Conjugated polyelectrolyte amplified fluorescent
assays with probe functionalized silica nanoparticles for chemical
and biological sensing

doi:10.1007/s11458-009-0101-0

Front. Chem. China

2009, Vol. 4

Issue (4) : 325-334 https://doi.org/10.1007/s11458-009-0101-0

Research articles

Conjugated polyelectrolyte amplified fluorescent assays with probe functionalized silica nanoparticles for chemical and biological sensing

Yanyan WANG,Bin LIU,

Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 117576, Singapore;

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Abstract Low-costsensors with high sensitivity and selectivity for chemical and biological detection are of high scientific and economic importance. Silica nanoparticles (NPs) have shown vast promise in sensor applications by virtue of their controllable surface modification, good chemical stability, and biocompatibility. This mini-review summarizes our recent development of silica NP-based assays for chemical and biological detection, where silica NPs serve as the substrate for probe immobilization, target recognition, and separation. The assay performance is further improved through the introduction of conjugated polyelectrolyte to amplify the detection signal. The assays have been demonstrated to be successful for the detection of DNA, small molecules, and proteins. They could be generalized for other targets based on specific interactions, such as DNA hybridization, antibody-antigen recognition, and target-aptamer binding.

Keywords sensor silica nanoparticle conjugated polyelectrolyte energy transfer DNA protein

Issue Date: 05 December 2009

Cite this article:

Yanyan WANG,Bin LIU. Conjugated polyelectrolyte amplified fluorescent assays with probe functionalized silica nanoparticles for chemical and biological sensing[J]. Front. Chem. China, 2009, 4(4): 325-334.

URL:

https://academic.hep.com.cn/fcc/EN/10.1007/s11458-009-0101-0
https://academic.hep.com.cn/fcc/EN/Y2009/V4/I4/325

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