The effect of carboxymethyl-chitosan nanoparticles on proliferation of keloid fibroblast

doi:10.1007/s11458-011-0229-6

Front Chem Chin

2011, Vol. 6

Issue (1) : 31-37 https://doi.org/10.1007/s11458-011-0229-6

RESEARCH ARTICLE

The effect of carboxymethyl-chitosan nanoparticles on proliferation of keloid fibroblast

Chao FENG¹, Xiguang CHEN¹(

), Jing ZHANG¹, Gangzheng SUN¹, Xiaojie CHENG¹, Zhiguo WANG², Hyun-Jin PARK³

1. College of Marine Life Science, Ocean University of China, Qingdao 266003, China; 2. The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China; 3. The Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea

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Abstract

In this study, different molecular weight (MW) carboxymethyl chitosans (CM-chitosan) nanoparticles were prepared by ionic gelification. The particle size of nanoparticles was around 180–250 nm by dynamic light scattering (DLS) and transmission electron microscope (TEM). With the increase of CM-chitosan nanoparticles concentration from 2 to 200 μg/mL, the growth inhibition effects on the keloid fibroblast increased. At the concentration of 100 μg/mL, CM-chitosan nanoparticles with MW 6.3 kDa had a significant inhibitory effect (inhibition ratio 48.79%) of the proliferation of keloid fibroblast. Compared with CM-chitosan solution, the inhibition of CM-chitosan nanoparticles were lower in prior period and similar in later period. By analyzing the different effects of chitosan, CM-chitosan solution and CM-chitosan nanoparticles on proliferation of keloid fibroblast, we have found that the carboxylmethyl groups of CM-chitosan play an important role in inhibition of proliferation of keloid fibroblast.

Keywords carboxymethyl-chitosan nanoparticle keloid fibroblast proliferation

Corresponding Author(s): CHEN Xiguang,Email:xgchen@ouc.edu.cn

Issue Date: 05 March 2011

Cite this article:

Chao FENG,Xiguang CHEN,Jing ZHANG, et al. The effect of carboxymethyl-chitosan nanoparticles on proliferation of keloid fibroblast[J]. Front Chem Chin, 2011, 6(1): 31-37.

URL:

https://academic.hep.com.cn/fcc/EN/10.1007/s11458-011-0229-6
https://academic.hep.com.cn/fcc/EN/Y2011/V6/I1/31

Tab.1 Reaction solvents, typical mean particle sizes and polydispersity index (PI) of CM-chitosan nanoparticles samples ( = 3)

Fig.1 Particle size distribution of CM-chitosan nanoparticles with different MWs ( = 3) (a) 196 kDa; (b) 16 kDa; (c) 7.5 kDa; (d) 6.3 kDa

Fig.2 TEM images of CM-chitosan nanoparticles with different MWs (a) CM-chitosan nanoparticles 196 kDa; (b) CM-chitosan nanoparticles 16 kDa; (c) CM-chitosan nanoparticles 7.5 kDa; (d) CM-chitosan nanoparticles 6.3 kDa

Fig.3 Effects of concentration of CM-chitosan nanoparticles with different MWs on the proliferation of the keloid fibroblast. Cell culture was in media of DMEM contained 10% BCS, 96-well tissue culture plate; initial cell number was 5 × 10 cells per well; 5% CO and 37°C. Cell numbers were determined after four days of incubation by MTT method ( = 6). Control group contained 40 μg/mL CaCl. Cell count= (OD of test) / (OD of control)

Fig.4 Effects of exposure time of CM-chitosan nanoparticles with different MWs on the proliferation of the keloid fibroblast. Cell culture was in media of DMEM contained 10% BCS, 96-well tissue culture plate; initial cell number was 4 × 10 cells per well, 5% CO and 37°C.Control group contained 40 μg/mL CaCl. Cell count= (OD of test) / (OD of control). Test Groups contained 100 μg/mL CM-chitosan nanoparticles ( = 6)

Fig.5 Effects of CM-chitosan nanoparticles and CM-chitosan solution on the proliferation of the keloid fibroblast. (I) MW 196 kDa; (II) MW 16 kDa; (III) MW 7.5 kDa. Cell numbers were determined after four days of incubation by MTT method ( = 6). Cell culture was in media of DMEM contained 10% BCS, 96-well tissue culture plate; initial cell number was 6 × 10 cells per well; 5% CO and 37°C.Control group contained 40 μg/ml CaCl. Test Groups contained 100 μg/mL CM-chitosan solution or CM-chitosan nanoparticles.

Fig.6 Effects of chitosan, CM-chitosan solution and CM-chitosan nanoparticles on the proliferation of the keloid fibroblast after specific periods (0 d, 1 d, 2 d, 4 d, 6 d) of incubation. Cell culture was in media of DMEM contained 10% BCS, 96-well tissue culture plate, initial cell number was 5 × 10 cells per well, 5% CO and 37°C. Control group contained 40 μg/mL CaCl. Cell count= (OD of test) / (OD of control). Test Groups contained 100 μg/mL chitosan CM-chitosan solution or CM-chitosan nanoparticles ( = 6)

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