1. Department of Bionanotechnology and Gachon Medical Research Institute, Gachon University and Gachon Medical Research Institute, Seongnam, Korea 2. Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology and Division of Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, Korea
The potential applications of nanomaterials used in nanomedicine as ingredients in drug delivery systems and in other products continue to expand. When nanomaterials are introduced into physiological environments and driven by energetics, they readily associate proteins forming a protein corona (PC) on their surface. This PC could result in an alteration of the nanomaterial’s surface characteristics, affecting their interaction with cells due to conformational changes in adsorbed protein molecules. However, our current understanding of nanobiological interactions is still very limited. Utilizing a liquid chromatography–mass spectroscopy/mass spectroscopy technology and a Cytoscape plugin (ClueGO) approach, we examined the composition of the PC for a set of zinc oxide nanoparticles (ZnONP) from cell culture media typically and further analyzed the biological interaction of identified proteins, respectively. In total, 36 and 33 common proteins were investigated as being bound to ZnONP at 5 min and 60 min, respectively. These proteins were further analyzed with ClueGO, a Cytoscape plugin, which provided gene ontology and the biological interaction processes of identified proteins. Proteins bound to the surface of nanoparticles that may modify the structure, therefore the function of the adsorbed protein could be consequently affect the complicated biological processes.
Corresponding Author(s):
Sang-Wook Son,Seong-Soo A. An
引用本文:
. [J]. Frontiers of Chemical Science and Engineering, 2019, 13(1): 90-97.
Vo-Van Giau, Yoon-Hee Park, Kyu-Hwan Shim, Sang-Wook Son, Seong-Soo A. An. Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture media. Front. Chem. Sci. Eng., 2019, 13(1): 90-97.
Inter-α-trypsin inhibitor heavy chain H1 precursor
Inter-α-trypsin inhibitor heavy chain H2 precursor
Kininogen-2 isoform I precursor
Plasma serine protease inhibitor precursor
Prothrombin precursor
Serum albumin precursor
Tetranectin precursor
Vitronectin precursor
Tab.1
Fig.1
5 min
60 min
Centromere protein F
Coagulation factor IX
Complement factor I precursor
Coagulation factor V precursor
Elongation factor 1-α 2
Myosin-viia
Factor XIIa inhibitor precursor
Serotransferrin precursor
Fibrinogen α chain precursor
Pigment epithelium-derived factor precursor
Talin-1
Thrombospondin-1 precursor
Tab.2
Fig.2
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