Construction of RNAi vector for flower-related gene and verifications of the mutant in <italic>Arabidopsis thaliana

doi:10.1007/s11703-011-1094-2

Frontiers of Agriculture in China

2011, Vol. 5

Issue (3): 333-337 https://doi.org/10.1007/s11703-011-1094-2

RESEARCH ARTICLE

本期目录

Construction of RNAi vector for flower-related gene and verifications of the mutant in Arabidopsis thaliana

Jing ZHANG, Jihong XING(

), Jingao DONG(

)

Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding 071001, China

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Abstract：

The timing of floral transition is tightly controlled by a combination of endogenous and environmental signals. One early flowering mutant plant was screened from Arabidopsis library of T-DNA insertion to accelerate flowering under short-day condition, and a related-gene EFS1 (AT4G36680.1) was isolated and identified as a novel flowering-time gene of Arabidopsis in our preliminary studies. To investigate the function and the specific mechanism of EFS1 in the flower process control, the RNAi expression vector containing EFS1 gene-specific sequences in the sense and antisense orientations was constructed and transferred into Arabidopsis by using the floral-dip method, with 11 transgenic plants obtained through hygromycin B screening and PCR assays. The results showed that the expression level of EFS1 in transgenic lines was significantly lower than that in wild type and efs1 mutant. The flowering time of the efs1 mutant and RNAi transgenic plants was much earlier than that of wild-type plants. This result further verified that the EFS1 gene played an important role in flowering, and its specific mechanisms need further study. These work provided a foundation to further regulatory mechanisms of EFS1 in the control of floral transition.

Key words： Arabidopsis thaliana RNAi EFS1 gene early flowering

收稿日期: 2011-01-10 出版日期: 2011-09-05

Corresponding Author(s): XING Jihong,Email:xingjihong2000@126.com; DONG Jingao,Email:shmdjg@hebau.edu.cn

引用本文:

. Construction of RNAi vector for flower-related gene and verifications of the mutant in Arabidopsis thaliana[J]. Frontiers of Agriculture in China, 2011, 5(3): 333-337.
Jing ZHANG, Jihong XING, Jingao DONG. Construction of RNAi vector for flower-related gene and verifications of the mutant in Arabidopsis thaliana. Front Agric Chin, 2011, 5(3): 333-337.

链接本文:

https://academic.hep.com.cn/fag/CN/10.1007/s11703-011-1094-2
https://academic.hep.com.cn/fag/CN/Y2011/V5/I3/333

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