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Capillary electrophoresis as a tool for screening aptamer with high affinity and high specificity to ricin |
| TANG Jijun1, XIE Jianwei1, GUO Lei1, YAN Yan1, SHAO Ningsheng2 |
| 1.Beijing Institute of Pharmacology & Toxicology, Beijing 100850, China; 2.Beijing Institute of Basic Medical Sciences, Beijing 100850, China |
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Abstract Aptamers which specifically recognize targets are selected from random oligonucleotide library using systematic evolution of ligands by exponential enrichment (SELEX). In this paper, capillary electrophoresis (CE) as a separation approach has been introduced to SELEX procedure. The high efficiency of CE gives rise to greatly shorten the selection procedure. The results from enzyme-linked assay and dot blot experiment show that an enrichment pool has been obtained after four rounds selection, which can specifically recognize ricin.
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Issue Date: 05 December 2007
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