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Frontiers of Earth Science

ISSN 2095-0195

ISSN 2095-0209(Online)

CN 11-5982/P

Postal Subscription Code 80-963

2018 Impact Factor: 1.205

Front. Earth Sci.    2015, Vol. 9 Issue (1) : 40-50    https://doi.org/10.1007/s11707-014-0448-5
RESEARCH ARTICLE
Application of geo-microbial prospecting method for finding oil and gas reservoirs
M. A. Rasheed1,*(),Syed Zaheer Hasan1,P. L. Srinivasa Rao1,Annapurna Boruah1,V. Sudarshan2,B. Kumar1,T. Harinarayana1
1. Petroleum Research Wing, Gujarat Energy Research and Management Institute (GERMI), Gandhinagar, Gujarat 382007, India
2. Department of Applied Geochemistry, Osmaina University, Hyderabad 500007, India
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Abstract

Microbial prospecting of hydrocarbons is based on the detection of anomalous population of hydrocarbon oxidizing bacteria in the surface soils, indicates the presence of subsurface oil and gas accumulation. The technique is based on the seepage of light hydrocarbon gases such as C1–C4 from the oil and gas pools to the shallow surface that provide the suitable conditions for the development of highly specialized bacterial population. These bacteria utilize hydrocarbon gases as their only food source and are found enriched in the near surface soils above the hydrocarbon bearing structures. The methodology involves the collection of soil samples from the survey area, packing, preservation and storage of samples in pre-sterilized sample bags under aseptic and cold conditions till analysis and isolation and enumeration of hydrocarbon utilizing bacteria such as methane, ethane, propane, and butane oxidizers. The contour maps for the population density of hydrocarbon oxidizing bacteria are drawn and the data can be integrated with geological, geochemical, geophysical methods to evaluate the hydrocarbon prospect of an area and to prioritize the drilling locations thereby reducing the drilling risks and achieve higher success in petroleum exploration. Microbial Prospecting for Oil and Gas (MPOG) method success rate has been reported to be 90%. The paper presents details of microbial prospecting for oil and gas studies, excellent methodology, future development trends, scope, results of study area, case studies and advantages.

Keywords hydrocarbon utilizing bacteria      microbial prospecting      micro-seepage      petroleum exploration     
Corresponding Author(s): M. A. Rasheed   
Online First Date: 15 July 2014    Issue Date: 04 February 2015
 Cite this article:   
M. A. Rasheed,Syed Zaheer Hasan,P. L. Srinivasa Rao, et al. Application of geo-microbial prospecting method for finding oil and gas reservoirs[J]. Front. Earth Sci., 2015, 9(1): 40-50.
 URL:  
https://academic.hep.com.cn/fesci/EN/10.1007/s11707-014-0448-5
https://academic.hep.com.cn/fesci/EN/Y2015/V9/I1/40
Fig.1  Hydrocarbon micro-seepage model and hydrocarbon-induced effects on soils and sediments (after Schumacher and Abrams, 1996).
Parameters Propane oxidizing bacteria(POB)/(cfu·gm–1)
Minimum count 0.6×102
Maximum count 6.86×105
Arithmetic mean 3.93×104
Standard deviation (σ) 6.37×104
Tab.1  Statistical analysis of propane oxidizing bacteria
Parameters Propane oxidizing bacteria(POB)
Total No. of samples 150
Positive samples 83.6%
Nil samples 16.4%
Positive samples>Mean 26 .7%
Positive samples>S.D 12.5%
Tab.2  Percentage analysis of propane oxidizing bacteria.
Fig.2  Pie diagram showing the percentage of propane oxidizing bacteria (POB).
Fig.3  Propane oxidizing bacterial anomaly map of study area, Gujarat.
Fig.4  Integrated map of Mesozoic sediment thickness with hydrocarbon utilizing bacteria
Fig.5  Integrated map of sediment resistivity with hydrocarbon utilizing bacteria.
Fig.6  Integrated map of lineament with hydrocarbon utilizing bacteria.
Fig.7  Integrated map of gravity with hydrocarbon utilizing bacteria.
Fig.8  An East-West Profile across the Saurashtra Peninsula showing the occurrence of a relatively thick sedimentary succession towards the western Part of the basin (after Harinarayana, 2008).
Fig.9  Generalized lithostratigraphy of the Lodhika-1 well (after Singh et al., 1997).
Fig.10  Map of Kietz Oil field detected by microbial prospecting techniques (After Wagner et al., 2002).
Fig.11  Results of drilled wells in microbial anomalies using microbial surveys for oil technique (MOST) (modified after Schumacher, 2003).
1 Dionisi H M, Harms G, Layton A C, Gregory I R, Parker J, Hawkins S A, Robinson K G, Sayler G S (2003). Power analysis for real-time PCR quantification of genes in activated sludge and analysis of the variability introduced by DNA extraction. Appl Environ Microbiol, 69(11): 6597–6604
https://doi.org/10.1128/AEM.69.11.6597-6604.2003
2 Harinarayana T (2008). Applications of magnetotelluric studies in India. Memoir Geological Society of India, 68: 337–356
3 He J Z, Shen J P, Zhang L M, Zhu Y G, Zheng Y M, Xu M G, Di H J (2007). Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices. Environ Microbiol, 9(9): 2364–2374
https://doi.org/10.1111/j.1462-2920.2007.01358.x
4 Hugenholtz P, Goebel B, Pace N R (1998). Impact of cultured independent studies on emerging phylogenetic view of bacteria diversity. J Bacteriol, 180: 4765–4774
5 Kaster K M, Bonaunet K, Berland H, Kjeilen-Eilertsen G, Brakstad O G (2009). Characterisation of cultured-independent and -dependent microbial communities in a high-temperature offshore chalk petroleum reservoir. Antonie van Leeuwenhoek, 96(4): 423–439
https://doi.org/10.1007/s10482-009-9356-1
6 Lakshmi M, Rasheed M A, Madhavi T, Patil D J, Dayal A M (2012). Characterization of light gaseous hydrocarbons using geochemical and microbiological techniques in the near surface soils of Krishna-Godavari Basin, Andhra Pradesh, India. J Environ Biol, 33: 67–79
7 Lysnes K, Bodtker G, Torsvik T, Bjornestad E ?, Sunde E (2009). Microbial response to reinjection of produced water in an oil reservoir. Appl Environ Microbiol, 83(6): 1143–1157
https://doi.org/10.1007/s00253-009-2015-7
8 Mani D, Kumar T S, Rasheed M A, Patil D J, Dayal A M, Rao T G, Balaram V (2011). Soil Iodine determination in Deccan Syneclise, India, implications to near surface geochemical exploration. Nat Resour Res, 20: 75–88
https://doi.org/10.1007/s11053-010-9134-9
9 Miqueletto P B, Andreote F D, Dias A C, Ferreira J C, Dos Santos Neto E V, de Oliveira V M (2011). Cultivation-independent methods applied to the microbial prospection of oil and gas in soil from a sedimentary basin in Brazil. AMB Express, 1(1): 35
https://doi.org/10.1186/2191-0855-1-35
10 Pareja L (1994). Combined microbial, seismic surveys predict oil and gas occurrences in Bolivia. Oil Gas J, 24: 68–70
11 Rasheed M A, Kalpana M S, Veena Prasanna M, Lakshmi M, Madhavi T, Tiwari D M, Patil D J, Dayal A M, Raju S V (2012a). Geo-microbial and light gaseous hydrocarbon anomalies in the near surface soils of Deccan Syneclise Basin, India: implications to hydrocarbon resource potential. J Petrol Sci Eng, 84–85: 33–41
https://doi.org/10.1016/j.petrol.2012.01.010
12 Rasheed M A, Lakshmi M, Rao P L S, Kalpana M S, Dayal A M, Patil D J (2013a). Geochemical evidences of trace metal anomalies for finding hydrocarbon microseepage in the petroliferous regions of Tatipaka and Pasarlapudi areas of Krishna Godavari Basin, India. Petroleum Science, 10(1): 19–29
https://doi.org/10.1007/s12182-013-0245-x
13 Rasheed M A, Lakshmi M, Rao P L S, Patil D J, Dayal A M, Sudarshan V (2012b). Relevance of Pentane and hexane utilizing bacterial indicators for finding hydrocarbon micro seepage: a study from Jamnagar Sub-basin, Saurashtra, Gujarat, India. Nat Resour Res, 21(4): 427–441
https://doi.org/10.1007/s11053-012-9189-x
14 Rasheed M A, Lakshmi M, Srinu D, Dayal A M (2011). Bacteria as indicators for finding oil and gas reservoirs: a case study of Bikaner-Nagaur Basin, Rajasthan, India. Petroleum Science, 8(3): 264–268
https://doi.org/10.1007/s12182-011-0143-z
15 Rasheed M A, Patil D J, Dayal A M (2013b). Hydrocarbons. In: Microbial Techniques for Hydrocarbon Exploration, ISBN 979-953-307-936-2: INTECH Publishers, 195–218
16 Rasheed M A, Veena Prasanna M, Lakshmi M, Madhavi T, Kalpana M S, Patil D J, Dayal A M (2012c). Geo-microbial prospecting studies of surface sediments from petroliferous region of the Mehsana block, North Cambay Basin, India. J Geol Soc India, 80(2): 267–275
https://doi.org/10.1007/s12594-012-0137-5
17 Rasheed M A, Veena Prasanna M, Satish Kumar T, Patil D J, Dayal A M (2008). Geo-microbial prospecting method for hydrocarbon exploration in Vengannapalli village, Cuddapah Basin, India. Curr Sci, 95: 361–366
18 Schumacher D (2003). Geochemical exploration for oil and gas-strategies for?success: an exploration short course sponsored by National Geophysical?Research Institute, Hyderabad, India, 1–175
19 Schumacher D, Abrams M A (1996). Hydrocarbon migration and its near surface expression. AAPG Mem, 66: 446
20 Singh D, Alat C A, Singh R N, Gupta V P (1997). Source rock characterization and hydrocarbon generating potential of Mesozoic sediments in Lodhika area, Saurashtra Basin, Gujarat. In: Proeedings second Int. Pet. Conf. and Exbn. Petrotech, New Delhi, 205–220
21 Skovhus T L, Ramsing N B, Holmstr?m C, Kjelleberg S, Dahll?f I (2004). Real-time quantitative PCR for assessment of abundance of Pseudoalteromonas species in marine samples. Appl Environ Microbiol, 70(4): 2373–2382
https://doi.org/10.1128/AEM.70.4.2373-2382.2004
22 Tucker J, Hitzman D (1994). Detailed microbial surveys help improve reservoir characterization. Oil Gas J, 6: 65–69
23 Veena Prasanna M, Rasheed M A, Patil D J, Dayal A M (2013). Geo-microbiological studies in conjunction with different geo-scientific studies for the evaluation of hydrocarbon prospects in Proterozoic Vindhyan Basin, India. Journal of Petroleum Science and Engineering, Available online, <day>29</day><month>May</month>2013 http://dx.doi.org/10.1016/j.petrol.2013.04.010
24 Wagner M, Wagner M, Piske J, Smit R (2002). Case histories of microbial prospection for oil and gas. AAPG Studies in Geology 48 and SEG Geophysical References Series, 11: 453–479
25 Zhang F, She Y, Zheng Y, Zhou Z, Kong S, Hou D (2010). Molecular biologic techniques applied to the microbial prospecting of oil and gas in the Ban 876 gas and oil field in China. Appl Microbiol Biotechnol, 86(4): 1183–1194
https://doi.org/10.1007/s00253-009-2426-5
[1] MA Liqiao, DONG Yong, TU Xiaolong, SUN Fengxia, XIA Jiufeng, YANG Lanying. Petroleum prospects of marine sequences in South China[J]. Front. Earth Sci., 2008, 2(1): 105-113.
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