Dendrimer-induced synthesis of porous organosilica capsules for enzyme encapsulation

doi:10.1007/s11705-024-2400-x

Front. Chem. Sci. Eng.

2024, Vol. 18

Issue (4) : 39 https://doi.org/10.1007/s11705-024-2400-x

Dendrimer-induced synthesis of porous organosilica capsules for enzyme encapsulation

Ziyi Chu¹, Boyu Zhang¹, Zhenhua Wu¹, Jiaxu Zhang¹, Yiran Cheng¹, Xueying Wang¹, Jiafu Shi^1,^2,³(

), Zhongyi Jiang^2,⁴

¹. School of Environmental Science & Engineering, Tianjin University, Tianjin 300072, China
². Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China
³. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
⁴. Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

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Abstract

Organic matter-induced mineralization is a green and versatile method for synthesizing hybrid nanostructured materials, where the material properties are mainly influenced by the species of natural biomolecules, linear synthetic polymer, or small molecules, limiting their diversity. Herein, we adopted dendrimer poly(amidoamine) (PAMAM) as the inducer to synthesize organosilica-PAMAM network (OSPN) capsules for mannose isomerase (MIase) encapsulation based on a hard-templating method. The structure of OSPN capsules can be precisely regulated by adjusting the molecular weight and concentration of PAMAM, thereby demonstrating a substantial impact on the kinetic behavior of the MIase@OSPN system. The MIase@OSPN system was used for catalytic production of mannose from D-fructose. A mannose yield of 22.24% was obtained, which is higher than that of MIase in organosilica network capsules and similar to that of the free enzyme. The overall catalytic efficiency (k_cat/K_m) of the MIase@OSPN system for the substrate D-fructose was up to 0.556 s⁻¹·mmol⁻¹·L. Meanwhile, the MIase@OSPN system showed excellent stability and recyclability, maintaining more than 50% of the yield even after 12 cycles.

Keywords enzyme immobilization enzyme catalysis organosilica networks capsules sugar biosynthesis

Corresponding Author(s): Jiafu Shi

Just Accepted Date: 28 December 2023 Issue Date: 15 March 2024

Cite this article:

Ziyi Chu,Boyu Zhang,Zhenhua Wu, et al. Dendrimer-induced synthesis of porous organosilica capsules for enzyme encapsulation[J]. Front. Chem. Sci. Eng., 2024, 18(4): 39.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-024-2400-x
https://academic.hep.com.cn/fcse/EN/Y2024/V18/I4/39

Fig.1 Schematic diagram about the synthesis and catalysis processes of MIase@OSPN.

Fig.2 (a, b) TEM images, (c) FT-IR spectra, (d–f) XPS spectra, (g) pore size distribution, and (h) TGA image of OSPN and OSN capsules.

Fig.3 (a–c) TEM images, (d) FT-IR spectra, and (e–g) pore size distribution of OSPN capsules with different molecular weights of PAMAM.

Fig.4 (a–c) TEM images, (d) FT-IR spectra, and (e–g) pore size distribution of OSPN capsules with different concentrations of PAMAM.

Fig.5 (a, d) Yield of MIase@OSN and MIase@OSPN systems; (b, e) k_cat/K_m; (c, f) leakage ratio of MIase@OSPN with different molecular weights and different concentrations of PAMAM; (g) stability of MIase, MIase@OSN and MIase@OSPN under different pH incubation conditions; (h) different temperature incubation conditions; (i) recyclability of MIase@OSN and MIase@OSPN. The horizontal black lines in (a) and (d) represent the yield of MIase@OSN.

Tab.1 Kinetic parameters of OSPN with different molecular weights of PAMAM

Tab.2 Kinetic parameters of OSPN with different concentrations of PAMAM

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