Community dynamics of ammonia oxidizing bacteria in a full-scale wastewater treatment system with nitrification stability

doi:10.1007/s11783-010-0254-6

Front Envir Sci Eng Chin

2011, Vol. 5

Issue (1) : 92-98 https://doi.org/10.1007/s11783-010-0254-6

RESEARCH ARTICLE

Community dynamics of ammonia oxidizing bacteria in a full-scale wastewater treatment system with nitrification stability

Xiaohui WANG, Xianghua WEN(

), Hengjing YAN, Kun DING, Man HU

Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China

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Abstract

To determine whether the functional stability of nitrification was correlated to a stable community structure of ammonia oxidizing bacteria (AOB) in a full-scale wastewater treatment plant, the AOB community dynamics in a wastewater treatment system was monitored over one year. The community dynamics were investigated using specific PCR followed by terminal restriction fragment length polymorphism (T-RFLP) analysis of the amoA gene. The T-RFLP results indicated that during the period of nitrification stability, the AOB community structure in the full-scale wastewater treatment system was relatively stable, and the average change rate every 15 d of the system was 6.6%±5.8%. The phylogenetic analysis of the cloned amoA gene showed clearly that the dominant AOB in the system was Nitrosomonas spp. The results of this study indicated that throughout the study period, the AOB community structure was relatively stable in the full-scale wastewater treatment system with functional stability of nitrification.

Keywords ammonia-oxidizing bacteria (AOB) community dynamics terminal restriction fragment length polymorphism (T-RFLP) nitrification performance

Corresponding Author(s): WEN Xianghua,Email:xhwen@tsinghua.edu.cn

Issue Date: 05 March 2011

Cite this article:

Xiaohui WANG,Xianghua WEN,Hengjing YAN, et al. Community dynamics of ammonia oxidizing bacteria in a full-scale wastewater treatment system with nitrification stability[J]. Front Envir Sci Eng Chin, 2011, 5(1): 92-98.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-010-0254-6
https://academic.hep.com.cn/fese/EN/Y2011/V5/I1/92

Fig.1 Nitrification performance of the system over 345 d. (a) Ammonia concentration; (b) nitrate concentration

Fig.2 Histograms of T-RF relative abundances in the system for I T-RFLP profiles. The relative abundance is the ratio of the peak area of a given T-RF in a given sample to the sum of all T-RFs in that sample expressed as a percentage. Arrows indicate the sizes of the restriction fragments for most T-RFs in base pairs

Fig.3 Moving-window analysis based on AOB T-RFLP profiles. Each data point is the change percentage between the bacterial communities of two consecutive dates. The time span between two consecutive dates is 15 d

Fig.4 Phylogenetic tree showing the relationships of partial gene sequences to reference sequences from the GenBank database. The tree was constructed with the neighbor-joining method using 450 nucleotide positions. Clone sequences are named beginning with “A”, and T-RF sizes are given in parentheses, with “T-” indicating actual sizes determined via T-RFLP

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