Abstract:Thrombus formation and blood coagulation are serious problems associated with blood contacting products, such as catheters, vascular grafts, artificial hearts, and heart valves. Recent progresses and strategies to improve the hemocompatibility of biomaterials by surface modification using photochemical immobilization and photograft polymerization are reviewed in this paper. Three approaches to modify biomaterial surfaces for improving the hemocompatibility, i.e., bioinert surfaces, immobilization of anticoagulative substances and biomimetic surfaces, are introduced. The biomimetic amphiphilic phosphorylcholine and Arg-Gly-Asp (RGD) sequence are the most effective and most often employed biomolecules and peptide sequence for improving hemocompatibility of material surfaces. The RGD sequence can enhance adhesion and growth of endothelial cells (ECs) on material surfaces and increase the retention of ECs under flow shear stress conditions. This surface modification is a promising strategy for biomaterials especially for cardiovascular grafts and functional tissue engineered blood vessels.
出版日期: 2010-09-05
引用本文:
. Surface modification of biomaterials by photochemical
immobilization and photograft polymerization to improve hemocompatibility[J]. Front. Chem. Sci. Eng., 2010, 4(3): 372-381.
Yakai FENG, Haiyang ZHAO, Li ZHANG, Jintang GUO, . Surface modification of biomaterials by photochemical
immobilization and photograft polymerization to improve hemocompatibility. Front. Chem. Sci. Eng., 2010, 4(3): 372-381.
Lee J H, Lee H B, Andrade J D. Blood compatibility of polyethylene oxidesurfaces. Progress in Polymer Science, 1995, 20(6): 1043―1079 doi: 10.1016/0079-6700(95)00011-4
Sakuragi M, Tsuzuki S, Hasuda H, Wada A, Matoba K, Kubo I, Ito Y. Synthesis of a photoimmobilizable histidine polymer forsurface modification. Journal of AppliedPolymer Science, 2009, 112(1): 315―319 doi: 10.1002/app.29427
Lamponi S, Di Canio C, Forbicioni M, Barbucci R. Heterotypic interaction of fibroblasts and endothelialcells on restricted area. Journal of BiomedicalMaterials Research, 2010, 92A(2): 733―745 doi: 10.1002/jbm.a.32364
Bhattacharya A, Misra B N. Grafting:a versatile means to modify polymers—techniques, factors andapplications. Progress in Polymer Science, 2004, 29(8): 767―814 doi: 10.1016/j.progpolymsci.2004.05.002
He D M, Susanto H, Ulbricht M. Photo-irradiation for preparation,modification and stimulation of polymeric membranes. Progress in Polymer Science, 2009, 34(1): 62―98 doi: 10.1016/j.progpolymsci.2008.08.004
Susanto H, Ulbricht M. Photograftedthin polymer hydrogel layers on PES ultrafiltration membranes: characterization,stability, and influence on separation performance. Langmuir, 2007, 23(14): 7818―7830 doi: 10.1021/la700579x
Yu Y B, Liu L Y, Sun Y F, He C F, Yang W T. Studies on photograftingof triemethylol propane triacrylate onto the surface of LDPE films. Acta Polymerica Sinica, 2006(3): 455―460
Janorkar A V, Proulx S E, Metters A T, Hirt D E. Surface-confined photopolymerization of single- and mixed-monomersystems to tailor the wettability of poly(L-lactide) film. Journal of Polymer Science. Part A, Polymer Chemistry, 2006, 44(22): 6534―6543 doi: 10.1002/pola.21700
Zhu S Q, Hirt D E. Improvingthe wettability of deep-groove polypropylene fibers by photografting. Textile Research Journal, 2009, 79(6): 534―547 doi: 10.1177/0040517508092017
Suri S, Singh A, Schmidt C E. Photofunctionalization of materials topromote protein and cell interactions for tissue-engineering applications. In: Puleo D A, Bizios R, eds. Biological Interactions on Materials Surfaces. New York: Springer, 2009, 297―318 doi: 10.1007/978-0-387-98161-1_15
Fax?lv L, Ekblad T, Liedberg B, Lindahl T L. Blood compatibility of photografted hydrogel coatings. Acta Biomaterialia, 2010
Yamada K, Takeda S, Hirata M. Improvement of autohesiveand adhesive properties of polyethylene plates by photografting withglycidyl methacrylate. Journal of AppliedPolymer Science, 2007, 103(1): 493―500 doi: 10.1002/app.25076
Gutierrez-Villarreal M H, Ulloa-Hinojosa M G, Gaona-Lozano J G. Surface functionalization of poly(lactic acid) film by UV-photograftingof N-vinylpyrrolidone. Journal of Applied Polymer Science, 2008, 110(1): 163―169 doi: 10.1002/app.28000
Kyomoto M, Moro T, Takatori Y, Kawaguchi H, Nakamura K, Ishihara K. Self-initiated surface grafting with poly(2-methacryloyloxyethylphosphorylcholine) on poly(ether-ether-ketone). Biomaterials, 2010, 31(6): 1017―1024 doi: 10.1016/j.biomaterials.2009.10.055
Dai Q W, Xu Z K, Wu J. A novel approach for the surface modification of polymericmembrane with phospholipid polymer. ChineseChemical Letters, 2004, 15(8): 993―996
Bae J W, Choi J H, Kim T E, Park K D, Kim J Y, Park Y D, Sun K. Heparinizedmicropatterned surfaces for the spatial control of human mesenchymalstem cells. Journal of Bioactive and CompatiblePolymers, 2009, 24(6): 493―506 doi: 10.1177/0883911509349143
Lin W C, Yu D G, Yang M C. Blood compatibility of thermoplasticpolyurethane membrane immobilized with water-soluble chitosan/dextransulfate. Colloid Surface B: Biointerfaces, 2005, 44(2―3): 82―92
Nagase K, Kobayashi J, Pkano T. Temperature-responsive intelligentinterfaces for biomolecular separation and cell sheet engineering. Journal of the Royal Society, Interface, 2009, 6(3 Suppl_3): S293―S309
Guan J J, Gao C, Feng L X, Sheng J C. Surface photo-grafting of polyurethane with 2-hydroxyethyl acrylatefor promotion of human endothelial cell adhesion and growth. Journal of Biomaterials Science. Polymer Edition, 2000, 11(5): 523―536 doi: 10.1163/156856200743841
Wang Y J, Ke Y, Ren L, Wu G, Chen X F, Zhao Q C. Surface engineering of PHBV by covalent collagen immobilizationto improve cell compatibility. Journalof Biomedical Materials Research. Part A, 2009, 88A(3): 616―627 doi: 10.1002/jbm.a.31858
Rana D, Matsuura T. Surfacemodifications for antifouling membranes. Chemical Reviews, 2010, doi:10.1021/cr800208y doi: 10.1021/cr800208y
Dai L, Mau A W H. Surfaceand interface control of polymeric biomaterials, conjugated polymers,and carbon nanotubes. Journal of PhysicalChemistry B, 2000, 104(9): 1891―1915
Altankov G, Thom V, Groth T, Jankova K, Jonsson G, Ulbricht M. Modulating the biocompatibility of polymer surfaces withpoly(ethylene glycol): effect of fibronectin. Journal of Biomedical Materials Research, 2000, 52(1): 219―230 doi: 10.1002/1097-4636(200010)52:1<219::AID-JBM28>3.0.CO;2-F
Chen H, Yuan L, Song W, Wu Z, Li D. Biocompatible polymer materials:role of protein-surface interactions. Progressin Polymer Science, 2008, 33(11): 1059―1087 doi: 10.1016/j.progpolymsci.2008.07.006
Iguerb O, Bertrand P. Graftphotopolymerization of polyethylene glycol monoacrylate (PEGA) onpoly(methyl methacrylate) (PMMA) films to prevent BSA adsorption. Surface and Interface Analysis, 2008, 40(3―4): 386―390
Joung Y K, Choi J H, Bae J W, Park K D. Hyper-branched poly(poly(ethylene glycol)methacrylate)-grafted surfacesby photo-polymerization with iniferter for bioactive interfaces. Acta Biomaterialia, 2008, 4(4): 960―966 doi: 10.1016/j.actbio.2008.02.008
Sebra R P, Reddy S K, Masters K S, Bowman C N, Anseth K S. Controlled polymerizationchemistry to graft architectures that influence cell-material interactions. Acta Biomaterialia, 2007, 3(2): 151―161 doi: 10.1016/j.actbio.2006.07.010
Stachowiak T B, Svec F, Frechet J. Patternable protein resistant surfacesfor multifunctional microfluidic devices via surface hydrophilizationof porous polymer monoliths using photografting. Chemistry of Materials, 2006, 18(25): 5950―5957 doi: 10.1021/cm0617034
Francois P, Vaudaux P, Nurdin N, Mathieu H J, Descouts P, Lew D P. Physical and biological effectsof a surface coating procedure on polyurethane catheters. Biomaterials, 1996, 17(7): 667―678 doi: 10.1016/0142-9612(96)86736-6
Wetzels G M R, Koole L H. Photoimmobilisationof poly(N-vinylpyrrolidinone) asa means to improve haemocompatibility of polyurethane biomaterials. Biomaterials, 1999, 20(20): 1879―1887
Mao C, Zhang C, Qiu Y Z, Zhu A P, Shen J, Lin S C. Introduction of anticoagulation group to polypropylenefilm by radiation grafting and its blood compatibility. Applied Surface Science, 2004, 228(1―4): 26―33
Mao C, Zhao W B, Zhu A P, Shen J, Lin S C. A photochemical method forthe surface modification of poly(vinyl chloride) with O-butyrylchitosan to improve blood compatibility. Process Biochemistry (Barking, London, England), 2004, 39(9): 1151―1157 doi: 10.1016/S0032-9592(03)00225-5
Mao C, Zhao W B, Zhu C H, Zhu A P, Shen J, Lin S C. In vitro studies of platelet adhesion on UV radiation-treatednylon surface. Carbohydrate Polymers, 2005, 59(1): 19―25 doi: 10.1016/j.carbpol.2004.08.016
Bhat V T, James N R, Jayakrishnan A. A photochemical method forimmobilization of azidated dextran onto aminated poly(ethylene terephthalate)surfaces. Polymer International, 2008, 57(1): 124―132 doi: 10.1002/pi.2332
Ravi S, Chaikof E L. Biomaterials for vascular tissue engineering. Regenerative Medicine, 2010, 5(1): 107―120 doi: 10.2217/rme.09.77
Aldenhoff Y B, Blezer R, Lindhout T, Koole L H. Photo-immobilization of dipyridamole (Persantin) at thesurface of polyurethane biomaterials: reduction of in-vitro thrombogenicity. Biomaterials, 1997, 18(2): 167―172 doi: 10.1016/S0142-9612(96)00095-6
Zhu A P, Ming Z, Jian S. Blood compatibility of chitosan/heparincomplex surface modified ePTFE vascular graft. Applied Surface Science, 2005, 241(3―4): 485―492
Zhao G W, Chen Y S, Dong T, Wang X L. Surface modification of polyethylene by heparin for improvement ofantithrombogenicity. Plasma Science andTechnology, 2007, 9(2): 202―205 doi: 10.1088/1009-0630/9/2/18
Li D, Chen H, Glenn McClung W, Brash J L. Lysine-PEG-modified polyurethane as a fibrinolytic surface:Effect of PEG chain length on protein interactions, platelet interactionsand clot lysis. Acta Biomaterialia, 2009, 5(6): 1864―1871
Chen H, Zhang Y, Li D, Hu X Y, Wang L, McClung W G, Brash J L. Surfaces having dual fibrinolyticand protein resistant properties by immobilization of lysine on polyurethanethrough a PEG spacer. Journal of BiomedicalMaterials Research, 2009, 90A(3): 940―946 doi: 10.1002/jbm.a.32152
McClung W G, Babcock D E, Brash J L. Fibrinolytic properties oflysine-derivatized polyethylene in contact with flowing whole blood(Chandler Loop model). Journal of BiomedicalMaterials Research. Part A, 2007, 81A(3): 644―651 doi: 10.1002/jbm.a.31018
McClung W G, Clapper D L, Anderson A B, Babcock D E, Brash J L. Interactionsof fibrinolytic system proteins with lysine containing surfaces. Journal of Biomedical Materials Research. PartA, 2003, 66A(4): 795―801 doi: 10.1002/jbm.a.10017
Lewis A L. Phosphorylcholine-based polymers and their use in theprevention of biofouling. Colloids andSurfaces. B, Biointerfaces, 2000, 18(3―4): 261―275 doi: 10.1016/S0927-7765(99)00152-6
Ishihara K, Tsuino R, Hamada M, Toyoda N, Iwasaki Y. Stabilizedliposomes with phospholipid polymers and their interactions with bloodcells. Colloids and Surfaces. B, Biointerfaces, 2002, 25(4): 325―333 doi: 10.1016/S0927-7765(02)00006-1
Huangfu P B, Gong M, Zhang C, Yang S, Zhao J, Gong Y K. Cell outer membrane mimetic modification of a cross-linkedchitosan surface to improve its hemocompatibility. Colloids and Surfaces. B, Biointerfaces, 2009, 71(2): 268―274 doi: 10.1016/j.colsurfb.2009.02.014
Seo J H, Matsuno R, Takai M, Ishihara K. Cell adhesion on phase-separated surface of block copolymercomposed of poly(2-methacryloyloxyethyl phosphorylcholine) and poly(dimethylsiloxane). Biomaterials, 2009, 30(29): 5330―5340 doi: 10.1016/j.biomaterials.2009.06.031
Xu Y, Takai M, Ishihara K. Protein adsorption and cell adhesionon cationic, neutral, and anionic 2-methacryloyloxyethyl phosphorylcholinecopolymer surfaces. Biomaterials, 2009, 30(28): 4930―4938 doi: 10.1016/j.biomaterials.2009.06.005
Hoven V P, Chombanpaew K, Iwasaki Y, Tasakorn P. Improving blood compatibility of natural rubber by UV-inducedgraft polymerization of hydrophilic monomers. Journal of Applied Polymer Science, 2009, 112(1): 208―217 doi: 10.1002/app.29408
Sawada S, Sakaki S, Iwasaki Y, Nakabayashi N, Ishihara K. Suppressionof the inflammatory response from adherent cells on phospholipid polymers. Journal of Biomedical Materials Research. PartA, 2003, 64A(3): 411―416 doi: 10.1002/jbm.a.10433
Patel J D, Iwasaki Y, Ishihara K, Anderson J M. Phospholipid polymer surfaces reduce bacteria and leukocyteadhesion under dynamic flow conditions. Journal of Biomedical Materials Research. Part A, 2005, 73A(3): 359―366 doi: 10.1002/jbm.a.30302
Sawada S I, Iwasaki Y, Nakabayashi N, Ishihara K. Stress response of adherent cells on a polymer blendsurface composed of a segmented polyurethane and MPC copolymers. Journal of Biomedical Materials Research. PartA, 2006, 79A(3): 476―484 doi: 10.1002/jbm.a.30820
Matsuda Y, Kobayashi M, Annaka M, Ishihara K, Takahara A. Dimensionof poly(2-methacryloyloxyethyl phosphorylcholine) in aqueous solutionswith various ionic strength. ChemistryLetters, 2006, 35(11): 1310―1311 doi: 10.1246/cl.2006.1310
Lam J K W, Ma Y, Armes S P, Lewis A L, Baldwin T, Stolnik S. Phosphorylcholine-polycation diblock copolymers as syntheticvectors for gene delivery. Journal of ControlledRelease, 2004, 100(2): 293―312 doi: 10.1016/j.jconrel.2004.08.028
Chen M, Briscoe W H, Armes S P, Klein J. Lubrication at physiological pressures by polyzwitterionicbrushes. Science, 2009, 323(5922): 1698―1701 doi: 10.1126/science.1169399
Van der Heiden A P, Goebbels D, Pijpers A P, Koole L H. A photochemical method for the surface modification ofpoly(etherurethanes) with phosphorylcholine-containing compounds toimprove hemocompatibility. Journal of BiomedicalMaterials Research. Part A, 1997, 37A(2): 282―290 doi: 10.1002/(SICI)1097-4636(199711)37:2<282::AID-JBM19>3.0.CO;2-G
Konno T, Hasuda H, Ishihara K, Ito Y. Photo-immobilization of a phospholipid polymer for surfacemodification. Biomaterials, 2005, 26(12): 1381―1388 doi: 10.1016/j.biomaterials.2004.04.047
Goda T, Matsuno R, Konno T, Takai M, Ishihara K. Photograftingof 2-methacryloyloxyethyl phosphorylcholine from polydimethylsiloxane:tunable protein repellency and lubrication property. Colloids and Surfaces. B, Biointerfaces, 2008, 63(1): 64―72 doi: 10.1016/j.colsurfb.2007.11.014
Herring M B, Gardner A L, Glover J. A single-staged techniquefor seeding vascular grafts with autogenous endothelium. Surgery, 1978, 84(4): 498―504
de Mel A, Jell G, Stevens M M, Seifalian A M. Biofunctionalization of biomaterials for accelerated in situ endothelialization:a review. Biomacromolecules, 2008, 9(11): 2969―2979 doi: 10.1021/bm800681k
Monchaux E, Vermette P. Effectsof surface properties and bioactivation of biomaterials on endothelialcells. Frontiers in Bioscience, 2010, S2(1): 239―255 (Schol Ed) doi: 10.2741/s61
Zhu Y B, Gao C Y, Guan J J, Shen J C. Engineering porous polyurethane scaffolds by photografting polymerizationof methacrylic acid for improved endothelial cell compatibility. Journal of Biomedical Materials Research. PartA, 2003, 67A(4): 1367―1373 doi: 10.1002/jbm.a.20058
Thom V H, Altankov G, Groth Th, Jankova K, Jonsson G, Ulbricht M. Optimizing cell-surface interactionsby photografting of poly(ethylene glycol). Langmuir, 2000, 16(6): 2756―2765 doi: 10.1021/la990303a
Janorkar A V, Fritz Jr E W, Burg K J L, Metters A T, Hirt D E. Graftingamine-terminated branched architectures from poly(l-lactide) filmsurfaces for improved cell attachment. J Biomed Mater Res B, 2007, 81B(1): 142―152 doi: 10.1002/jbm.b.30647
Ohmuro-Matsuyama Y, Tatsu Y. Photocontrolledcell adhesion on a surface functionalized with a caged arginine-glycine-aspartatepeptide. Angewandte Chemie, 2008, 120(39): 7637―7639 doi: 10.1002/ange.200802731
Qin T W, Yang Z M, Wu Z Z, Xie H Q, Qin J, Cai S X. Adhesion strength of human tenocytes to extracellularmatrix component-modified poly(DL-lactide-co-glycolide) substrates. Biomaterials, 2005, 26(33): 6635―6642 doi: 10.1016/j.biomaterials.2005.04.023
Williams S K, Kleinert L B, Hagen K M, Clapper D L. Covalent modification of porous implants using extracellularmatrix proteins to accelerate neovascularization. Journal of Biomedical Materials Research. Part A, 2006, 78A(1): 59―65 doi: 10.1002/jbm.a.30659
Zhang H, Lin C Y, Hollister S J. The interaction between bonemarrow stromal cells and RGD-modified three-dimensional porous polycaprolactonescaffolds. Biomaterials, 2009, 30(25): 4063―4069 doi: 10.1016/j.biomaterials.2009.04.015
Hersel U, Dahmen C, Kessler H. RGD modified polymers: biomaterialsfor stimulated cell adhesion and beyond. Biomaterials, 2003, 24(24): 4385―4415 doi: 10.1016/S0142-9612(03)00343-0
Chung T W, Lu Y F, Wang S S, Lin Y S, Chu S H. Growth of human endothelialcells on photochemically grafted Gly-Arg-Gly-Asp (GRGD) chitosans. Biomaterials, 2002, 23(24): 4803―4809 doi: 10.1016/S0142-9612(02)00231-4
Lin Y S, Wang S S, Chung T W, Wang Y H, Chiou S H, Hsu J J, Chou N K, Hsieh K H, Chu S H. Growth of endothelial cells on different concentrations of Gly-Arg-Gly-Aspphotochemically grafted in polyethylene glycol modified polyurethane. Artificial Organs, 2001, 25(8): 617―621 doi: 10.1046/j.1525-1594.2001.025008617.x
Li J H, Ding M M, Fu Q, Tan H, Xie X Y, Zhong Y P. A novel strategy to graft RGD peptide on biomaterials surfaces forendothelization of small-diamater vascular grafts and tissue engineeringblood vessel. J Mater Sci-mater M, 2008, 19(7): 2595―2603 doi: 10.1007/s10856-007-3354-5