Thermodynamic analysis of fluorescence enhancement
and Quenching theory equations

doi:10.1007/s11458-008-0065-5

Front. Chem. China

2008, Vol. 3

Issue (3) : 254-261 https://doi.org/10.1007/s11458-008-0065-5

Thermodynamic analysis of fluorescence enhancement and Quenching theory equations

YANG Manman, XI Xiaoli, YANG Pin

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of China at Institute of Molecular Science, College of Chemistry and Chemical Engineering, Shanxi University

Download: PDF(287 KB) HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

Abstract The action of the three kinds of new third generation cephalosporin-class drugs, cefepime hydrochroride, cefpiramide and ceftizoxime with HSA and BSA was studied at different temperatures through the fluorescence method. First, the binding constants were calculated by using fluorescence quenching and enhancement theoretical equations. Their thermodynamic functions were also calculated. Because the K_A corresponding to the different theoretical equations are not completely the same, the thermodynamic parameters calculated are also different. In this paper, the differences among these thermodynamic data obtained from the different theoretical equations were analyzed and the results show that the thermodynamic data deduced from fluorescence enhancement are more reasonable. Thus, we propose that even when the fluorescence quenching action of the acceptor-substrate is studied, more realistic data can be obtained by using the fluorescence enhancement equation.

Issue Date: 05 September 2008

Cite this article:

XI Xiaoli,YANG Manman,YANG Pin. Thermodynamic analysis of fluorescence enhancement and Quenching theory equations[J]. Front. Chem. China, 2008, 3(3): 254-261.

URL:

https://academic.hep.com.cn/fcc/EN/10.1007/s11458-008-0065-5
https://academic.hep.com.cn/fcc/EN/Y2008/V3/I3/254

1	Yang M M Xi X L Yang P The equal- efficiency - proving of fluorescence quenchingand enhancement equationChin Sci Bull 2005 5025712574. doi:10.1360/982005‐499
2	Yang P Yang M M Yang B S The fluorescence enhancement and the action between donrand acceptorChinese Journal of Chemistry 1996 14(2)109113
3	Lakowicz J R Weber G Quenching of fluorescence byoxygenProbe for structural fluctuationsin macromolecules, Biochemistry 1973 12(21)41614170
4	He Y G Newlyapplied pharmacologyBeijingBeijing Science Technology Press 2006 1323
5	Chatelier R C Sawyer W H The transverse organisationof ubiquinones in mitochondrial membranes as determined by fluorescencequenchingEur Biophys J 1985 11(3)179185. doi:10.1007/BF00257396
6	CHEN Guo-Zhen HUANG Xian-Zhi XU Jin-Gou et al.Fluorescence analytical method(2nd Ed.) BeijingScience Press 1990 112116
7	Yang M M Yang P Zhang L W Study on interaction of caffeic acid series medicine andalburmin by fluorescence methodChin SciBull 1994 39(9)734739
8	Ross P D Subramanian S Thermodynamics of protein associationreactions: forces contributing to stabilityBiochemistry 1981 20(11)30963102. doi:10.1021/bi00514a017
9	Lakowicz J R Principlesof fluorescence spectroscopyNew YorkPlenum Press 1999 367394
10	Lakowicz J R Principlesof fluorescence spectroscopyNew YorkPlenum Press 1999 445486
11	Stryer L Hauglandt R P Energy transfer: a spectroscopicruler*Proc Natl Acad Sci USA 1967 58(2)719726. doi:10.1073/pnas.58.2.719
12	Yang P Gao F Principle of bioinorganic chemistryBeijingSciencePress 2002 322342489494(inChinese)
13	Ware W R Oxygenquenchixg of fluorescence in solution: ax experimental study of thediffusion processJ. Phys. Chem. 1962 66(3)455458(A0612076 LU Y. J. LING J.). doi: 10.1021/j100809a020

Viewed

Full text

Abstract

Cited

Shared

Discussed