The <italic>rpoS</italic> deficiency suppresses acetate accumulation in glucose-enriched culture of <italic>Escherichia coli</italic> under an aerobic condition

doi:10.1007/s11705-012-1287-0

Front Chem Sci Eng

2012, Vol. 6

Issue (2) : 152-157 https://doi.org/10.1007/s11705-012-1287-0

RESEARCH ARTICLE

The rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichia coli under an aerobic condition

Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA(

)

Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Osaka 560-8531, Japan

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Abstract

The role of Escherichiacoli rpoS on the central carbon metabolism was investigated through analyzing the deficiency of this regulon gene under aerobic and glucose-enriched culture conditions. The experimental results showed that while the wild type cells exhibited an overflow metabolism effect, the rpoS-deleting mutation alleviated this effect with the significant suppression of acetate accumulation under a high glucose condition. This gene deletion also induced the twofold upregulation of gltA and one-tenth downregulation of poxB, respectively. The overflow metabolism effect was confirmed to be recovered by re-introducing rpoS gene into the mutant. These results demonstrated rpoS changed the central carbon metabolism toward acetate overflow through dehydrogenation of pyruvate and reduction of TCA cycle activity.

Keywords Escherichia coli rpoS aerobic and glucose-enriched culture overflow metabolism

Corresponding Author(s): TAYA Masahito,Email:taya@cheng.es.osaka-u.ac.jp

Issue Date: 05 June 2012

Cite this article:

Prayoga SURYADARMA,Yoshihiro OJIMA,Yuto FUKUDA, et al. The rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichia coli under an aerobic condition[J]. Front Chem Sci Eng, 2012, 6(2): 152-157.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-012-1287-0
https://academic.hep.com.cn/fcse/EN/Y2012/V6/I2/152

Tab.1 Primers designed for gene expression analysis

Fig.1 Expressions of selected oxidative stress response genes in wild type strain cultured for 12 h at different oxygenation levels

Fig.2 Time profiles of DCW, glucose, and acetate concentrations in cultures of wild type and deficient mutant strains

Fig.3 Effect of oxygen supply on glucose-based acetate yield in cultures of wild-type and deficient mutant strains for 72 h

Fig.4 (a) Central carbon metabolism and related genes, and (b) expressions of selected genes in wild-type and deficient mutant strains cultured for 12 h at = 4.9 min

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