Effects of nitrate concentration on biological
hydrogen production by mixed cultures

doi:10.1007/s11783-009-0142-0

Front.Environ.Sci.Eng.

2009, Vol. 3

Issue (4) : 380-386 https://doi.org/10.1007/s11783-009-0142-0

Research articles

Effects of nitrate concentration on biological hydrogen production by mixed cultures

Bo WANG,Wei WAN,Jianlong WANG,

Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China;

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Abstract The effects of nitrate on fermentative hydrogen production and soluble metabolites from mixed cultures were investigated by varying nitrate concentrations from 0 to 10g N/L at 35°C with an initial pH of 7.0. The results showed that the substrate degradation rate, hydrogen production potential, hydrogen yield, and average hydrogen production rate initially increased with increasing nitrate concentrations from 0 to 0.1g N/L, while they decreased with increasing nitrate concentrations from 0.1 to 10g N/L. The maximum hydrogen production potential of 305.0mL, maximum hydrogen yield of 313.1mL/g glucose, and maximum average hydrogen production rate of 13.3mL/h were obtained at a nitrate concentration of 0.1g N/L. The soluble metabolites produced by the mixed cultures contained only ethanol and acetic acid (HAc) without propionic acid (HPr) and butyric acid (HBu). This study used the Modified Logistic model to describe the progress of cumulative hydrogen production in batch tests. A concise model was proposed to describe the effects of nitrate concentration on average hydrogen production rate.

Keywords nitrogen source biohydrogen fermentative hydrogen production quantitative kinetic model

Issue Date: 05 December 2009

Cite this article:

Bo WANG,Jianlong WANG,Wei WAN. Effects of nitrate concentration on biological hydrogen production by mixed cultures[J]. Front.Environ.Sci.Eng., 2009, 3(4): 380-386.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-009-0142-0
https://academic.hep.com.cn/fese/EN/Y2009/V3/I4/380

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