Distributions of phospholipid and glycolipid fatty acids in two strains of different functional <italic>Erythrobacter</italic> sp. isolated from South China Sea

doi:10.1007/s11707-009-0009-5

Front Earth Sci Chin

2009, Vol. 3

Issue (1) : 91-99 https://doi.org/10.1007/s11707-009-0009-5

RESEARCH ARTICLE

Distributions of phospholipid and glycolipid fatty acids in two strains of different functional Erythrobacter sp. isolated from South China Sea

Huan YANG^1,3(

), Xiangru MA1¹, Qiang LI², Nianzhi JIAO², Shucheng XIE^1,3

1. Key Laboratory of Biogeology and Environmental Geology of Ministry of Education, China University of Geosciences, Wuhan 430074, China; 2. State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen 361005, China; 3. State Key Laboratory of Geological Processes and Mineral Resources, China University of Geosciences, Wuhan 430074, China

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Abstract

The comparison of the fatty acids between aerobic anoxygenic phototrophic bacteria (AAPB) and their phylogenetic relatives has been a fascinating but yet enigmatic topic, enhancing our understanding of physiological variations between these evolutionarily related microorganisms. Two strains of marine bacteria, both phylogenetically falling into Erythrobacter sp., were isolated from the South China Sea, and demonstrated, respectively, to be an aerobic anoxygenic phototrophic bacteria (AAPB) (JL475) which is capable of anoxygenic photosynthesis via BChl a, and an obligate heterotroph (JL316) with a lack of BChl a, on the basis of phylogenetic analysis and pure culture cultivation. Phospholipid fatty acids (PLFA) and glycolipid fatty acids (GLFA) of the two strains were extracted and analyzed by gas chromatography-mass spectrometry. The PLFA in JL475 AAPB are characterized by C_18∶1, C_18∶2_ω_7,13 and C_18∶0, with the C_18∶2_ω_7,13 being a specific compound for AAPB and in particular for Erythrobacter longus and some of its phylogenetically closely related relatives. The JL316 strain is characterized in PLFA by the presence of C_18∶1, C_16∶1 and C_16∶0, and in particular C_17∶1. GLFA do not show any discrimination between the two strains. Four α,ω-dicarboxylic acids, including 1,8-octanedioic acid, 1,9-nonanedioic acid, 1,10-decanedioic acid and 1,11-undecanedioic acid, are present only in JL316 GLFA, presumably derived from metabolic products. C₁₄—C₁₆ 2-hydroxy fatty acids were found in the two strains, probably assuming a similar function of their LPS in outer membranes.

Keywords AAPB PLFA GLFA hydroxy fatty acids Erythrobacter

Corresponding Author(s): YANG Huan,Email:yanghuansailing@hotmail.com

Issue Date: 05 March 2009

Cite this article:

Huan YANG,Xiangru MA1,Qiang LI, et al. Distributions of phospholipid and glycolipid fatty acids in two strains of different functional Erythrobacter sp. isolated from South China Sea[J]. Front Earth Sci Chin, 2009, 3(1): 91-99.

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https://academic.hep.com.cn/fesci/EN/10.1007/s11707-009-0009-5
https://academic.hep.com.cn/fesci/EN/Y2009/V3/I1/91

Tab.1 Sample information of the two strains

Fig.1 Phylogenetic positions of JL475 and JL316 based on 16S rRNA analysis. The scale bar represents 0.5 substitution per 100 nucleotide positions

Tab.2 PLFA and their relative abundance

Fig.2 Comparison of mass chromatographs of GLFA, diethyl esters between two bacteria (m/z 88). a,b,c,d deonte1,8–octanedioic acid, 1,9–nonanedioic acid, 1,10–decanedioic acid and 1,11–undecanedioic acid, respectively

Tab.3 GLFA and their relative abundance

Fig.3 Mass spectra of 1,8–octanedioic acid, diethyl ester and its fragmentation pattern

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