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Frontiers of Chemical Science and Engineering

ISSN 2095-0179

ISSN 2095-0187(Online)

CN 11-5981/TQ

邮发代号 80-969

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Frontiers of Chemical Science and Engineering  2016, Vol. 10 Issue (1): 108-119   https://doi.org/10.1007/s11705-016-1553-7
  本期目录
Antioxidant and antitumor effects and immunomodulatory activities of crude and purified polyphenol extract from blueberries
Xiaohong Kou1,Lihua Han1,Xingyuan Li1,2,Zhaohui Xue1,*(),Fengjuan Zhou1,*()
1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
2. Sinograin Oil (Tianjin) Corp., Tianjin 300461, China
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Abstract：

The antioxidant and antitumor effects as well as the immunomodulatory activities of crude and purified polyphenol extract from blueberries were investigated. The antioxidant and antitumor effects of the polyphenol extract were measured both in vitro and in vivo, and their effect on the immune systems of CD-1 tumor-bearing mice were also analyzed. In vitro assays demonstrated that blueberry purified polyphenol extract (BBPP) exhibited higher antioxidant activities than blueberry crude polyphenol extract (BBCP), but the opposite effect was observed in vivo. Both the in vitro and in vivo antitumor activity and the immunity assay showed that BBCP not only inhibited tumor growth, but also significantly improved the immunity of the mice. According to physical and histological studies, the CD-1 tumor-bearing mice treated with the polyphenol extract, especially high doses of BBCP experienced a higher quality of life than the positive control group (treated with cyclophosphamide). These results indicate that BBCP has significant antioxidant and antitumor activities and that it can enhance the immunity of CD-1 tumor-bearing mice.
Key words： crude polyphenol extract    purified polyphenol extract    blueberry    antioxidant    antitumor    immunity
收稿日期: 2015-10-07      出版日期: 2016-02-29
Corresponding Author(s): Zhaohui Xue,Fengjuan Zhou   
 引用本文:   
. [J]. Frontiers of Chemical Science and Engineering, 2016, 10(1): 108-119.
Xiaohong Kou,Lihua Han,Xingyuan Li,Zhaohui Xue,Fengjuan Zhou. Antioxidant and antitumor effects and immunomodulatory activities of crude and purified polyphenol extract from blueberries. Front. Chem. Sci. Eng., 2016, 10(1): 108-119.
 链接本文:  
https://academic.hep.com.cn/fcse/CN/10.1007/s11705-016-1553-7
https://academic.hep.com.cn/fcse/CN/Y2016/V10/I1/108
Groups Dose
NC 0.2 mL saline
TC 0.2 mL saline
PC 20 mg/(kg bw·d) CY, 0.2 mL saline
BBCP-LD 50 mg/(kg·d) BBCP-HD, 0.2 mL saline
BBCP-MD 100 mg/(kg·d) BBCP-MD, 0.2 mL saline
BBCP-HD 150 mg/(kg·d) BBCP-LD, 0.2 mL saline
BBPP-LD 50 mg/(kg·d) BBPP-HD, 0.2 mL saline
BBPP-MD 100 mg/(kg·d) BBPP-MD, 0.2 mL saline
BBPP-HD 150 mg/(kg·d) BBPP-LD, 0.2 mL saline
Tab.1  
Fig.1  
Peak Retention time/min [M – H]– M Indentification
1 4.08 207/M – H+ 208 Uncertain
2 6.26 435/M+ H+ 434 Quercetin-3-O-hexose
3 8.58 463/M+ H+ 462 Quercetin-3-O-galactoside
4 11.12 491/M+ H+ 490 Uncertain
5 12.69 369/M – H+ 370 Mallow-3-arabinoside-5-glucoside
6 86.67 249/M – H+ 250 Uncertain
7 89.52 279/M – H+ 280 Uncertain
8 91.26 279/M – H+ 280 Uncertain
9 106.72 280/M+ 280 Uncertain
10 110.29 282/M+ 282 Uncertain
11 113.98 285/M – H+ 286 Didymin
12 115.24 291/M – H+ 292 Epicatechin
13 118.11 291/M – H+ 292 Epicatechin
Tab.2  
Fig.2  
Group Dose/(mg·kg–1) MDA content in liver/(nmol·mgprot–1) SOD activity in liver/(U·mgprot–1) MDA content in lung/(nmol·mgprot–1) SOD activity in lung/(U·mgprot–1) NO/(mmol·L–1) Spleen index/(mg·g–1) Thymus index/(mg·g–1) Tumor weight/g Weight gain/g DTH/mg Macrophages phagocytic/OD Spleen lymphocyte/OD
NC – 0.91±0.17a) 323.93±20.12a) 1.04±0.07 205.4±10.82 6.7±0.54 30.54±3.66 6.23±0.12 – 6.02±0.45 3.52±0.21 5.87±0.57a) 3.04±0.25a)
TC – 3.19±0.42b) 163.93±11.45b) 4.56±0.33b) 123.1±7.54b) 39.62±4.71b) 17.23±2.54 2.54±0.39 0.74±0.11 2.87±0.31 0.8±0.11 2.69±0.38 0.17±0.03
PC 20 2.54±0.31a,b) 112.46±9.80a,b) 4.23±0.41b) 97.8±5.21b) 27.57±2.33a,b) 17.67±1.85 3.51±0.43a) 0.25±0.04a) –1.25±0.18a) 1.1±0.17 3.03±0.41 0.49±0.09a)
BBCP-LD 50 2.27±0.34a,b) 200.32±9.93a,b) 3.01±0.28a,b) 133.8±6.29b) 19.56±2.03a,b) 22.36±2.61a) 3.71±0.26a) 0.57±0.03a) 2.60±0.37 1.95±0.24a) 3.76±0.42a) 1.38±0.09a)
BBCP-MD 100 1.74±0.27a,b) 230.40±14.71a,b) 2.32±0.31a,b) 152.0±5.47a,b) 16.42±1.28a,b) 23.93±2.17a) 4.58±0.35a) 0.52±0.03a) 3.76±0.42a) 2.62±0.37a) 4.59±0.31a) 1.73±0.31a)
BBCP-HD 150 1.29±0.19a) 265.80±15.28a) 2.00±0.35a,b) 179.0±6.33a,b) 10.92±0.78a,b) 25.37±1.45a) 5.03±0.42a) 0.48±0.06a) 4.87±0.39a) 2.96±0.33a) 5.22±0.28a) 2.58±0.33a)
BBPP-LD 50 2.23±0.35a,b) 198.75±8.79a,b) 3.07±0.41a,b) 117.4±4.37b) 33.66±3.55b) 20.34±1.65a) 3.47±0.33a) 0.66±0.12a) 2.34±0.14 1.75±0.16a) 3.42±0.27a) 1.29±0.12a)
BBPP-MD 100 1.87±0.24a,b) 210.21±15.65a,b) 2.54±0.36a,b) 130.0±3.19a,b) 24.52±2.37a,b) 22.43±2.14a) 3.86±0.28a) 0.61±0.09a) 3.58±0.41a) 1.99±0.22a) 4.06±0.53a) 1.61±0.23a)
BBPP-HD 150 1.65±0.08a,b) 220.95±15.69a,b) 2.20±0.19a,b) 145.0±5.44a,b) 19.47±1.70a,b) 23.25±3.12a) 4.24±0.44a) 0.57±0.04a) 4.12±0.36a) 2.46±0.31a) 4.91±0.64a) 2.26±0.21a)
Tab.3  
Fig.3  
Fig.4  
Fig.5  
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