Application of statistical design for the production of inulinase by streptomyces sp. using pressmud
Application of statistical design for the production of inulinase by streptomyces sp. using pressmud
M. DILIPKUMAR1(), M. RAJASIMMAN1, N. RAJAMOHAN2
1. Department of Chemical Engineering, Annamalai University, Annamalainagar-608002, Tamilnadu, India; 2. Department of Chemical Engineering, Sohar University, Sohar, Sultanate of Oman
A Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using streptomyces sp. and pressmud as the substrate via solid-state fermentation (SSF). From the experiments, three nutrients viz. yeast extract, FeSO4·7H2O, and NH4NO3 were found to be the most significant components. Hence these three components were selected and optimized using Response Surface Methodology (RSM). The optimum conditions are: yeast extract 0.00274 g/gds, FeSO4·7H2O 0.00011 g/gds and NH4NO3 0.00772 g/gds. The effect of the substrate concentration and initial moisture content were also studied. A substrate concentration of 12 g and an initial moisture content of 65% are optimum for the maximum production of inulinase (89 U/gds).
. Application of statistical design for the production of inulinase by streptomyces sp. using pressmud[J]. Frontiers of Chemical Science and Engineering, 2011, 5(4): 463-470.
M. DILIPKUMAR, M. RAJASIMMAN, N. RAJAMOHAN. Application of statistical design for the production of inulinase by streptomyces sp. using pressmud. Front Chem Sci Eng, 2011, 5(4): 463-470.
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