Microbial community structure and dynamics of starch-fed and glucose-fed chemostats during two years of continuous operation

doi:10.1007/s11783-015-0815-9

Frontiers of Environmental Science & Engineering

2016, Vol. 10

Issue (2): 368-380 https://doi.org/10.1007/s11783-015-0815-9

本期目录

Microbial community structure and dynamics of starch-fed and glucose-fed chemostats during two years of continuous operation

Min GOU¹,Jing ZENG¹,Huizhong WANG¹,Yueqin TANG^1,^*(

),Toru SHIGEMATSU²,Shigeru MORIMURA³,Kenji KIDA¹

1. College of Architecture and Environment, Sichuan University, Chengdu 610065, China
2. Department of Food Science, Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences (NUPALS), Niigata 956-8603, Japan
3. Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-0862, Japan

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Abstract：

The microbial community structures of two mesophilic anaerobic chemostats, one fed with glucose, the other with starch as sole carbon sources, were studied at various dilution rates (0.05–0.25 d⁻¹ for glucose and 0.025–0.1 d⁻¹ for starch) during two years continuous operation. In the glucose-fed chemostat, the aceticlastic methanogen Methanosaeta spp. and hydrogenotrophic methanogen Methanoculleus spp. predominated at low dilution rates, whereas Methanosaeta spp. and the hydrogenotrophic Methanobacterium spp. predominated together when dilution rates were greater than 0.1 d⁻¹. Bacteria affiliated with the phyla Bacteroidetes, Spirochaetes, and Actinobacteria predominated at dilution rates of 0.05, 0.1, and 0.15 d⁻¹, respectively, while Firmicutes predominated at higher dilution rates (0.2 and 0.25 d⁻¹). In the starch-fed chemostat, the aceticlastic and hydrogenotrophic methanogens coexisted at all dilution rates. Although bacteria belonging to only two phyla were mainly responsible for starch degradation (Spirochaetes at the dilution rate of 0.08 d⁻¹ and Firmicutes at other dilution rates), different bacterial genera were identified at different dilution rates. With the exception of Archaea in the glucose-fed chemostat, the band patterns revealed by denaturing gradient gel electrophoresis (DGGE) of the microbial communities in the two chemostats displayed marked changes during long-term operation at a constant dilution rate. The bacterial community changed with changes in the dilution rate, and was erratic during long-term operation in both glucose-fed and starch-fed chemostats.

Key words： microbial community glucose degradation starch degradation dilution rate continuous methane fermentation phylogenetic analysis

收稿日期: 2015-06-02 出版日期: 2016-02-01

Corresponding Author(s): Yueqin TANG

引用本文:

. [J]. Frontiers of Environmental Science & Engineering, 2016, 10(2): 368-380.
Min GOU,Jing ZENG,Huizhong WANG,Yueqin TANG,Toru SHIGEMATSU,Shigeru MORIMURA,Kenji KIDA. Microbial community structure and dynamics of starch-fed and glucose-fed chemostats during two years of continuous operation. Front. Environ. Sci. Eng., 2016, 10(2): 368-380.

链接本文:

https://academic.hep.com.cn/fese/CN/10.1007/s11783-015-0815-9
https://academic.hep.com.cn/fese/CN/Y2016/V10/I2/368

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