Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH: A mini review

doi:10.1007/s11705-016-1591-1

Frontiers of Chemical Science and Engineering

2017, Vol. 11

Issue (1): 66-71 https://doi.org/10.1007/s11705-016-1591-1

本期目录

Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH: A mini review

Jongmoon Park^1,^2,³,Yunnam Choi¹(

)

¹. Department of Chemical Engineering, Pohang University of Science and Technology, Gyeongbuk 790-784, Korea
². School of Environmental Science and Engineering, Pohang University of Science and Technology, Gyeongbuk 790-784, Korea
³. Division of Advanced Nuclear Engineering, Pohang University of Science and Technology, Gyeongbuk 790-784, Korea

全文: PDF(141 KB) HTML

文章导读

Abstract：

Cyanobacteria can produce useful renewable fuels and high-value chemicals using sunlight and atmospheric carbon dioxide by photosynthesis. Genetic manipulation has increased the variety of chemicals that cyanobacteria can produce. However, their uniquely abundant NADPH-pool, in other words insufficient supply of NADH, tends to limit their production yields in case of utilizing NADH-dependent enzyme, which is quite common in heterotrophic microbes. To overcome this cofactor imbalance and enhance cyanobacterial fuel and chemical production, various approaches for cofactor engineering have been employed. In this review, we focus on three approaches: (1) utilization of NADPH-dependent enzymes, (2) increasing NADH production, and (3) changing cofactor specificity of NADH-dependent enzymes from NADH to NADPH.

Key words： NADH-dependent enzyme NADPH-dependent enzyme transhydrogenase site-directed mutagenesis enzyme engineering

收稿日期: 2016-06-02 出版日期: 2017-03-17

Corresponding Author(s): Yunnam Choi

引用本文:

. [J]. Frontiers of Chemical Science and Engineering, 2017, 11(1): 66-71.
Jongmoon Park,Yunnam Choi. Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH: A mini review. Front. Chem. Sci. Eng., 2017, 11(1): 66-71.

链接本文:

https://academic.hep.com.cn/fcse/CN/10.1007/s11705-016-1591-1
https://academic.hep.com.cn/fcse/CN/Y2017/V11/I1/66

Tab.1

Fig.1

1	Parmar A, Singh N K, Pandey A, Gnansounou E, Madamwar D. Cyanobacteria and microalgae: A positive prospect for biofuels. Bioresource Technology, 2011, 102(22): 10163–10172 https://doi.org/10.1016/j.biortech.2011.08.030
2	Machado I M, Atsumi S. Cyanobacterial biofuel production. Journal of Biotechnology, 2012, 162(1): 50–56 https://doi.org/10.1016/j.jbiotec.2012.03.005
3	Nozzi N E, Oliver J W, Atsumi S. Cyanobacteria as a platform for biofuel production. Frontiers in Bioengineering and Biotechnology, 2013, 1: 1–6 https://doi.org/10.3389/fbioe.2013.00007
4	Deng M D, Coleman J R. Ethanol synthesis by genetic engineering in cyanobacteria. Applied and Environmental Microbiology, 1999, 65(2): 523–528
5	Dexter J, Fu P. Metabolic engineering of cyanobacteria for ethanol production. Energy & Environmental Science, 2009, 2(8): 857–864 https://doi.org/10.1039/b811937f
6	Gao Z, Zhao H, Li Z, Tan X, Lu X. Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria. Energy & Environmental Science, 2012, 5(12): 9857–9865 https://doi.org/10.1039/C2EE22675H
7	Choi Y N, Park J M. Enhancing biomass and ethanol production by increasing NADPH production in Synechocystis sp. PCC 6803. Bioresource Technology, 2016, 213: 54–57 https://doi.org/10.1016/j.biortech.2016.02.056
8	Kusakabe T, Tatsuke T, Tsuruno K, Hirokawa Y, Atsumi S, Liao J C, Hanai T. Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light. Metabolic Engineering, 2013, 20: 101–108 https://doi.org/10.1016/j.ymben.2013.09.007
9	Lan E I, Liao J C. Metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide. Metabolic Engineering, 2011, 13(4): 353–363 https://doi.org/10.1016/j.ymben.2011.04.004
10	Lan E I, Liao J C. ATP drives direct photosynthetic production of 1-butanol in cyanobacteria. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(16): 6018–6023 https://doi.org/10.1073/pnas.1200074109
11	Atsumi S, Higashide W, Liao J C. Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde. Nature Biotechnology, 2009, 27(12): 1177–1180 https://doi.org/10.1038/nbt.1586
12	Angermayr S A, Paszota M, Hellingwerf K J. Engineering a cyanobacterial cell factory for production of lactic acid. Applied and Environmental Microbiology, 2012, 78(19): 7098–7106 https://doi.org/10.1128/AEM.01587-12
13	Varman A M, Yu Y, You L, Tang Y J. Photoautotrophic production of D-lactic acid in an engineered cyanobacterium. Microbial Cell Factories, 2013, 12(1): 1–8 https://doi.org/10.1186/1475-2859-12-117
14	Zhou J, Zhang H, Meng H, Zhang Y, Li Y. Production of optically pure D-lactate from CO2 by blocking the PHB and acetate pathways and expressing D-lactate dehydrogenase in cyanobacterium Synechocystis sp. PCC 6803. Process Biochemistry, 2014, 49(12): 2071–2077 https://doi.org/10.1016/j.procbio.2014.09.007
15	Angermayr S A, Van der Woude A D, Correddu D, Vreugdenhil A, Verrone V, Hellingwerf K J. Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803. Biotechnology for Biofuels, 2014, 7(1): 1–15 https://doi.org/10.1186/1754-6834-7-99
16	Li C, Tao F, Ni J, Wang Y, Yao F, Xu P. Enhancing the light-driven production of D-lactate by engineering cyanobacterium using a combinational strategy. Scientific Reports, 2015, 5: 1–11
17	Miyake M, Takase K, Narato M, Khatipov E, Schnackenberg J, Shirai M, Kurane R, Asada Y. Polyhydroxybutyrate production from carbon dioxide by cyanobacteria. Applied Biochemistry and Biotechnology, 2000, 84-86(1-9): 991–1002 https://doi.org/10.1385/ABAB:84-86:1-9:991
18	Tyo K E, Jin Y S, Espinoza F A, Stephanopoulos G. Identification of gene disruptions for increased poly-3-hydroxybutyrate accumulation in Synechocystis PCC 6803. Biotechnology Progress, 2009, 25(5): 1236–1243 https://doi.org/10.1002/btpr.228
19	Zhou J, Zhu T, Cai Z, Li Y. From cyanochemicals to cyanofactories: A review and perspective. Microbial Cell Factories, 2016, 15(1): 1–9 https://doi.org/10.1186/s12934-015-0405-3
20	Wang Y, San K Y, Bennett G N. Cofactor engineering for advancing chemical biotechnology. Current Opinion in Biotechnology, 2013, 24(6): 994–999, 99 https://doi.org/10.1016/j.copbio.2013.03.022
21	Akhtar M K, Jones P R. Cofactor Engineering for enhancing the flux of metabolic pathways. Frontiers in Bioengineering and Biotechnology, 2014, 2: 1–6 https://doi.org/10.3389/fbioe.2014.00030
22	Tamoi M, Miyazaki T, Fukamizo T, Shigeoka S. The calvin cycle in cyanobacteria is regulated by CP12 via the NAD(H)/NADP(H) ratio under light/dark conditions. Plant Journal, 2005, 42(4): 504–513 https://doi.org/10.1111/j.1365-313X.2005.02391.x
23	Cooley J W, Vermaas W F. Succinate dehydrogenase and other respiratory pathways in thylakoid membranes of Synechocystis sp. strain PCC 6803: Capacity comparisons and physiological function. Journal of Bacteriology, 2001, 183(14): 4251–4258 https://doi.org/10.1128/JB.183.14.4251-4258.2001
24	Dempo Y, Ohta E, Nakayama Y, Bamba T, Fukusaki E. Molar-based targeted metabolic profiling of cyanobacterial strains with potential for biological production. Metabolites, 2014, 4(2): 499–516 https://doi.org/10.3390/metabo4020499
25	Hirokawa Y, Maki Y, Tatsuke T, Hanai T. Cyanobacterial production of 1,3-propanediol directly from carbon dioxide using a synthetic metabolic pathway. Metabolic Engineering, 2016, 34: 97–103 https://doi.org/10.1016/j.ymben.2015.12.008
26	Li H, Liao J C. Engineering a cyanobacterium as the catalyst for the photosynthetic conversion of CO2 to 1,2-propanediol. Microbial Cell Factories, 2013, 12(1): 1–9 https://doi.org/10.1186/1475-2859-12-4
27	Oliver J W, Machado I M, Yoneda H, Atsumi S. Cyanobacterial conversion of carbon dioxide to 2,3-butanediol. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(4): 1249–1254 https://doi.org/10.1073/pnas.1213024110
28	Savakis P E, Angermayr S A, Hellingwerf K J. Synthesis of 2,3-butanediol by Synechocystis sp. PCC 6803 via heterologous expression of a catabolic pathway from lactic acid-and enterobacteria. Metabolic Engineering, 2013, 20: 121–130 https://doi.org/10.1016/j.ymben.2013.09.008
29	Niederholtmeyer H, Wolfstadter B T, Savage D F, Silver P A, Way J C. Engineering cyanobacteria to synthesize and export hydrophilic products. Applied and Environmental Microbiology, 2010, 76(11): 3462–3466 https://doi.org/10.1128/AEM.00202-10
30	McNeely K, Xu Y, Bennette N, Bryant D A, Dismukes G C. Redirecting reductant flux into hydrogen production via metabolic engineering of fermentative carbon metabolism in a cyanobacterium. Applied and Environmental Microbiology, 2010, 76(15): 5032–5038 https://doi.org/10.1128/AEM.00862-10
31	Kumaraswamy G K, Guerra T, Qian X, Zhang S, Bryant D A, Dismukes G C. Reprogramming the glycolytic pathway for increased hydrogen production in cyanobacteria: Metabolic engineering of NAD+-dependent GAPDH. Energy & Environmental Science, 2013, 6(12): 3722–3731 https://doi.org/10.1039/c3ee42206b
32	Jarboe L R, Yqh D. A broad-substrate range aldehyde reductase with various applications in production of biorenewable fuels and chemicals. Applied Microbiology and Biotechnology, 2011, 89(2): 249–257 https://doi.org/10.1007/s00253-010-2912-9
33	Wee Y J, Kim J N, Ryu H W. Biotechnological production of lactic acid and its recent applications. Food Technology and Biotechnology, 2006, 44(2): 163–172
34	Joseph A, Aikawa S, Sasaki K, Tsuge Y, Matsuda F, Tanaka T, Kondo A. Utilization of lactic acid bacterial genes in Synechocystis sp. PCC 6803 in the production of lactic acid. Bioscience, Biotechnology, and Biochemistry, 2013, 77(5): 966–970 https://doi.org/10.1271/bbb.120921
35	Polizzi K M, Chaparro-Riggers J F, Vazquez-Figueroa E, Bommarius A S. Structure-guided consensus approach to create a more thermostable penicillin G acylase. Biotechnology Journal, 2006, 1(5): 531–536 https://doi.org/10.1002/biot.200600029
36	Terao Y, Miyamoto K, Ohta H. Introduction of single mutation changes arylmalonate decarboxylase to racemase. Chemical Communications, 2006, 34(34): 3600–3602 https://doi.org/10.1039/b607211a
37	Vázquez-Figueroa E, Chaparro-Riggers J, Bommarius A S. Development of a thermostable glucose dehydrogenase by a structure-guided consensus concept. ChemBioChem, 2007, 8(18): 2295–2301 https://doi.org/10.1002/cbic.200700500
38	Jochens H, Bornscheuer U T. Natural diversity to guide focused directed evolution. ChemBioChem, 2010, 11(13): 1861–1866 https://doi.org/10.1002/cbic.201000284
39	Ema T, Nakano Y, Yoshida D, Kamata S, Sakai T. Redesign of enzyme for improving catalytic activity and enantioselectivity toward poor substrates: Manipulation of the transition state. Organic & Biomolecular Chemistry, 2012, 10(31): 6299–6308 https://doi.org/10.1039/c2ob25614b
40	Holmberg N, Ryde U, Bulow L. Redesign of the coenzyme specificity in L-lactate dehydrogenase from bacillus stearothermophilus using site-directed mutagenesis and media engineering. Protein Engineering, Design & Selection, 1999, 12(10): 851–856 https://doi.org/10.1093/protein/12.10.851
41	Ma C, Zhang L, Dai J, Xiu Z. Relaxing the coenzyme specificity of 1,3-propanediol oxidoreductase from Klebsiella pneumoniae by rational design. Journal of Biotechnology, 2010, 146(4): 173–178 https://doi.org/10.1016/j.jbiotec.2010.02.005
42	Richter N, Zienert A, Hummel W. A single-point mutation enables lactate dehydrogenase from Bacillus subtilis to utilize NAD+ and NADP+ as cofactor. Engineering in Life Sciences, 2011, 11(1): 26–36 https://doi.org/10.1002/elsc.201000151
43	Meng H, Liu P, Sun H, Cai Z, Zhou J, Lin J, Li Y. Engineering a D-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors. Scientific Reports, 2016, 6: 1–8
44	Steiner K, Schwab H. Recent advances in rational approaches for enzyme engineering. Computational and Structural Biotechnology Journal, 2012, 2(3): 1–12 https://doi.org/10.5936/csbj.201209010
45	Li Y, Cirino P C. Recent advances in engineering proteins for biocatalysis. Biotechnology and Bioengineering, 2014, 111(7): 1273–1287 https://doi.org/10.1002/bit.25240

Viewed

Full text

Abstract

Cited

Shared

Discussed