The impact of different voltage application modes on biodegradation of chloramphenicol and shift of microbial community structure

doi:10.1007/s11783-022-1576-x

Front. Environ. Sci. Eng.

2022, Vol. 16

Issue (11) : 141 https://doi.org/10.1007/s11783-022-1576-x

RESEARCH ARTICLE

The impact of different voltage application modes on biodegradation of chloramphenicol and shift of microbial community structure

Yifan Liu^1,², Qiongfang Zhang^1,², Ainiwaer Sidike³, Nuerla Ailijiang^1,²(

), Anwar Mamat⁴, Guangxiao Zhang^1,², Miao Pu^1,², Wenhu Cheng^1,², Zhengtao Pang^1,²

¹. Key Laboratory of Oasis Ecology of Education Ministry, College of Ecology and Environment, Xinjiang University, Urumqi 830017, China
². Xinjiang Jinghe Observation and Research Station of Temperate Desert Ecosystem, Ministry of Education, Urumqi 830017, China
³. School of Geographical Sciences, Xinjiang University, Urumqi 830017, China
⁴. School of Chemical Engineering and Technology, Xinjiang University, Urumqi 830017, China

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Abstract

● Presented coupled system enhanced biodegradation of antibiotic chloramphenicol.

● HRT and electrical stimulation modes were key influencing factors.

● Electrical stimulation had little effect on the chloramphenicol metabolic pathway.

● Microbial community structure varied with the voltage application mode.

Exoelectrogenic biofilms have received considerable attention for their ability to enhance electron transfer between contaminants and electrodes in bioelectrochemical systems. In this study, we constructed anaerobic-aerobic-coupled upflow bioelectrochemical reactors (AO-UBERs) with different voltage application modes, voltages and hydraulic retention times (HRTs). In addition, we evaluated their capacity to remove chloramphenicol (CAP). AO-UBER can effectively mineralize CAP and its metabolites through electrical stimulation when an appropriate voltage is applied. The CAP removal efficiencies were ~81.1%±6.1% (intermittent voltage application mode) and 75.2%±4.6% (continuous voltage application mode) under 0.5 V supply voltage, which were ~21.5% and 15.6% greater than those in the control system without voltage applied, respectively. The removal efficiency is mainly attributed to the anaerobic chamber. High-throughput sequencing combined with catabolic pathway analysis indicated that electrical stimulation selectively enriched Megasphaera, Janthinobacterium, Pseudomonas, Emticicia, Zoogloea, Cloacibacterium and Cetobacterium, which are capable of denitrification, dechlorination and benzene ring cleavage, respectively. This study shows that under the intermittent voltage application mode, AO-UBERs are highly promising for treating antibiotic-contaminated wastewater.

Keywords Electrical stimulation Biodegradation Microbial community Chloramphenicol

Corresponding Author(s): Nuerla Ailijiang

Issue Date: 31 May 2022

Cite this article:

Yifan Liu,Qiongfang Zhang,Ainiwaer Sidike, et al. The impact of different voltage application modes on biodegradation of chloramphenicol and shift of microbial community structure[J]. Front. Environ. Sci. Eng., 2022, 16(11): 141.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-022-1576-x
https://academic.hep.com.cn/fese/EN/Y2022/V16/I11/141

Fig.1 (A) Schematic diagram of AO-UBER. (B) AO-UBER photos. (1, CFNs; 2, titanium mesh (screen mesh size of 50 mm × 50 mm); 3, titanium mesh (screen mesh size of 2 mm × 2 mm); 4, titanium plate; 5, GACs; and 6, sampling port); (R1: intermittent voltage applied; R2: continuous voltage applied; and R3: control reactor without voltage applied).

Fig.2 (A) CAP removal performances of AO-UBERs during the long-term operation and (B) COD removal performances of AO-UBERs during stage 5.

Fig.3 CAP removal performance in the anaerobic zones (A) and aerobic zones (B) of AO-UBERs under different HRTs and applied voltages.

Tab.1 Microbial community diversity, abundance index and Q-PCR quantification of microbial samples

Fig.4 Relative abundance of bacterial community composition in (A) eight samples at the phylum level and (B) the heatmap of the most abundant genera. Only the most populous 41 genera were used to build the heat map, and the color intensity showed the relative abundance of the genera in a sample. RAn and RAe were from anaerobic and aerobic seed sludges. IAn, CAn and ZAn biofilm samples were from the anaerobic zones of R1, R2 and R3; IAe, CAe and ZAe biofilm samples from the aerobic zones of R1, R2 and R3.

Fig.5 Metabolic pathway of chloramphenicol and its metabolites in the reactor.

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