Optimization of acid digestion conditions on the extraction of fatty acids from stalagmites

doi:10.1007/s11707-012-0311-5

Front Earth Sci

2012, Vol. 6

Issue (1) : 109-114 https://doi.org/10.1007/s11707-012-0311-5

SHORT COMMUNICATION

Optimization of acid digestion conditions on the extraction of fatty acids from stalagmites

Canfa WANG^1,2, Hongbin ZHANG², Xianyu HUANG², Junhua HUANG², Shucheng XIE¹(

)

1. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, China; 2. State Key Laboratory of Geological Processes and Mineral Resources, China University of Geosciences, Wuhan 430074, China

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Abstract

Lipids in stalagmites have been shown the potential for the paleoclimate reconstruction. However, the low lipid content leads to the difficulty in gaining high resolution lipid record in stalagmites because large mass of samples are required. Previous studies have validated that the acid digestion can improve the yield of lipids, especially fatty acids (FAs) and 3-hydroxy fatty acids (3-OH-FAs). In order to obtain more content of FAs and 3-OH-FAs with limited stalagmite sample weight, we investigate here how the acid digestion conditions (HCl concentration, heating temperature and time duration) could affect the yields of FAs and 3-OH-FAs. Under different concentration of HCl, from 2.0 to 6.0 mol/L, the FAs keep the same step in content variation with 3-OH-FAs, and the highest yields of both two appeared under the 3 mol/L HCl. The content of 3-OH-FAs increases positively with the heating temperature from 80°C to 150°C, while FAs showed the highest content at 130°C. Both of FAs and 3-OH-FAs firstly increased to a high content and then decreased as the heating time duration varies from 1.0 to 4.0 h, with the highest yields of both two being at 3.0 h. Consequently, we suggest the optimized acid digestion condition is under 3 mol/L HCl, heating at 130°C for 3 h and 5 g of each stalagmite sample are sufficient for the lipid analysis.

Keywords stalagmite lipids acid digestion optimization

Corresponding Author(s): XIE Shucheng,Email:xiecug@163.com

Issue Date: 05 March 2012

Cite this article:

Canfa WANG,Hongbin ZHANG,Xianyu HUANG, et al. Optimization of acid digestion conditions on the extraction of fatty acids from stalagmites[J]. Front Earth Sci, 2012, 6(1): 109-114.

URL:

https://academic.hep.com.cn/fesci/EN/10.1007/s11707-012-0311-5
https://academic.hep.com.cn/fesci/EN/Y2012/V6/I1/109

Tab.1 Acid digestion conditions set for optimizing extraction method

Tab.2 The yields of FAs and 3-OH-FAs by different acid digestion conditions

Fig.1 / 74 mass chromatogram of FAs in a stalagmite sample

Fig.2 Mass spectrum of 3-OH C fatty acid as Me/TMS derivative

Fig.3 / 175 mass chromatogram of 3-OH-FAs in a stalagmite sample

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