Kinetic analysis of interaction
between lipopolysaccharide and biomolecules

doi:10.1007/s11458-008-0005-4

Front. Chem. China

2008, Vol. 3

Issue (1) : 14-17 https://doi.org/10.1007/s11458-008-0005-4

Kinetic analysis of interaction between lipopolysaccharide and biomolecules

YANG Fan, YANG Xiurong

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences

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Abstract Lipopolysaccharide (LPS) is a major component of the outer membrane of all gram-negative bacteria. It interacts with some biomolecules and triggers a toxic reaction. In this paper, we studied the interaction between LPS from Salmonella Minnesota and some biomolecules using a surface plasmon resonance (SPR) biosensor. Biomolecules were immobilized on a CM5 sensor chip using the amino coupling method and LPS was injected over the immobilized surfaces. The affinity constant K_A of LPS with serum albumin, hemoglobin, chitosan and lysozyme was 2.36 × 10⁷, 2.03 × 10⁸, 7.58 × 10⁶, 2.82 × 10⁴ L·mol^-1, respectively. However, LPS could not interact with ferritin.

Issue Date: 05 March 2008

Cite this article:

YANG Xiurong,YANG Fan. Kinetic analysis of interaction between lipopolysaccharide and biomolecules[J]. Front. Chem. China, 2008, 3(1): 14-17.

URL:

https://academic.hep.com.cn/fcc/EN/10.1007/s11458-008-0005-4
https://academic.hep.com.cn/fcc/EN/Y2008/V3/I1/14

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