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Effect of fatty acid methyl esters from plastrum
testudinis on proliferation of rat bone mesenchymal stem cells |
| ZHANG Yuehua1, ZENG Heping2, CHEN Dongfeng3 |
| 1.Department of Biological and Environmental Engineering, Guangzhou City Polytechnic; 2.Institute of the Functional Molecule, Department of Chemistry, South China University of Technology; 3.Guangzhou University of Traditional Chinese Medicine |
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Abstract The ointment of plastrum testudinis was extracted using petroleum ether, ether and dichloromethane sequentially and the extracts were methyl-esterified. The effects on the proliferation of bone marrow mesenchymal stem cells (bMMSCs) were examined by MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay and flow cytometry analysis. The volatile components of the samples were studied by gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC). The results show that the methyl-esterified parts can promote the proliferation of stem cells and they all contain palmitic acid methyl ester. Palmitic acid methyl ester can promote proliferation when the concentration was 0.15 ?g/?L. It may be concluded that the palmitic acid methyl ester is important for the methyl-esterified parts that have effects on proliferation.
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Issue Date: 05 September 2008
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| 1 |
Zhou J H Chen D F Li H Du S H Li Y W Deng R D Zhang S X Effect of serum containingcarapax et plasturm testudinis on in-vitro proliferation of rat mesenchymalstem cellsJournal of Guangzhou Unirversityof Traditional Chinese Medicine 2005 22(1)3538(in Chinese)
|
| 2 |
Chen D F Du S H Li Y W Zhang J Li H Effect of tortoise shell on expression of nestin followingfocal cerebral ischemia reperfusionChineseJournal of Anatomy 2002 25(4)315318(in Chinese)
|
| 3 |
Chen D F Du S H Li Y W Zhang J Li H Effect of plastrum testudinis on neural stem cell afterfocal cerebral ischemiaJournal of GuangzhouUnirversity of Traditional Chinese Medicine 2001 18(4)328331(in Chinese)
|
| 4 |
Li Y W Chui X J Chen D F Du S H Li Hui Zhou J H Effectof tortoises shell on neural stem cell following injuried spinal cordChinese Journal of Neuroanatomy 2003 19(3)321324(in Chinese)
|
| 5 |
Du S H Chen D F Li Y W Li H Zhou J H Deng R D Zhang S X Effect of plastrum testudinison rats mesenchymal stem cells differentiation into neurons aftercerebral ischemiaNational Medical Journalof China 2005 85(3)205207(in Chinese)
|
| 6 |
Chen D F Du S H Li Y W Li H In-vitro inductionof mesenchymal stem cells differentiation into neurons in adult ratsby serum containing carapax at plastrum testudinisJournal of Guangzhou Unirversity of Traditional Chinese Medicine 2003 20(3)224226(in Chinese)
|
| 7 |
Li H Zhou J H Chen D F Du S H Li Y W Deng R D Zhang S X Effect of plastrum testudinison osteogenesis of cultured rat mesenchymal stem cellsTraditional Chinese Drug Research & Clinical Pharmacology 2005 16(3)159161(in Chinese)
|
| 8 |
Sun S Y Li F M The analysis of fatty acidsin tortoise plastron by GC-MSChinese Journalof Pharmaceutical Analysis 1999 19(6)406409(in Chinese)
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