1. Key Laboratory of Urban Environment and Health Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; 2. Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK; 3. College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China
Pyrene, a representative polycyclic aromatic hydrocarbon (PAH) compound produced mainly from incomplete combustion of fossil fuels, is hazardous to ecosystem health. However, long-term exposure studies did not detect any significant effects of pyrene on soil microorganism. In this study, short-term microcosm experiments were conducted to identify the immediate effect of pyrene on soil bacterial communities. A freshly-collected pristine red soil was spiked with pyrene at 0, 10, 100, 200, and 500 mg·kg-1 and incubated for one day and seven days. The bacterial communities in the incubated soils were analyzed using 16S rRNA sequencing and terminal restriction fragment length polymorphism (T-RFLP) methods. The results revealed high bacterial diversity in both unspiked and pyrene-spiked soils. Only at the highest pyrene-spiking rate of 500 mg·kg-1, two minor bacteria groups of the identified 14 most abundant bacteria groups were completely suppressed. Short-term exposure to pyrene resulted in dominance of Proteobacteria in soil, followed by Acidobacteria, Firmutes, and Bacteroidetes. Our findings showed that bacterial community structure did respond to the presence of pyrene but recovered rapidly from the perturbation. The intensity of impact and the rate of recovery showed some pyrene dosage-dependent trends. Our results revealed that different levels of pyrene may affect the bacterial community structure by suppressing or selecting certain groups of bacteria. It was also found that the bacterial community was most susceptible to pyrene within one day of the chemical addition.
Corresponding Author(s):
CAI Chao,Email:ccai@iue.ac.cn
引用本文:
. Response of bacterial communities to short-term pyrene exposure in red soil[J]. Frontiers of Environmental Science & Engineering, 2013, 7(4): 559-567.
Jingjing PENG, Hong LI, Jianqiang SU, Qiufang ZHANG, Junpeng RUI, Chao CAI. Response of bacterial communities to short-term pyrene exposure in red soil. Front Envir Sci Eng, 2013, 7(4): 559-567.
relative abundance with 500 mg·kg-1 pyrene /(% of total taxa)
Acidobacteria
33
24
22.4
20.7
Verrucomicrobia
4
3
2.7
2.6
Cyanobacteria
6
1
4.1
0.9
Chloroflexi
1
3
0.7
2.6
Bacteroidetes
11
3
7.5
2.6
Planctomycetes
2
3
1.4
2.6
Gemmatimonadetes
2
2
1.4
1.7
Actinobacteria
5
0
3.4
0.0
Firmutes
11
19
7.5
16.4
α-Proteobacteria
24
19
16.3
16.4
β-Proteobacteria
27
26
18.4
22.4
Δ-Proteobacteria
9
10
6.1
8.6
γ-Proteobacteria
9
0
6.1
0.0
unclassified bacteria
3
3
2.0
2.6
total (all taxa)
147
116
100.0
100.0
Tab.1
Fig.2
Fig.3
Fig.4
pyrene concentration
day1st/day7th
day1st control/ day1st treatments
day7th control/day7th treatments
R
P
R
P
R
P
0 ppm
0.222
0.150
10 ppm
0.074
0.250
0.290
0.150
0.190
0.150
100 ppm
0.185
0.350
0.200
0.001a)
0.850
0.001a)
200 ppm
0.629
0.001a)
0.560
0.001a)
0.700
0.001a)
500 ppm
0.556
0.001a)
0.410
0.001a)
0.480
0.001a)
Tab.2
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