Seed priming technique has been known to improve germination and seedling emergence under different environment stresses. The objective of this study was to evaluate the effectiveness of seed priming in improving the seed germination and seedling vigor of Agropyron elongatum and Bromus inermis in response to five concentrations of the allelopathic extract of Thymus kotschyanus (0, 5%, 25%, 50% and 75%) under laboratory conditions. Pretreatments included gibberellic acid (250, 500 and 1000 ppm), potassium nitrate (0.1%, 0.2% and 0.3%) and salicylic acid (100, 200 and 300 mg/L). This experiment was carried out as a factorial experiment based on a completely randomized design with four replications. The results showed that seed germination, germination speed and seedling vigor were significantly decreased (P<0.01) by increasing extract concentrations of Thymus kotschyanus allelopathic compounds in unprimed seed (control) compared with primed seeds. Seed priming enhanced germination percentage, germination speed and initial growth. Overall, the results of this study showed that the response of both species to pretreatment of seeds was positive. Therefore, pretreatments of seeds with the aforementioned chemical stimulators before planting can significantly reduce the negative effect of allelopathic compounds in Thymus kotschyanus during the germination stage and contribute to plant establishment by increasing germination speed and growth of embryos.
. Influence of some chemical compounds on germination and early seedling growth of two range species under allelopathic conditions[J]. Frontiers of Agriculture in China, 2011, 5(3): 310-321.
Morteza SABERI, Alireza SHAHRIARI, Farajollah TARNIAN, Mohammad JAFARI, Hanie SAFARI. Influence of some chemical compounds on germination and early seedling growth of two range species under allelopathic conditions. Front Agric Chin, 2011, 5(3): 310-321.
Albeles F B, Lonski J (1969). Stimulation of lettuce seed germination by ethylene. Plant Physiol , 44: 277–280 doi: 10.1104/pp.44.2.277
2
Balibrea M E, Dell’Amico J, Bolarín M C, Pérez-Alfocea F (2000). Carbon partitioning and sucrose metabolism in tomato plants growing under salinity. Physiollogia Plantarum, 110(4): 503–511 doi: 10.1111/j.1399-3054.2000.1100412.x
3
Barsa S M A, Pannu I A, Afzal I (2003). Evaluation of seedling vigor of hydro and matriprimed wheat (Triticum aestivum L.) seeds. International Journal of Agriculture and Biology , 5: 121–123
4
Borsani O, Valpuesta V, Botella M A (2001). Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings. Plant Physiol , 126(3): 1024–1030 doi: 10.1104/pp.126.3.1024 pmid:11457953
5
?irak C, Kevseroglu K, Ayan A K (2004). The effects of light and some presoaking treatments on germination rate of st. John Worth (Hypericum perforatum L.) seeds. Pakistan Journal of Biological Sciences, 7: 182–186 doi: 10.3923/pjbs.2004.182.186
6
Dat J F, Lopez-Delgado H, Foyer C H, Scott I M (1998). Parallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings. Plant Physiol , 116(4): 1351–1357 doi: 10.1104/pp.116.4.1351 pmid:9536052
7
Dra?i? G, Mihailovi? N, Loji? M (2006). Cadmium accumulation in Medicago sativa seedlings treated with salicylic acid. Biological plantrum, 50: 239–244 .
8
Dunand R T (1992). Enhancement of seedling vigor in rice (Oryza sativa L.) by seed treatment with gibberellic acid. In Karssen C M, van Loon L C, Vreugdenhil D, eds. Progress in Plant Growth Regulation.London:Kluwer Academic Publishers, 835–841
9
El-Khatib A A, Hegazy A K, Gala H K (2004). Does allelopathy have a role in the ecology of Chenopodium murale? Ann Bot Fenn , 41: 37–45
10
Escudero A, Albert M J, Pita J M, Pérez-García F (2000). Inhibitory effects of Artemisia herba alba on the germination of the gypsophyte Helianthemum squamatum. Plant Ecol, 148(1): 71–80 doi: 10.1023/A:1009848215019
11
Ghoulam C,Foursy A, Fares K (2002). Effects of salt stress on growth, inorganic ions and proline accumulation in relation to osmotic adjustment in five sugar beet cultivars. Environ Exp Bot , 47: 39–50
12
Glass A D M (1974). Influence of phenolic acids upon ion uptake. III. Inhibition of potassium absorption. J Exp Bot, 25: 1104–1113 doi: 10.1093/jxb/25.6.1104
13
Greipsson S (2001). Effects of stratification and GA3 on seed germination of a sand stabilising grass Leymus arenarius used in reclamation. Seed Sci & Technol , 29: 1–10
14
Horii A, McCue P, Shetty K (2007). Enhancement of seed vigour following insecticide and phenolic elicitor treatment. Bioresour Technol , 98(3): 623–632 doi: 10.1016/j.biortech.2006.02.028 pmid:16581243
15
Inderjit W J (2001). Soil: Environment effects on allelochemicals activity: Allelopathy in Natural and Managed Ecosystems. Agron J , 93: 79–84
16
Janda T, Szalai G, Antunovics Z, Horváth E, Páldi E (2000). Effect of benzoic acid and aspirin on chilling tolerance and photosynthesis in young maize plants. Maydica , 45: 29–33
17
Kang H M, Saltveit M E (2002). Chilling tolerance of maize, cucumber and rice seedling leaves and roots are differentially affected by salicylic acid. Physiol Plant , 115(4): 571–576 doi: 10.1034/j.1399-3054.2002.1150411.x pmid:12121463
18
Kaya M D, Ok?u G, Atak M, ??k?l? Y, Kolsar?c? ? (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Eur J Agron , 24(4): 291–295 doi: 10.1016/j.eja.2005.08.001
19
Kepczynski J, Groot S P C (1989). Key role for endogenous gibberellins in the germination of lettuce. Plant Physiol , 32: 314–319
20
Krant A,Yordanova R, Janda T, Szalai G, Popova L (2008). Treatment with salicylic acid decreases the effect of cadmium on photosynthesis in maize plants. J Plant Physiol , 165(9): 920–931 doi: 10.1016/j.jplph.2006.11.014 pmid:17913285
21
López M, Humara J M, Casares A, Majada J (2000). The effect of temperature and water stress on laboratory germination of Eucalyptus globulus Labill. seeds of different sizes. Annals of Forest Science, 57: 245–250
22
Pál M, Szalai G, Horváth E, Janda T, Páldi E (2002). Effect of salicylic acid during heavy metal stress. Acta Biologica Szegediensis , 46: 119–120
23
Popova L, Maslenkova L, Yordanova R, Krantev A, Szalai G, Janda T (2008). Salicylic acid protects photosynthesis against cadmium toxity in plants. Gen Appl Plant Physiol , 34: 133–148
24
Ritchie S, Gilroy S (1998). Gibberellins: regulating genes and germination. New Phytol , 140(3): 363–383 doi: 10.1046/j.1469-8137.1998.00299.x
25
Safari H, Tavili A, Saberi M (2010). Allelopathic effects of Thymus kotschyanus on seed germination and initial growth of Bromus tomentellus and Trifolium repens. Front Agric China , 4(4): 475–480 doi: 10.1007/s11703-010-1030-x
26
Senaratna T, Touchell D, Bunn E, Dixon K (2000). Acetyl salicylic acid (Aspirin) and salicylic acid induced multiple stress tolerance in bean and tomato plant. Plant Growth Regul , 30(2): 157–161 doi: 10.1023/A:1006386800974
27
Sharikova F M, Sakhabutdinova A R, Bezrukova M V, Fatkhutdinova R A, Fatkhudinova D R (2003). Changes in the hormonal status of wheat seedling induced by salicylic acid and salinity. Plant Sci , 164(3): 317–322 doi: 10.1016/S0168-9452(02)00415-6
28
Ta?gín E, Atící O, Nalbanto?lu B (2003). Effect of salicylic acid on freezing tolerance in winter wheat leaves. Plant Growth Regul , 41(3): 231–236 doi: 10.1023/B:GROW.0000007504.41476.c2
29
Zhang Y, Chen K, Zhang S, Ferguson I (2003). The role of salicylic acid in postharvest ripening of kiwifruit. Postharvest Biol Technol , 28(1): 67–74 doi: 10.1016/S0925-5214(02)00172-2
