10.1016/S0032-9592(03)00091-8
Watanabe Y, Shimada Y, Sugihara A, Tominaga Y (2002). Conversion of degummed soybean oil to biodiesel fuel with immobilized Candida antarctica lipase. J Mole Catal B: Enzymatic, 17: 151–155 doi: 10.1016/S1381-1177(02)00022-X
