Crown ether-thiourea conjugates as ion transporters
Zhixing Zhao1, Bailing Tang1, Xiaosheng Yan1(), Xin Wu2(), Zhao Li1, Philip A. Gale2,3(), Yun-Bao Jiang1()
1. Department of Chemistry, College of Chemistry and Chemical Engineering, The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, iChEM, Xiamen University, Xiamen 361005, China 2. School of Chemistry (F11), The University of Sydney, Sydney, NSW 2006, Australia 3. The University of Sydney Nano Institute (Sydney Nano), The University of Sydney, Sydney, NSW 2006, Australia
Na+, Cl‒ and K+ are the most abundant electrolytes present in biological fluids that are essential to the regulation of pH homeostasis, membrane potential and cell volume in living organisms. Herein, we report synthetic crown ether-thiourea conjugates as a cation/anion symporter, which can transport both Na+ and Cl− across lipid bilayers with relatively high transport activity. Surprisingly, the ion transport activities were diminished when high concentrations of K+ ions were present outside the vesicles. This unusual behavior resulted from the strong affinity of the transporters for K+ ions, which led to predominant partitioning of the transporters as the K+ complexes in the aqueous phase preventing the transporter incorporation into the membrane. Synthetic membrane transporters with Na+, Cl‒ and K+ transport capabilities may have potential biological and medicinal applications.
X Wu, E N W Howe, P A Gale. Supramolecular transmembrane anion transport: new assays and insights. Accounts of Chemical Research, 2018, 51(8): 1870–1879 https://doi.org/10.1021/acs.accounts.8b00264
2
T M Fyles. How do amphiphiles form ion-conducting channels in membranes. Lessons from linear oligoesters. Accounts of Chemical Research, 2013, 46(12): 2847–2855 https://doi.org/10.1021/ar4000295
3
A P Davis, D N Sheppard, B D Smith. Development of synthetic membrane transporters for anions. Chemical Society Reviews, 2007, 36(2): 348–357 https://doi.org/10.1039/B512651G
M Konrad, M Vollmer, H H Lemmink, L P W J Van den Heuvel, N Jeck, R Vargas-Poussou, A Lakings, R Ruf, G Deschenes, C Antignac, et al.. Mutations in the chloride channel gene CLCNKB as a cause of classic Bartter syndrome. Journal of the American Society of Nephrology, 2000, 11(8): 1449–1459
6
R Dutzler, E B Campbell, M Cadene, B T Chait, R MacKinnon. X-ray structure of a ClC chloride channel at 3.0 Å reveals the molecular basis of anion selectivity. Nature, 2002, 415(6869): 287–294 https://doi.org/10.1038/415287a
7
H Valkenier, O Akrawi, P Jurček, K Sleziaková, T Lízal, K Bartik, V Šindelář. Fluorinated bambusurils as highly effective and selective transmembrane Cl−/HCO3− antiporters. Chem, 2019, 5(2): 429–444 https://doi.org/10.1016/j.chempr.2018.11.008
8
H J Clarke, E N W Howe, X Wu, F Sommer, M Yano, M E Light, S Kubik, P A Gale. Transmembrane fluoride transport: direct measurement and selectivity studies. Journal of the American Chemical Society, 2016, 138(50): 16515–16522 https://doi.org/10.1021/jacs.6b10694
9
A Roy, H Joshi, R Ye, J Shen, F Chen, A Aksimentiev, H Zeng. Polyhydrazide-based organic nanotubes as efficient and selective artificial iodide channels. Angewandte Chemie International Edition, 2020, 59(12): 4806–4813 https://doi.org/10.1002/anie.201916287
10
N Busschaert, L E Karagiannidis, M Wenzel, C J E Haynes, N J Wells, P G Young, D Makuc, J Plavec, K A Jolliffe, P A Gale. Synthetic transporters for sulfate: a new method for the direct detection of lipid bilayer sulfate transport. Chemical Science (Cambridge), 2014, 5(3): 1118–1127 https://doi.org/10.1039/c3sc52006d
11
X Wu, L W Judd, E N W Howe, A M Withecombe, V Soto-Cerrato, H Li, N Busschaert, H Valkenier, R Perez-Tomas, D N Sheppard, et al.. Nonprotonophoric electrogenic Cl– transport mediated by valinomycin-like carriers. Chem, 2016, 1(1): 127–146 https://doi.org/10.1016/j.chempr.2016.04.002
12
J T Davis, P A Gale, R Quesada. Advances in anion transport and supramolecular medicinal chemistry. Chemical Society Reviews, 2020, 49(16): 6056–6086 https://doi.org/10.1039/C9CS00662A
13
C Ren, F Zeng, J Shen, F Chen, A Roy, S Zhou, H Ren, H Zeng. Pore-forming monopeptides as exceptionally active anion channels. Journal of the American Chemical Society, 2018, 140(28): 8817–8826 https://doi.org/10.1021/jacs.8b04657
14
M J Spooner, H Li, I Marques, P M R Costa, X Wu, E N W Howe, N Busschaert, S J Moore, M E Light, D N Sheppard, et al.. Fluorinated synthetic anion carriers: experimental and computational insights into transmembrane chloride transport. Chemical Science (Cambridge), 2019, 10(7): 1976–1985 https://doi.org/10.1039/C8SC05155K
15
G W Gokel, A Mukhopadhyay. Synthetic models of cation-conducting channels. Chemical Society Reviews, 2001, 30(5): 274–286 https://doi.org/10.1039/b008667n
J Payandeh, T Scheuer, N Zheng, W A Catterall. The crystal structure of a voltage-gated sodium channel. Nature, 2011, 475(7356): 353–358 https://doi.org/10.1038/nature10238
T J Jentsch. Neuronal KCNQ potassium channels: physiology and role in disease. Nature Reviews. Neuroscience, 2000, 1(1): 21–30 https://doi.org/10.1038/35036198
21
M C Sanguinetti, M Tristani-Firouzi. hERG potassium channels and cardiac arrhythmia. Nature, 2006, 440(7083): 463–469 https://doi.org/10.1038/nature04710
D B Simon, F E Karet, J M Hamdan, A D Pietro, S A Sanjad, R P Lifton. Bartter’s syndrome, hypokalaemic alkalosis with hypercalciuria, is caused by mutations in the Na-K-2Cl cotransporter NKCC2. Nature Genetics, 1996, 13(2): 183–188 https://doi.org/10.1038/ng0696-183
24
C C Tong, R Quesada, J L Sessler, P A Gale. Meso-Octamethylcalix[4]pyrrole: an old yet new transmembrane ion-pair transporter. Chemical Communications, 2008, (47): 6321–6323 https://doi.org/10.1039/b814988g
25
M G Fisher, P A Gale, J R Hiscock, M B Hursthouse, M E Light, F P Schmidtchen, C C Tong. 1,2,3-Triazole-strapped calix[4]pyrrole: a new membrane transporter for chloride. Chemical Communications, 2009, 21(21): 3017–3019 https://doi.org/10.1039/b904089g
26
A V Koulov, J M Mahoney, B D Smith. Facilitated transport of sodium or potassium chloride across vesicle membranes using a ditopic salt-binding macrobicycle. Organic & Biomolecular Chemistry, 2003, 1(1): 27–29 https://doi.org/10.1039/b208873h
27
J H Lee, J H Lee, Y R Choi, P Kang, M G Choi, K S Jeong. Synthetic K+/Cl‒-selective symporter across a phospholipid membrane. Journal of Organic Chemistry, 2014, 79(14): 6403–6409 https://doi.org/10.1021/jo501145z
28
X H Yu, X J Cai, X Q Hong, K Y Tam, K Zhang, W H Chen. Synthesis and biological evaluation of aza-crown ether-squaramide conjugates as anion/cation symporters. Future Medicinal Chemistry, 2019, 11(10): 1091–1106 https://doi.org/10.4155/fmc-2018-0595
29
Z Sun, M Barboiu, Y M Legrand, E Petit, A Rotaru. Highly selective artificial cholesteryl crown ether K+-channels. Angewandte Chemie International Edition, 2015, 54(48): 14473–14477 https://doi.org/10.1002/anie.201506430
30
A Gilles, M Barboiu. Highly selective artificial K+ channels: an example of selectivity-induced transmembrane potential. Journal of the American Chemical Society, 2016, 138(1): 426–432 https://doi.org/10.1021/jacs.5b11743
31
Y H Li, S Zheng, Y M Legrand, A Gilles, A van der Lee, M Barboiu. Structure-driven selection of adaptive transmembrane Na+ carriers or K+ channels. Angewandte Chemie International Edition, 2018, 57(33): 10520–10524 https://doi.org/10.1002/anie.201802570
32
S Chen, Y Wang, T Nie, C Bao, C Wang, T Xu, Q Lin, D H Qu, X Gong, Y Yang, L Zhu, H Tian. An artificial molecular shuttle operates in lipid bilayers for ion transport. Journal of the American Chemical Society, 2018, 140(51): 17992–17998 https://doi.org/10.1021/jacs.8b09580
33
F Y Wu, Z Li, L Guo, X Wang, M H Lin, Y F Zhao, Y B Jiang. A unique NH-spacer for N-benzamidothiourea based anion sensors. Substituent effect on anion sensing of the ICT dual fluorescent N-(p-dimethylaminobenzamido)-N′-arylthioureas. Organic & Biomolecular Chemistry, 2006, 4(4): 624–630 https://doi.org/10.1039/b513969d
34
A F Li, J H Wang, F Wang, Y B Jiang. Anion complexation and sensing using modified urea and thiourea-based receptors. Chemical Society Reviews, 2010, 39(10): 3729–3745 https://doi.org/10.1039/b926160p
35
M Villa, G Bergamini, P Ceroni, M Baroncini. Photocontrolled self-assembly of azobenzene nanocontainers in water: light-triggered uptake and release of lipophilic molecules. Chemical Communications, 2019, 55(79): 11860–11863 https://doi.org/10.1039/C9CC05925C
36
Z Du, B Ren, X Chang, R Dong, J Peng, Z Tong. Aggregation and rheology of an azobenzene-functionalized hydrophobically modified ethoxylated urethane in aqueous solution. Macromolecules, 2016, 49(13): 4978–4988 https://doi.org/10.1021/acs.macromol.6b00633
37
F Otis, C Racine-Berthiaume, N Voyer. How far can a sodium ion travel within a lipid bilayer? Journal of the American Chemical Society, 2011, 133(17): 6481–6483 https://doi.org/10.1021/ja110336s
38
Y Yang, X Wu, N Busschaert, H Furuta, P A Gale. Dissecting the chloride-nitrate anion transport assay. Chemical Communications, 2017, 53(66): 9230–9233 https://doi.org/10.1039/C7CC04912A
39
A Vargas Jentzsch, D Emery, J Mareda, P Metrangolo, G Resnati, S Matile. Ditopic ion transport systems: anion-π interactions and halogen bonds at work. Angewandte Chemie International Edition, 2011, 50(49): 11675–11678 https://doi.org/10.1002/anie.201104966
40
N Busschaert, M Wenzel, M E Light, P Iglesias-Hernandez, R Perez-Tomas, P A Gale. Structure-activity relationships in tripodal transmembrane anion transporters: the effect of fluorination. Journal of the American Chemical Society, 2011, 133(35): 14136–14148 https://doi.org/10.1021/ja205884y
41
H Valkenier, C J E Haynes, J Herniman, P A Gale, A P Davis. Lipophilic balance—a new design principle for transmembrane anion carriers. Chemical Science (Cambridge), 2014, 5(3): 1128–1134 https://doi.org/10.1039/c3sc52962b
42
C Ren, J Shen, H Zeng. Combinatorial evolution of fast-conducting highly selective K+-channels via modularly tunable directional assembly of crown ethers. Journal of the American Chemical Society, 2017, 139(36): 12338–12341 https://doi.org/10.1021/jacs.7b04335
43
C Ren, F Chen, R Ye, Y S Ong, H Lu, S S Lee, J Y Ying, H Zeng. Molecular swings as highly active ion transporters. Angewandte Chemie International Edition, 2019, 58(24): 8034–8038 https://doi.org/10.1002/anie.201901833
44
R Ye, C Ren, J Shen, N Li, F Chen, A Roy, H Zeng. Molecular ion fishers as highly active and exceptionally selective K+ transporters. Journal of the American Chemical Society, 2019, 141(25): 9788–9792 https://doi.org/10.1021/jacs.9b04096
45
T Liu, C Bao, H Wang, Y Lin, H Jia, L Zhu. Light-controlled ion channels formed by amphiphilic small molecules regulate ion conduction via cis-trans photoisomerization. Chemical Communications, 2013, 49(87): 10311–10313 https://doi.org/10.1039/c3cc45618h
46
Z Sun, A Gilles, I Kocsis, Y M Legrand, E Petit, M Barboiu. Squalyl crown ether self-assembled conjugates: an example of highly selective artificial K+ channels. Chemistry (Weinheim an der Bergstrasse, Germany), 2016, 22(6): 2158–2164 https://doi.org/10.1002/chem.201503979
47
S Schneider, E D Licsandru, I Kocsis, A Gilles, F Dumitru, E Moulin, J Tan, J M Lehn, N Giuseppone, M Barboiu. Columnar self-assemblies of triarylamines as scaffolds for artificial biomimetic channels for ion and for water transport. Journal of the American Chemical Society, 2017, 139(10): 3721–3727 https://doi.org/10.1021/jacs.6b12094
48
X Wu, J R Small, A Cataldo, A M Withecombe, P Turner, P A Gale. Voltage-switchable HCl transport enabled by lipid headgroup-transporter interactions. Angewandte Chemie International Edition, 2019, 58(42): 15142–15147 https://doi.org/10.1002/anie.201907466
49
X Wu, N Busschaert, N J Wells, Y B Jiang, P A Gale. Dynamic covalent transport of amino acids across lipid bilayers. Journal of the American Chemical Society, 2015, 137(4): 1476–1484 https://doi.org/10.1021/ja510063n
50
S P Zheng, L B Huang, Z Sun, M Barboiu. Self-assembled artificial ion-channels toward natural selection of functions. Angewandte Chemie International Edition, 2021, 60(2): 566–597 https://doi.org/10.1002/anie.201915287