Comparative study on microbial community in intermittently aerated sequencing batch reactors (SBR) and a traditional SBR treating digested piggery wastewater
1. Zhejiang Provincial Key Laboratory of Water Science and Technology, Department of Environment in Yangtze Delta Region Institute of Tsinghua University, Zhejiang, Jiaxing 314006, China 2. School of Environment, Tsinghua University, Beijing 100084, China 3. Aqua Development Center, Mitsubishi Rayon Co. Ltd., Toyohashi 4408601, Japan
IASBRs achieved a higher level of TN and NH4+-N removals than the SBR.
IASBRs had higher abundance of denitrification–related bacteria than the SBR.
The denitrifiers abundance was correlated with the TN removal rate.
The NH4+–N removal rate might relate to the AOB activity.
A traditional sequencing batch reactor (SBR) and two intermittently aerated sequencing batch reactors (IASBRs) were parallelly operated for treating digested piggery wastewater. Their microbial communities were analyzed, and the nitrogen removal performance was compared during the long–term run. IASBRs demonstrated higher removal rates of total nitrogen (TN) and ammonium nitrogen (NH4+-N) than the SBR, and also demonstrated higher resistance against TN shock load. It was found that the more switch times between aerobic/anoxic in an IASBR, the higher the removal rates of TN and NH4+–N. All the reactors were predominated by Thauera, Nitrosomonas and Nitrobacter, which were considered to be species of denitrifiers, ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB), respectively. However, the abundance and diversity was of great difference. Compared with SBR, IASBRs achieved higher abundance of denitrification–related bacteria. IASBR 1 with four aerobic/anoxic switch times was detected with 25.63% of Thauera, higher than that in IASBR 2 with two aerobic/anoxic switch times (11.57% of Thauera), and much higher than that in the SBR (only 6.19% of Thauera). IASBR 2 had the highest percentage of AOB, while IASBR 1 had the lowest percentage. The denitrifiers abundance was significantly positive correlated with the TN removal rate. However, the NH4+–N removal rate showed no significant correlation with the AOB abundance, but might relate to the AOB activity which was influenced by the average free ammonium (FA) concentration. Nitrobacter was the only NOB genus detectable in all reactors, and were less than 0.03%.
filling, switched four times between aerobic (60 min) /anoxic (40 min), settling and drawing
IASBR 2#
filling, switched twice between aerobic (120 min) /anoxic (80 min), settling and drawing
SBR
filling, switched once between aerobic (240 min) / anoxic (160 min), settling and drawing
Tab.1
Fig.1
Fig.2
reactors
OUTs
good’s coverage/%
Chao–1 index
Shannon index
ACE index
IASBR 1#
1612
96.7
3974
4.18
5766
IASBR 2#
1533
97.2
3078
4.38
4472
SBR
1157
97.6
2315
4.00
3114
Tab.2
Fig.3
Fig.4
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