Preparation of D-lysine by chemical reaction
and microbial asymmetric transformation
Preparation of D-lysine by chemical reaction
and microbial asymmetric transformation
LIU Yi1, JIAO Qingcai2, YIN Xiaoxing3
1.Department of Pharmacy, Xuzhou Medical College; State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University; 2.State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University; 3.Department of Pharmacy, Xuzhou Medical College
Abstract：The DL-lysine crystals from the racemization of L-lysine was treated as substrate with Hafnia alvei AS1.1009 intact cells as biocatalysts to produce crystalline D-lysine with a yield of 56.6% from the reaction mixture after simple purification. In the presence of 0.10 molar equivalent of salicylaldehyde, L-lysine racemization can be completed within 4 h in 1.0 mol/L of NaOH at 100°C. The activation energy of the processes was 62187.86 J/mol. The characteristics of Hafnia alvei AS1.1009 decarboxylase were studied. Under the conditions of pH 8.0, temperature 37°C, cell concentration 10 g/L, tween-80 0.5 g/L, substrate concentration 30 g/L, and the specific activity of up to 3840 U, L-lysine can be completely degraded by the decarboxylase for 12 h under the optimal conditions.
出版日期: 2008-03-05
引用本文:
. Preparation of D-lysine by chemical reaction
and microbial asymmetric transformation[J]. Frontiers of Chemical Engineering in China - Selected Publications from Chinese Universities, 2008, 2(1): 40-43.
LIU Yi, JIAO Qingcai, YIN Xiaoxing. Preparation of D-lysine by chemical reaction
and microbial asymmetric transformation. Front. Chem. Sci. Eng., 2008, 2(1): 40-43.
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