To investigate the frequency of occurrence and the concentrations of aflatoxins (AFs), deoxynivalenol (DON), zearalenone (ZEN) and fumonisins (FBs) in naturally infected maize, 25 samples of maize collected from fields in Hebei Province, China, were analyzed by high-performance liquid chromatography (HPLC). The maize samples were found to be frequently contaminated with DON (68%), ZEN (60%) and FBs (32%) in the range of from 28 to 2533 μg/kg, 60 to 1239 μg/kg and 150 to 4480 μg/kg, respectively. The average concentration found for DON, ZEN and FB1+ FB2 were 605, 238 and 418 μg/kg, respectively. The average concentration of DON (605 μg/kg) in our samples was below the maximum tolerable limit of 1000 μg/kg set as the Chinese standard for maize, while ZEN (238 μg/kg) was almost four times as high as the maximum tolerable limit of 60 μg/kg. The overall level of FB (FB1+ FB2) contamination was relatively low, with an average concentration of 418 μg/kg in 32% (8 of 25) of maize samples from Hebei. AFs were not detected in any of the tested samples. This is the first report on the natural occurrence of multimycotoxin in maize in China.
Corresponding Author(s):
LIU Yingchao,Email:liuyingchao@hebau.edu.cn; DONG Jingao,Email:dongjingao@126.com
引用本文:
. Occurrence of major mycotoxins in maize from Hebei Province, China[J]. Frontiers of Agriculture in China, 2011, 5(4): 497-503.
Yizhi FENG, Bu TAO, Minhao PANG, Yingchao LIU, Jingao DONG. Occurrence of major mycotoxins in maize from Hebei Province, China. Front Agric Chin, 2011, 5(4): 497-503.
Abou-Arab A A K, Soliman Kawther M, EI Tantawy M E, Badeaa R I, Khayria N (1999). Quantity estimation of some contaminants in commonly used medicinal plants in the Egyptian market. Food Chem , 67(4): 357-363 doi: 10.1016/S0308-8146(99)00082-5
2
Bankole S A (1995). Mycoflora, mycotoxin producing fungi and mycotoxins from melon ball snacks in Nigeria. Chemie, Mikrobiologie, Technologie der Lebensmittel , 17: 164-168
3
Bankole S A, Ogunsanwo B M, Mabekoje O O (2004). Natural occurrence of moulds and aflatoxin B1 in melon seeds from markets in Nigeria. Food Chem Toxicol , 42(8): 1309-1314 doi: 10.1016/j.fct.2004.03.015 pmid:15207382
Berthiller F, Dall’asta C, Corradini R, Marchelli R, Sulyok M, Krska R, Adam G, Schuhmacher R (2009). Occurrence of deoxynivalenol and its 3-beta-D-glucoside in wheat and maize. Food Addit Contam Part A Chem Anal Control Expo Risk Assess , 26(4): 507-511 pmid:19680925
6
Betina V (1989). Biological effects of mycotoxins. In: Betina V, ed. Mycotoxins: Chemical, Biological and Environmental Aspects . Amsterdam: Elsevier Science Publishers, 42-58
7
Castellá G, Bragulat M R, Caba?es F J (1999). Surveillance of fumonisins in maize-based feeds and cereals from Spain. J Agric Food Chem , 47(11): 4707-4710 doi: 10.1021/jf981236d pmid:10552876
8
Chu F S, Li G Y (1994). Simultaneous occurrence of fumonisin B1 and other mycotoxins in moldy corn collected from the People’s Republic of China in regions with high incidences of esophageal cancer. Appl Environ Microbiol , 60(3): 847-852 pmid:8161178
9
Doko M B, Visconti A (1994). Occurrence of fumonisins B1 and B2 in corn and corn-based human foodstuffs in Italy. Food Addit Contam , 11(4): 433-439 pmid:7958113
10
Domijan A M, Peraica M, Jurjevi? ?, Ivi? D, Cvjetkovi? B (2005). Fumonisin B1, fumonisin B2, zearalenone and ochratoxin A contamination of maize in Croatia. Food Addit Contam , 22(7): 677-680 doi: 10.1080/02652030500132927 pmid:16019843
11
Elshafie A E, Al-Rashdi T A, Al-Bahry S N, Bakheit C S (2002). Fungi and aflatoxins associated with spices in the Sultanate of Oman. Mycopathologia , 155(3): 155-160 doi: 10.1023/A:1020427527963 pmid:12617502
12
European Commission (EC) (2007). Commission Regulation (EC) No 1126/2007, of 28 September 2007 amending Regulation (EC) No1881/2006 setting maximum levels for certain contaminants in foodstuffs as regards Fusarium toxins in maize and maize products. Off J Eur Union , L255: 14-17
13
Fandohan P, Gnonlonfin B, Hell K, Marasas W F O, Wingfield M J (2005). Natural occurrence of Fusarium and subsequent fumonisin contamination in preharvest and stored maize in Benin, West Africa. Int J Food Microbiol , 99(2): 173-183 doi: 10.1016/j.ijfoodmicro.2004.08.012 pmid:15734565
14
GB (2005). Hygienic standardard for gains (GB2715-2005). In: Mycotoxin limits, Beijing, Standardization Administration of the People’s Republic of China , 2 (in Chinese)
15
Gelderblom W C, Jaskiewicz K, Marasas W F, Thiel P G, Horak R M, Vleggaar R, Kriek N P (1988). Fumonisins—novel mycotoxins with cancer-promoting activity produced by Fusarium moniliforme. Appl Environ Microbiol , 54(7): 1806-1811 pmid:2901247
16
Gong H Z, Ji R, Li Y X, Zhang H Y, Li B, Zhao Y, Sun L, Yu F, Yang J (2009). Occurrence of fumonisin B(1) in corn from the main corn-producing areas of China. Mycopathologia , 167(1): 31-36 doi: 10.1007/s11046-008-9146-8 pmid:18636342
17
González H H L, Martínez E J, Pacin A M, Resnik S L, Sydenham E W (1999). Natural co-occurrence of fumonisins, deoxynivalenol, zearalenone and aflatoxins in field trial corn in Argentina. Food Addit Contam , 16(12): 565-569 doi: 10.1080/026520399283704 pmid:10789378
18
Groopman J D, Donahue K F (1988). Aflatoxin, a human carcinogen: determination in foods and biological samples by monoclonal antibody affinity chromatography. J Assoc Off Anal Chem , 71(5): 861-867 pmid:3235405
19
Haschek W M, Gumprecht L A, Smith G, Tumbleson M E, Constable P D (2001). Fumonisin toxicosis in swine: an overview of porcine pulmonary edema and current perspectives. Environ Health Perspect , 109(Suppl 2): 251-257 doi: 10.2307/3435016 pmid:11359693
20
Horn B W, Wicklow D T (1983). Factors influencing the inhibition of aflatoxin production in corn by Aspergillus niger. Can J Microbiol , 29(9): 1087-1091 doi: 10.1139/m83-167 pmid:6418365
21
IARC (2002). Monograph on the Evaluation of Carcinogenic Risk to Humans. In: Some Traditional Herbal Medicines, Some Mycotoxins, Naphtalene and Styrene , vol. 82. Lyon: IARC, 171-300
22
Jaji? I, Juri? V, Glamoci? D, Abramovi? B (2008). Occurrence of deoxynivalenol in maize and wheat in Serbia. Int J Mol Sci , 9(11): 2114-2126 doi: 10.3390/ijms9112114 pmid:19330062
23
Kimanya M E, De Meulenaer B, Tiisekwa B, Ugullum C, Devlieghere F, Van Camp J, Samapundo S, Kolsteren P (2009). Fumonisins exposure from freshly harvested and stored maize and its relationship with traditional agronomic practices in Rombo district, Tanzania. Food Addit Contam , 26(8): 1199-1208 doi: 10.1080/02652030902922784
24
Kurtzman C P, Horn B W, Hesseltine C W (1987). Aspergillus nomius, a new aflatoxin-producing species related to Aspergillus flavus and Aspergillus tamarii. Antonie van Leeuwenhoek , 53(3): 147-158 doi: 10.1007/BF00393843 pmid:3116923
25
Lino C M, Silva L J G, Pena A L S, Silveira M I (2006). Determination of fumonisins B1 and B2 in Portuguese maize and maize-based samples by HPLC with fluorescence detection. Anal Bioanal Chem , 384(5): 1214-1220 doi: 10.1007/s00216-005-0295-z pmid:16465502
26
Lino C M, Silva L J G, Pena A, Fernández M, Ma?es J (2007). Occurrence of fumonisins B1 and B2 in broa, typical Portuguese maize bread. Int J Food Microbiol , 118(1): 79-82 doi: 10.1016/j.ijfoodmicro.2007.04.014 pmid:17604863
27
Marin D E, Gouze M E, Taranu I, Oswald I P (2007). Fumonisin B1 alters cell cycle progression and interleukin-2 synthesis in swine peripheral blood mononuclear cells. Mol Nutr Food Res , 51(11): 1406-1412 doi: 10.1002/mnfr.200700131 pmid:17918165
28
Marinamartins H, Almeida I, Marques M F, Guerra M M (2008). Fumonisins and deoxynivalenol in corn-based food products in Portugal. Food Chem Toxicol , 46(7): 2585-2587 doi: 10.1016/j.fct.2008.03.030 pmid:18486293
29
Martins H M, Guerra M M M, Bernardo F M D (2007). Presencia de aflatoxina B1 en piensos para ganado lechero en Portugal durante el periodo 1995-2004. Rev Iberoam Micol , 24(1): 69-71 17592898 doi: 10.1016/S1130-1406(07)70017-7
30
Missmer S A, Suarez L, Felkner M, Wang E, Merrill A H Jr, Rothman K J, Hendricks K A (2006). Exposure to fumonisins and the occurrence of neural tube defects along the Texas-Mexico border. Environ Health Perspect , 114(2): 237-241 doi: 10.1289/ehp.8221 pmid:16451860
31
Monbaliu S, Van Poucke C, Detavernier C, Dumoulin F, Van De Velde M, Schoeters E, Van Dyck S, Averkieva O, Van Peteghem C, De Saeger S (2010). Occurrence of mycotoxins in feed as analyzed by a multi-mycotoxin LC-MS/MS method. J Agric Food Chem , 58(1): 66-71 doi: 10.1021/jf903859z pmid:19994896
32
Ok H E, Kim H J, Shim W B, Lee H, Bae D H, Chung D H, Chun H S (2007). Natural occurrence of aflatoxin B1 in marketed foods and risk estimates of dietary exposure in Koreans. J Food Prot , 70(12): 2824-2828 pmid:18095437
33
Pestka J J (2007). Deoxynivalenol: Toxicity, mechanisms and animal health risks. Anim Feed Sci Technol , 137(3-4): 283-298 doi: 10.1016/j.anifeedsci.2007.06.006
34
Salem N M, Ahmad R (2010). Mycotoxins in food from Jordan: preliminary survey. Food Contr , 21(8): 1099-1103 doi: 10.1016/j.foodcont.2010.01.002
35
Schaafsma A W, Nicol R W, Savard M E, Sinha R C, Reid L M, Rottinghaus G (1998). Analysis of Fusarium toxins in maize and wheat using thin layer chromatography. Mycopathologia , 142(2): 107-113 doi: 10.1023/A:1006937720711 pmid:9926423
36
Scudamore K A, Patel S (2000). Survey for aflatoxins, ochratoxin A, zearalenone and fumonisins in maize imported into the United Kingdom. Food Addit Contam , 17(5): 407-416 doi: 10.1080/026520300404824 pmid:10945108
37
Scudamore K A, Patel S (2009). Occurrence of Fusarium mycotoxins in maize imported into the UK, 2004-2007. Food Addit Contam Part A Chem Anal Control Expo Risk Assess , 26(3): 363-371 pmid:19680910
38
Shim W B, Kim K Y, Chung D H (2009). Development and validation of a gold nanoparticle immunochromatographic assay (ICG) for the detection of zearalenone. J Agric Food Chem , 57(10): 4035-4041 doi: 10.1021/jf900075h pmid:19348422
39
Silva C M G, Vargas E A (2001). A survey of zearalenone in corn using Romer MycosepTM 224 column and high performance liquid chromatography. Food Addit Contam , 18(1): 39-45 doi: 10.1080/02652030010002649 pmid:11212546
40
Silva L J G, Lino C M, Pena A, Moltó J C (2007). Occurrence of fumonisins B1 and B2 in Portuguese maize and maize-based foods intended for human consumption. Food Addit Contam , 24(4): 381-390 doi: 10.1080/02652030601003811 pmid:17454111
41
Simon P, Delsaut P, Lafontaine M, Morele Y, Nicot T (1998). Automated column-switching high-performance liquid chromatography for the determination of aflatoxin M1. J Chromatogr B Biomed Sci Appl , 712(1-2): 95-104 doi: 10.1016/S0378-4347(98)00139-X pmid:9698232
42
Thongrussamee T, Kuzmina N S, Shim W B, Jiratpong T, Eremin S A, Intrasook J, Chung D H (2008). Monoclonal-based enzyme-linked immunosorbent assay for the detection of zearalenone in cereals. Food Addit Contam , 25(8): 997-1006 doi: 10.1080/02652030701823159
43
Trung T S, Tabuc C, Bailly S, Querin A, Guerre P, Bailly J D (2008). Fungal mycoflora and contamination of maize from Vietnam with aflatoxin B1 and fumonisin B1. World Mycotoxin Journal , 1(1): 87-94 doi: 10.3920/WMJ2008.x010
44
Visconti A, Pascale M (1998). Determination of zearalenone in corn by means of immunoaffinity clean-up and high-performance liquid chromatography with fluorescence detection. J Chromatogr A , 815(1): 133-140 doi: 10.1016/S0021-9673(98)00296-9 pmid:9718713
45
Wang Y, Chai T, Lu G, Quan C, Duan H, Yao M, Zucker B A, Schlenker G (2008). Simultaneous detection of airborne aflatoxin, ochratoxin and zearalenone in a poultry house by immunoaffinity clean-up and high-performance liquid chromatography. Environ Res , 107(2): 139-144 doi: 10.1016/j.envres.2008.01.008 pmid:18313042
46
Yang L, Wang L, Pan J Y, Xiang L, Yang M H, Logrieco A F (2010). Determination of ochratoxin A in traditional Chinese medicinal plants by HPLC-FLD. Food Addit Contam Part A Chem Anal Control Expo Risk Assess , 27(7): 989-997 pmid:20432098
47
Zinedine A, Brera C, Elakhdari S, Catano C, Debegnach F, Angelini S, Desantis B, Faid M, Benlemlih M, Minardi V, Miraglia M (2006). Natural occurrence of mycotoxins in cereals and spices commercialized in Morocco. Food Contr , 17(11): 868-874 doi: 10.1016/j.foodcont.2005.06.001
48
Zinedine A, Ma?es J (2009). Occurrence and legislation of mycotoxins in food and feed from Morocco. Food Contr , 20(4): 334-344 doi: 10.1016/j.foodcont.2008.07.002
