Complementarity of lacustrine pollen and sedimentary DNA in representing vegetation on the central-eastern Tibetan Plateau

doi:10.1007/s11707-022-1075-1

2023, Vol. 17

Issue (4) : 1037-1048 https://doi.org/10.1007/s11707-022-1075-1

Complementarity of lacustrine pollen and sedimentary DNA in representing vegetation on the central-eastern Tibetan Plateau

Fang TIAN¹(

), Meijiao CHEN¹, Weihan JIA^2,³, Ulrike HERZSCHUH^2,^3,⁴, Xianyong CAO⁵

¹. College of Resource Environment and Tourism, Capital Normal University, Beijing 100048, China
². Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Polar Terrestrial Environmental Systems Research Group, Potsdam 14473, Germany
³. University of Potsdam, Institute of Environmental Science and Geography, Potsdam 14476, Germany
⁴. University of Potsdam, Institute of Biochemistry and Biology, Potsdam 14476, Germany
⁵. Group of Alpine Paleoecology and Human Adaptation (ALPHA), State Key Laboratory of Tibetan Plateau Earth System, Environment and Resources (TPESER), Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China

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Abstract

Plant environmental DNA extracted from lacustrine sediments (sedimentary DNA, sedDNA) has been increasingly used to investigate past vegetation changes and human impacts at a high taxonomic resolution. However, the representation of vegetation communities surrounding the lake is still unclear. In this study, we compared plant sedDNA metabarcoding and pollen assemblages from 27 lake surface-sediment samples collected from alpine meadow on the central-eastern Tibetan Plateau to investigate the representation of sedDNA data. In general, the identified components of sedDNA are consistent with the counted pollen taxa and local plant communities. Relative to pollen identification, sedDNA data have higher taxonomic resolution, thus providing a potential approach for reconstructing past plant diversity. The sedDNA signal is strongly influenced by local plants while rarely affected by exogenous plants. Because of the overrepresentation of local plants and PCR bias, the abundance of sedDNA sequence types is very variable among sites, and should be treated with caution when investigating past vegetation cover and climate based on sedDNA data. Our finding suggests that sedDNA analysis can be a complementary approach for investigating the presence/absence of past plants and history of human land-use with higher taxonomic resolution.

Keywords Tibetan Plateau plant DNA metabarcoding pollen lake sediment plant diversity vegetation history

Corresponding Author(s): Fang TIAN

Just Accepted Date: 22 September 2023 Online First Date: 10 January 2024 Issue Date: 06 February 2024

Cite this article:

Fang TIAN,Meijiao CHEN,Weihan JIA, et al. Complementarity of lacustrine pollen and sedimentary DNA in representing vegetation on the central-eastern Tibetan Plateau[J]. Front. Earth Sci., 2023, 17(4): 1037-1048.

URL:

https://academic.hep.com.cn/fesci/EN/10.1007/s11707-022-1075-1
https://academic.hep.com.cn/fesci/EN/Y2023/V17/I4/1037

Fig.1 Location of the 27 sampled lakes (red dots) on the central-eastern Tibetan Plateau. Vegetation map is obtained from the “Atlas of Tibetan Plateau” (Institute of Geographic Sciences and Natural Resources Research, 1990).

Tab.1 Location, elevation, and vegetation type of the 27 lake surface samples

Fig.2 Pollen percentages diagram for the 27 lake samples from the central-eastern Tibetan Plateau ordered by mean annual precipitation P_ann. Pollen taxa presented here are included in the ordination analyses.

Fig.3 Plant sedDNA percentages of samples from 27 lakes on the central-eastern Tibetan Plateau. Plant sedDNA sequence types shown here are included in the ordination analyses.

Tab.2 Exclusive plant types identified by pollen and sedDNA

Pollen	sedDNA
Picea	Picea
Salix	Salix
Chenopodiaceae	Amaranthaceae
Ephedra	Ephedra_st1
Ericaceae	Rhododendron lapponicum
Euphorbiaceae	Euphorbia_st2
Fabaceae	Astragalus variabilis, Astragalus_st1, Astragalus_st2, Caragana jubata, Hedysarum, Hedysarum tibeticum, Oxytropis, Oxytropis deflexa, Pisum
Hippophae	Hippophae tibetana_st1, Hippophae tibetana_st2, Hippophae rhamnoides
Rosaceae	Alchemilla, Aruncus dioicus, Colurieae, Comarum palustre, Fragariinae_st2, Maleae, Potentilla anserina, Potentilla_st2, Potentilla_st3, Spiraea
Tamaricaceae	Myricaria, Myricaria germanica
Apiaceae	Apiaceae_st2, Apioideae_st3, Apioideae_st4, Carum carvi, Selineae
Artemisia
Asterceae	Anthemideae_st1, Asteraceae_st7, Asteraceae_st8, Asteroideae_st11, Carduinae, Gnaphalieae
Cyperaceae	Blysmus compressus, Carex atrofusca, Carex maritima, Carex microglochin, Carex parva, Carex supina, Carex_st1, Carex_st2, Kobresia_st1, Kobresia_st2, Trichophorum pumilum
Urticaceae	Urtica_st2
Gentianaceae	Gentiana_st1, Gentiana_st2, Gentianeae, Gentianella arenaria, Halenia elliptica
Lamiaceae	Dracocephalum ruyschiana, Elsholtzia, Elsholtzia densa, Lamiaceae_st3, Salvia, Thymus
Plantaginaceae	Lagotis glauca, Plantago, Veronica, Veronica chamaedrys
Onagraceae	Epilobium
Papaveraceae	Corydalis, Meconopsis
Poaceae	Agrostidinae, Festuca, Hordeum, Stipa breviflora, Poaceae, Poeae_st1, Poeae_st2, Pooideae_st1, Pooideae_st2, Puccinellia, Triticeae
Polygonum	Aconogonon, Bistorta vivipara, Knorringia sibirica, Koenigia islandica, Rheum_st1, Rheum_st2
Primulaceae	Androsace, Androsace chamaejasme, Primula, Primula nutans
Ranunculaceae	Aconitum lycoctonum, Adonis, Anemone_st1, Caltha scaposa, Caltha sinogracilis, Caltha_st4, Caltha_st5, Delphinieae, Delphinium, Oxygraphis glacialis, Ranunculaceae_st2, Ranunculeae_st2, Ranunculus abortivus, Ranunculus arcticus, Ranunculus auricomus, Thalictrum_st2, Trollius
Saxifragaceae	Ribes glandulosum, Saxifraga hirculus, Saxifraga_st2
Orobanchaceae	Castilleja pallida var. hyparctica, Castilleja_st4, Castilleja_st5, Castilleja_st6, Euphrasia, Pedicularis elwesii, Pedicularis kansuensis, Pedicularis longiflora, Pedicularis oederi_st1, Pedicularis oederi_st2, Pedicularis tristis, Pedicularis verticillata_st1, Pedicularis verticillata_st2, Pedicularis verticillata_st3, Pedicularis_st4, Lancea tibetica

Tab.3 Common plant types identified by pollen and sedDNA

Fig.4 Comparison between pollen and sedDNA percentages for six major families in the 27 lake surface-sediment samples (horizontal axis: pollen percentages; vertical axis: sedDNA percentages).

Tab.4 Summary statistics of redundancy analysis (RDA) of 26 pollen taxa and climatic variables

Fig.5 Redundancy analysis of our 27 lake surface-sediment samples with climate variables (black arrows) based on pollen taxa (A) and plant sedDNA sequence at the family level (B) shown by black circles.

Tab.5 Summary statistics of redundancy analysis (RDA) of 22 families of sedDNA sequence and climatic variables

Tab.6 Summary statistics of redundancy analysis (RDA) of 69 sedDNA sequence types and climatic variables

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