Abstract：A reliable and accurate liquid chromatography with evaporative light scattering detection (LC-ELSD) method was developed for simultaneous determination of five constituents in Xue-Fu-Zhu-Yu capsule, a widely used prescription of traditional Chinese medicine (TCM). The chromatographic separation was performed on a Symmetry C18 column (150mm×4.6mm, 5.0μm) using gradient elution of acetonitrile and water (containing 1.0% acetic acid). The flow rate was 0.6mL/min and the temperature of column was 30°C. A good linearity correlation was obtained over the investigated concentration ranges. Recoveries of the five components varied from 97.31% to 99.84% (RSD<4.0%). The validated method was successfully applied to the quality assessment of Xue-Fu-Zhu-Yu capsule.
出版日期: 2010-03-05
引用本文:
. LC-ELSD analysis for simultaneous determination
of five components in the Xue-Fu-Zhu-Yu capsules[J]. Front. Chem. Sci. Eng., 2010, 4(1): 96-101.
Ying GAO, Wenyuan GAO, Xuan DONG, Fengge LI, Xinying YANG, . LC-ELSD analysis for simultaneous determination
of five components in the Xue-Fu-Zhu-Yu capsules. Front. Chem. Sci. Eng., 2010, 4(1): 96-101.
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