Selective targeted adsorption and inactivation of antibiotic-resistant bacteria by Cr-loaded mixed metal oxides

doi:10.1007/s11783-021-1502-7

Front. Environ. Sci. Eng.

2022, Vol. 16

Issue (6) : 68 https://doi.org/10.1007/s11783-021-1502-7

RESEARCH ARTICLE

Selective targeted adsorption and inactivation of antibiotic-resistant bacteria by Cr-loaded mixed metal oxides

Yanlin Li¹, Bo Wang^2,³(

), Lei Zhu¹, Yixing Yuan¹, Lujun Chen⁴, Jun Ma¹(

)

¹. State Key Laboratory of Urban Water Resources and Environment (SKLUWRE), Harbin Institute of Technology, Harbin 150090, China
². Tianjin Key Laboratory of Hazardous Waste Safety Disposal and Recycling Technology, School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China
³. Jiangsu Yihuan Group Co., Ltd., Yixing 214206, China
⁴. School of Environment, Tsinghua University, Beijing 100084, China

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Abstract

• LDHs and MMOs was synthesized by ultrasound-assisted one-step co-precipitation.

• MMOs performs the best for Cr(VI) and E. coli_NDM-1 simultaneous removal.

• Possible antibacterial pathways of Cr-MMOs were proposed.

Herein we provide a novel high-efficiency nanocomposite for bacterial capture based on mixed metal oxides (MMOs) with deleterious chromium properties. With both the layer structure of layered double hydroxides (LDHs) and the magnetic properties of Fe, MMOs enrich the location of ionic forms on the surface, providing a good carrier for adsorption of the heavy metal Cr(VI). The capacity for adsorption of Cr(VI) by MMOs can be as high as 98.80 mg/g. The prepared Cr(VI)-MMOs achieved extremely expeditious location of gram-negative antibiotic-resistant E. coli_NDM-1 by identifying lipid bilayers. Cr-MMOs with a Cr loading of 19.70 mg/g had the best bactericidal effect, and the concentration of E. coli_NDM-1 was decreased from ~10⁸ to ~10³ CFU/mL after 30 min of reaction. The binding of nitrogen and phosphorus hydrophilic groups to chromate generated realistic models for density functional theory (DFT) calculations. The specific selectivity of MMOs toward bacterial cells was improved by taking Cr(VI) as a transferable medium, thereby enhancing the antibacterial activity of Cr-MMOs. Under the combined action of chemical and physical reactions, Cr(VI)-MMOs achieved high capacity for inactivation of bacteria. Moreover, the metallic elements ratio in Cr-MMOs remained stable in their initial valence states after inactivation. This guaranteed high removal efficiency for both heavy metals and bacteria, allowing recycling of the adsorbent in practical applications.

Keywords Heavy metal adsorption Magnetic hydrotalcite ARBs removal Cr(VI)-MMOs combined antibacterial activity

Corresponding Author(s): Bo Wang,Jun Ma

Issue Date: 23 September 2021

Cite this article:

Yanlin Li,Bo Wang,Lei Zhu, et al. Selective targeted adsorption and inactivation of antibiotic-resistant bacteria by Cr-loaded mixed metal oxides[J]. Front. Environ. Sci. Eng., 2022, 16(6): 68.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-021-1502-7
https://academic.hep.com.cn/fese/EN/Y2022/V16/I6/68

Fig.1 Effect of contact time (a) and pH (b) on q_e (dose: 0.05 g, react time: 0–120 min, pH: 2–12, temperature: 25℃, initial concentrations: 50 mg/L); (c) Relation between adsorption dynamics and uptake of Cr(VI) on MMOs at different pH; (d) Stability of Cr-MMOs in the presence of various common inorganic compounds (dose: 0.05 g, react time: 120 min, concentrations of HNO₃, NaOH and Na₂CO₃: 0.1 mol/L, temperature: 25℃, initial concentrations: 50 mg/L).

Fig.2 (a) Influence of content of LDHs, MMOs and Cr-MMOs on E. coli_NDM-1 inactivation (E. coli_NDM-1 concentration 1 × 10⁸ CFU/mL; 120 min; 25℃); (b) Influence of time on E. coli_NDM-1 inactivation (E. coli_NDM-1 concentration 1 × 10⁸ CFU/mL; samples concentration 100 mg/L; 25℃); (c) Antibacterial activity of Cr(VI)-MMOs on E. coli_NDM-1 with different Cr(VI) dosage (E. coli_NDM-1 concentration 1 × 10⁸ CFU/mL; 30 min; 25℃); (d) Importance of different valence state of chromium in E. coli_NDM-1 removal by LDHs and MMOs (E. coli_NDM-1 concentration 1 × 10⁸ CFU/mL; 30 min; 25℃).

Fig.3 (a) Antibacterial activity of LDHs, MMOs, and Cr-MMOs at different pH; (b) Antibacterial activity of LDHs, MMOs, and Cr-MMOs in the presence of competition ions and radical inhibitor.

Fig.4 (a) Antibacterial activity of different heavy metal cations on E. coli_NDM-1; (b) Influence of morphology of Cr-MMOs on E. coli_NDM-1 removal (Process of MMOs put in hydrothermal reaction vessel with aging temperature 120℃ and aging time 12, 24, 48 and 36 h, respectively. Increasing the synthesis duration of MMOs assure high crystallinity, but the larger size and lower roughness).

Fig.5 Optimized geometric structure of amino acid-CrO₄²^- (a) and DPPC-CrO₄²^- (b) system.

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