Impact of dissolved oxygen on the production of nitrous oxide in biological aerated filters

doi:10.1007/s11783-017-0964-0

Front. Environ. Sci. Eng.

2017, Vol. 11

Issue (6) : 16 https://doi.org/10.1007/s11783-017-0964-0

RESEARCH ARTICLE

Impact of dissolved oxygen on the production of nitrous oxide in biological aerated filters

Qiang He, Yinying Zhu, Guo Li, Leilei Fan, Hainan Ai, Xiaoliu Huangfu, Hong Li(

)

Key Laboratory of Eco-Environment of Three Gorges Region, Ministry of Education, Chongqing University, Chongqing 400045, China

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Abstract

The dominant Cloacibacterium normanense may be responsible for N₂O production.

N₂O concentrations varied along the biofilm depth depending on the DO levels.

Low DO concentration leads to high N₂O production rate.

Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and microelectrode technology were employed to evaluate the Nitrous oxide (N₂O) production in biological aerated filters (BAFs) under varied dissolved oxygen (DO) concentrations during treating wastewater under laboratory scale. The average yield of gasous N₂O showed more than 4-fold increase when the DO levels were reduced from 6.0 to 2.0 mg·L⁻¹, indicating that low DO may drive N₂O generation. PCR-DGGE results revealed that Nitratifractor salsuginis were dominant and may be responsible for N₂O emission from the BAFs system. While at a low DO concentration (2.0 mg·L⁻¹), Flavobacterium urocaniciphilum might play a role. When DO concentration was the limiting factor (reduced from 6.0 to 2.0 mg·L⁻¹) for nitrification, it reduced NO₂⁻-N oxidation as well as the total nitrification. The data from this study contribute to explain how N₂O production changes in response to DO concentration, and may be helpful for reduction of N₂O through regulation of DO levels.

Keywords Nitrous oxide Biological aerated filter Microelectrode Dissolved oxygen Biofilm

Corresponding Author(s): Hong Li

Issue Date: 10 July 2017

Cite this article:

Qiang He,Yinying Zhu,Guo Li, et al. Impact of dissolved oxygen on the production of nitrous oxide in biological aerated filters[J]. Front. Environ. Sci. Eng., 2017, 11(6): 16.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-017-0964-0
https://academic.hep.com.cn/fese/EN/Y2017/V11/I6/16

Fig.1 Simulated BAF system in this study. a, plexiglass tank; b, flowrator (MP200-YZ15, PreFluid, Changzhou, China); c, flowrator (LZB-2, Variable-area Flowmeter, Shanghai, China); d, air pump (WT-ZWS24Z, China) and e, flowrator (Q-Flow, Zhuoang, Shanghai, China)

Fig.2 COD (a), TN (b), NH₄-N (c), NO₃^--N (d), and NO₂^--N (e) concentration in the influent and effluent of BAF systems exposed at different DO levels, pH 7.2, 20℃

Fig.3 Effect of DO on the production rate of N₂O, pH 7.2, 20℃

Tab.1 Dynamic of N₂O emission factors (N₂O /TN_influent) under varied DO levels

Fig.4 DGGE profile of 16S rRNA gene fragments of microbial communities from BAF biofilm under different DO levels, pH 7.2, 20℃

Tab.2 Results of 16S rDNA sequences using BLAST in GeneBank

Fig.5 Changes in concentrations of N₂O with depth in biofilm, pH 7.2, 20℃

Fig.6 Changes in DO concentrations (a), ORP value (b), NH₄⁺-N concentrations (c), NO₂^--N concentrations (d), NO₃^--N concentrations (e) with depth in biofilm, pH 7.2, 20℃

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