Optimum nitrogen fertilization of <i>Calophyllum inophyllum</i> seedlings under greenhouse conditions

doi:10.15302/J-FASE-2016120

Front. Agr. Sci. Eng.

2016, Vol. 3

Issue (4) : 368-374 https://doi.org/10.15302/J-FASE-2016120

RESEARCH ARTICLE

Optimum nitrogen fertilization of Calophyllum inophyllum seedlings under greenhouse conditions

Wentao ZOU¹,Ruifeng JIA²,Jinchang YANG¹(

),Rongsheng LI¹,Guangtian YIN¹

¹. Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China
². Rural Work Bureau of High Tech Industry Development Zone in Huizhou, Huizhou 516005, China

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Abstract

A greenhouse pot experiment was conducted to study the effects of nitrogen fertilization on Calophy- llum inophyllum seedlings grown with 0, 50, 100, 150, 200, 300, 400 and 600 mg N per seedling according to exponential functions. Seedling height, root collar diameter, leaf area and total biomass increased with increasing fertilization from 0 to 200 mg N per seedling and decreased with further increase in fertilization from 300 to 600 mg N per seedling. The net photosynthetic rate, stomatal conductance, intercellular CO₂ concentration and transpiration rate of C. inophyllum seedlings showed a unimodal parabolic trend, with peak values of 7.29 mmol·m⁻²·s⁻¹, 0.071 mol·m⁻²·s⁻¹, 220 mmol·mol⁻¹ and 1.34 mmol·m⁻²·s⁻¹, respectively, when the rate of fertilization was 200 mg N per seedling. Photosynthetic gas exchange parameters were significantly different among nitrogen treatments. Based on the critical values of leaf N and P concentration and N/P ratio, the optimum amount of nitrogen of C. inophyllum seedlings was 200–400 mg per seedling for leaf N and P concentration, and 100–400 mg per seedling for N/P ratio. It was concluded that 200–400 mg N per seedling was the most suitable nitrogen range for C. inophyllum seedlings.

Keywords Calophyllum inophyllum growth nitrogen fertilization nutrient status photosynthesis

Corresponding Author(s): Jinchang YANG

Just Accepted Date: 09 December 2016 Online First Date: 04 January 2017 Issue Date: 22 January 2017

Cite this article:

Wentao ZOU,Ruifeng JIA,Jinchang YANG, et al. Optimum nitrogen fertilization of Calophyllum inophyllum seedlings under greenhouse conditions[J]. Front. Agr. Sci. Eng. , 2016, 3(4): 368-374.

URL:

https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2016120
https://academic.hep.com.cn/fase/EN/Y2016/V3/I4/368

Tab.1 Program for exponential nitrogen fertilization of Calophyllum inophyllum seedlings

Tab.2 Effects of nitrogen fertilizer treatments on growth of Calophyllum inophyllum seedlings

Fig.1 Effects of nitrogen fertilizer treatments on biomass of Calophyllum inophyllum seedlings. Different letters above the columns indicate significant differences between treatments according to Duncan’s Multiple Range Test (P<0.05). Data are means±SE (n = 4).

Fig.2 Effects of nitrogen fertilizer treatments on photosynthetic gas exchange parameters of Calophyllum inophyllum seedlings. (a) Net photosynthetic rate; (b) stomatal conductance; (c) intercellular CO₂ concentration; (d) transpiration rate. Different letters indicate significant differences between treatments according to Duncan’s Multiple Range Test (P<0.05). Data are means±SE (n = 4).

Tab.3 Effects of nitrogen fertilizer treatments on leaf nutrient situation of Calophyllum inophyllum seedlings

Fig.3 The quadratic relationship between leaf N and P concentrations, N/P ratio and biomass of Calophyllum inophyllum seedlings. (a) The relationship between leaf N and biomass; (b) the relationship between leaf P and biomass; (c) the relationship between leaf N/P ration and biomass.

Tab.4 Regression equations for nutrient concentration or ratio versus biomass of Calophyllum inophyllum seedlings

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