Study on eruption of heat for  B aroused by lanthanum nitrate
and its mechanism

doi:10.1007/s11458-008-0010-7

Front. Chem. China

2008, Vol. 3

Issue (1) : 70-75 https://doi.org/10.1007/s11458-008-0010-7

Study on eruption of heat for B aroused by lanthanum nitrate and its mechanism

LU Yan¹, LIU Guosheng², LIU Yi², RAN Zhilin³, WANG Hailei³, SHEN Ping⁴

1.College of Chemistry and Enviromental Sciences, Henan Normal University; 2.College of Chemistry and Molecular Sciences, Wuhan University; 3.College of Life Sciences, Henan Normal University; 4.College of Life Sciences, Wuhan University;

Download: PDF(177 KB) HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

Abstract Biological effect of rare-earth lanthanum nitrate on the growth of Escherichia coli B was studied using the calorimetric method. There were exceptional changes on the growth thermogenic curves for high concentrations of lanthanum nitrate. For example, the peak high, the total quantity of heat (Q) of cultures and the growth rate constants (k) are evidently increased when compared with normal E. coli B cultures. When the concentration of lanthanum nitrate was at 300 mg/L and 500 mg/L, the Q of the cultures reached 3.89 and 2.54 times of normal cultures, respectively. The survivability of cells and the biomass of the cultures were measured using biological methods and the results show that the growth and multiplication of cells were inhibited and that the biomass decreased at high concentration of lanthanum nitrate. These revealed that the inhibiting cells discharged more quantity of heat than the normal growing cells. We named this phenomenon as “eruption of heat”. It was suggested that the mechanism for the eruption of heat was that La³⁺ ion damages the outer cell membrane and increases its permeability and the proton-electron potential energy across the cell membrane was reduced or couldn’t even be initiated. Energy could not be translated into ATP effectively in the course of oxidative phosphorylation resulting in heat release. So, the growth of the cells was inhibited due to scarceness of energy ATP.

Issue Date: 05 March 2008

Cite this article:

LIU Guosheng,LU Yan,RAN Zhilin, et al. Study on eruption of heat for B aroused by lanthanum nitrate and its mechanism[J]. Front. Chem. China, 2008, 3(1): 70-75.

URL:

https://academic.hep.com.cn/fcc/EN/10.1007/s11458-008-0010-7
https://academic.hep.com.cn/fcc/EN/Y2008/V3/I1/70

1	Wadsö I Isothermalmicrocalorimetry in applied biologyThermochimcaActa 2002 394305311
2	Rehak N N Young D S Prospective applications ofcalorimetry in the clinical laboratoryClinicalChemistry 1978 2414141419
3	Giron D J Applicationsof thermal analysis and coupled techniques in pharmaceutical industryJournal of Thermal Analysis and Calorimetry 2002 68335357
4	Wadsö I Robert N G Standards in isothermal MicrocalorimetryPure and Applied Chemistry 2001 7316251639
5	Xie C L Tang H K Song Z H Microcalorimetric study of bacterial growthThermochimca Acta 1988 1233341
6	Li M J Chen X D Liu G S Yang Y Wang X J Geng J Mei Y J Liu Y Shen P Wang J J Analysis of aprE gene expression employingmicrocalorimetric technique and molecular biological methodActa Chimica Sinica 2005 63(18)16461650(in Chinese)
7	Zhu J Y Liu Y Hu Y H Zeng C Zhang L X Cui C Z Huang Y P Shen P Microcalorimetric study on deletion mutagenesis of thegene promoter sequences from the extremely halophilic archaeaActa Chim Sinica 2006 64(6)508514(in Chinese)
8	Ni J Z RareEarth Bioinnorgnic ChemistryBeijingScience Press 1995 1358, 299–311(in Chinese)
9	Zhu B Zhao D Q Ni J Z Cleavage of nucleic acids by lanthanides and their complexesProgress in Chemistry 1998 10(4)395404(in Chinese)
10	Xie C L Sun D U Song Z H Qu S S Liao Y T Liu H S Athermokinetic study of bacterial metabolismThermochimica Acta 1989 142211217
11	Liu G S Li M J Chen X D Liu Y Zhu J C Shen P Calorimetricstudy of the metabolic activity of Escherichia coli B infected byT4 phage in restricted mediumThermochimicaActa 2005 4353437
12	Shen P Experimentof MicrobiologyBeijingHigher Education Press 1999 192205(in Chinese)
13	Liu G S Liu Y Chen X D Liu P Shen P Qu S S Studyon interaction between T4 phage and Escherichia coli B by microcalorimetricmethodJournal of Viroogical Methods 2003 112137143
14	Xie C L Sun D Y Song Z H Qu S S Thermochemicalstudies on cell metabolism. Part I. thermal equations for the metabolismof resting cellsThermochimica Acta 1990 158187191
15	Lacaz-Vieira F Marques M M J Membr Biol 2004 20239
16	Yermiyahu U Rytwo G Brauer D K Kinraide T B Binding andelectrostatic attraction of lanthanum (La³⁺) and aluminum (Al³⁺) to wheat root plasmamembranesJournal of Membrane Biology 1997 159239252
17	Feng Z H Wang X Zhang S X An L Z Zhang J X Yao H Y Astudy of the biological effects of rare earth elements at cellularlevel using nuclear techniquesChinese Journalof Nuclear Mdeicine 2001 21(4)111114(in Chinese)
18	Liu P Liu Y Lu Z X Zhu J C Dong J X Pang D W Shen P Qu S S Study on biological effect of La³⁺ on Escherichia coli by atomic force microscopyJournal of Inorganic Biochemistry 2004 986872
19	Elna P Moore G R Structural characteristicsof protein binding sites for calcium and lanthanide ionsJournal of Biological Inorganic Biochemistry 2001 6479489

Viewed

Full text

Abstract

Cited

Shared

Discussed