1.Molecular Plant Pathology
Lab., College of Life Science, Agricultural University of Hebei, Baoding
071001, China; 2.National Millet Improvement
Center, Institute of Millet Crops, Hebei Academy of Agriculture and
Forestry Sciences, Shijiazhuang 050000, China;
Abstract：RUS1 was one of the disease resistance gene analogs obtained from Setaria italica Beauv. Semi-quantitative RT-PCR analysis result showed that RUS1 gene could be induced by Uromyces setariae-italicae and had relation to the resistance response of Setaria italica Beauv. against Uromyces setariae-italicae infection. Promoter sequence of RUS1 was obtained by the method of Genome Walking, and its length was 675 bp. RUS1 promoter and pCAMBIA1300 vector were fused to construct RUS1∷GUS vector. GUS histochemical staining result showed that promoter could activate gene expression. RUS1 gene (including the promoter sequence) was obtained through PCR amplification and then fused with pCAMBIA1300 vector to construct pCAMBIA1300∶RUS1 plant expressing vector. The research laid a foundation for gene functional identification of RUS1.
Catherine F, Silvia T, Nils S, Laurence A, Aure L N, Beat K(2003). Map-based isolation of the leaf rustdisease resistance gene Lr10 fromthe hexaploid wheat (Triticum aestivum L.) genome. Proceedings of the NationalAcademy of Sciences of the United States of America, 100: 15253–15258 doi: 10.1073/pnas.2435133100
Ching-I A W, Gregor G, Jade K F, Trazel T, AnnMaree C, Gregory J L, Fionna E L, Joel P M, Horst J S, Peter A A, Jeffrey G E, Peter N D, Boštjan K(2007). Crystalstructures of flax rust avirulence proteins avr L567-A and-D revealdetails of the structural basis for flax disease resistance specificity. The Plant Cell, 19: 2898–2912 doi: 10.1105/tpc.107.053611
Corderode M M, Santiago D N, Pliego A F, Quesada M A, Mercado J A(2004). The CaMV35S promoter is highly active on floral organs and pollen of transgenicstrawberry plants. Plant Cell Report, 23(1―2): 32–38
Craig A W, Todd E R, Nicholas C C, Marie N, Harold N T, Tony P, Scot H H(2003). Geneticand molecular characterization of the maize rp3 rust resistance locus. Genetics, 162: 381–394
Filho F A, Schinor E H, Paoli L G, Mendes B M J, Harakava R, Dean W G, Richard F L(2006). GUS gene expression driven by a citrus promoter in transgenictobacco and ‘Valencia’ sweet orange. Pesqisa Agropecuária Brasilcira, 41(11): 1623–1628
Guan X, Stege J, Kim M, Dahmani Z, Fan N, Heifetz P, Barbas C F, Briggs S P(2002). Heritableendogenous gene regulation in plants with designed polydactyl zincfinger transcription factors. Proceedingsof the National Academy of Sciences of the United States of America, 99(20): 13296–13301 doi: 10.1073/pnas.192412899
He C Y, Zhang Z Y, Chen S Y(2001). Isolation and characterization ofsoybean NBS analogs. Chinese Science Bulletin, 46(23): 1984–1988 doi: 10.1007/BF02901912
Hull R, Covey S N, Dale P(2002). Genetically modified plants and the35S promoter: assessing the risk and enhancing the debate. Microbial Ecology in Health and Disease, 12: 1–5
Li H, Steven A B, Li W L, John P F, Harold N T, Bikram S G(2003). Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genomeof bread wheat. Genetics, 164: 655–664
Liu Y H, Yu J J, Ao G M, Zhao Q(2007). Factors influencing agrobacterium-mediated transformation of foxtailmillet (Setaria italica). Chinese Journal of Biochemistry and Molecular Biology, 23(7): 531–536 (in Chinese)
Nagadhara D, Ramesh S, Pasalu I C, Kondala R Y, Krishnaiah N V, Sarma N P, Bown D P, Gatehouse J A, Reddy V D, Rao K V(2003). Transgenic indica rice resistantto sap-sucking insects. Plant BiotechnologyJournal, 1: 231–240 doi: 10.1046/j.1467-7652.2003.00022.x
Pileggi M, Pereira A A M, Silva J D S, Pileggi S A V, Varma D P S(2001). An improvedmethod for transformation of lettuce by Agrobacteriumtumefaciens with a gene that confers freezing resistance. Brazilian Archives of Biology and Technology, 44(2): 191–196 doi: 10.1590/S1516-89132001000200013
Solís J I F, Mlejnek P, Studená K, Procházka S(2003). Application of sonication-assistedAgrobacterium-mediated transformation in Chenopodiumrubrum L. Plant Soil Environment, 49(6): 255–260
Song P, Heinen J L, Burns T H, Allen R D(2000). Expression of two tissue-specific promoters in transgenic cottonplants. The Journal of Cotton Science, 4: 217–223
Stangeland B, Salehian Z(2002). An improvedclearing method for GUS assay in Arabidopsis endosperm and seeds. Plant MolecularBiology Reporter, 20: 107–114 doi: 10.1007/BF02799426
Wang B J, Zhang Z G, Li X G, Wang Y J, He C Y, Zhang J S, Chen S Y(2003). Cloningand analysis of a disease resistance gene homolog from soybean. Acta Botanica Sinica, 45(7): 864–870
Wu J H, Luo X L, Guo H N, Xiao J L, Tian Y C(2006). Transgenic cotton,expressing Amaranthus caudatus agglutinin,confers enhanced resistance to aphids. Plant Breeding, 125: 390–394 doi: 10.1111/j.1439-0523.2006.01247.x
Yao J H, Pang Y Z, Qi H X, Wan B L, Zhao X Y, Kong W W, Sun X F, Tang K X(2003). Transgenic tobacco expressing Pinellia ternata agglutinin confers enhanceresistance to aphids. Transgenic Research, 12: 715–722 doi: 10.1023/B:TRAG.0000005146.05655.7d
