Study on separation and purification of oligomeric proanthocyanidin from Rhodiola rosea
Study on separation and purification of oligomeric proanthocyanidin from Rhodiola rosea
Zhiping YIN1, Boyang ZHANG2, Hongyu CHEN1, Sisi WANG1, Wen ZHAO1()
1. College of Food Science and Technology, Hebei Agriculture University, Engineering Technology Research Center for Agricultural Product Processing of Hebei, Baoding 071001, China; 2. College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
The objective was to study the technology for the separation and purification of oligomeric proanthocyanidins from Rhodiola rosea and establish the best operating conditions. First, the oligomeric proanthocyanidins was extracted by ethyl acetate from rough-extracted R. rosea liquid using an optimized technique. Its purification was achieved by macroporous resins. Five kinds of macroporous adsorbent resins were compared for the adsorption and desorption performance of procyanidins, the concentration and pH value of both the extracted sample and the eluant were investigated. According to the results, the optimized conditions were as follows: four times of extraction at 25 min each time was effective, the best volume ratio was 1.5:1 (ethyl acetate: extracted solution); AB-8 resin was the best choice; the concentration of the extracted sample was 4.0 g/L, and the pH was 4.5; the ratio of the diameter to height of the chromatography column was 1:40 (cm); 50% ethanol was used as the eluant at pH 5; and finally, the purity of procyanidins reached 88. 3%.
. Study on separation and purification of oligomeric proanthocyanidin from Rhodiola rosea[J]. Frontiers of Agriculture in China, 2011, 5(4): 637-642.
Zhiping YIN, Boyang ZHANG, Hongyu CHEN, Sisi WANG, Wen ZHAO. Study on separation and purification of oligomeric proanthocyanidin from Rhodiola rosea. Front Agric Chin, 2011, 5(4): 637-642.
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