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Gold nanoparticles/single-stranded DNA-reduced graphene oxide nanocomposites based electrochemical biosensor for highly sensitive detection of cholesterol |
Shuyao Wu1, Chengquan Sui1, Chong Wang1, Yulu Wang1, Dongqing He1, Ying Sun1, Yu Zhang1, Qingbo Meng1, Tianyi Ma2(), Xi-Ming Song1() |
1. Liaoning Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials, College of Chemistry, Liaoning University, Shenyang 110036, China 2. Centre for Translational Atomaterials, Swinburne University of Technology, Hawthorn VIC 3122, Australia |
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Abstract High density and uniform distribution of the gold nanoparticles functionalized single-stranded DNA modified reduced graphene oxide nanocomposites were obtained by non-covalent interaction. The positive gold nanoparticles prepared by phase inversion method exhibited good dimensional homogeneity and dispersibility, which could readily combine with single-stranded DNA modified reduced graphene oxide nanocomposites by electrostatic interactions. The modification of single-stranded DNA endowed the reduced graphene oxide with favorable biocompatibility and provided the preferable surface with negative charge for further assembling of gold nanoparticles to obtain gold nanoparticles/single-stranded DNA modified reduced graphene oxide nanocomposites with better conductivity, larger specific surface area, biocompatibility and electrocatalytic characteristics. The as-prepared nanocomposites were applied as substrates for the construction of cholesterol oxidase modified electrode and well realized the direct electron transfer between the enzyme and electrode. The modified gold nanoparticles could further catalyze the products of cholesterol oxidation catalyzed by cholesterol oxidase, which was beneficial to the enzyme-catalyzed reaction. The as-fabricated bioelectrode exhibited excellent electrocatalytic performance for the cholesterol with a linear range of 7.5−280.5 μmol·L−1, a low detection limit of 2.1 μmol·L−1, good stability and reproducibility. Moreover, the electrochemical biosensor showed good selectivity and acceptable accuracy for the detection of cholesterol in human serum samples.
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Keywords
reduced graphene oxide
gold nanoparticles
electrochemical biosensor
cholesterol oxidase
cholesterol
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Corresponding Author(s):
Tianyi Ma,Xi-Ming Song
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Online First Date: 22 October 2021
Issue Date: 09 November 2021
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