Molecular detection and drought resistance analysis
of B-transgenic poplars ( × )

doi:10.1007/s11461-008-0029-4

Front. For. China

2008, Vol. 3

Issue (2) : 226-231 https://doi.org/10.1007/s11461-008-0029-4

Molecular detection and drought resistance analysis of B-transgenic poplars ( × )

LI Yiliang¹, SU Xiaohua², ZHANG Bingyu², ZHANG Zhiyi³

1.Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University；Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry; 2.Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry; 3.Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University

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Abstract The propagation of drought-resistant transgenic poplars is one of the more effective ways to improve the ecological state of arid regions. The expression of SacB gene in transgenic lines of Populus alba × P. glandulosa was analyzed by a semi-quantitative RT-PCR. Drought resistance of these transgenic lines was evaluated by water stress trials in a greenhouse. Results showed that the mRNA transcription of SacB was found in all transgenic lines and fructan has accumulated in the leaves of these plants. The growth, biomass and leaf water content of some transgenic lines were significantly higher than those of the control plants under drought stress. Correlation analysis indicated that the said factors were significantly and positively related to the concentration of fructan in the leaves of the transgenic lines. Results revealed that the expression of SacB gene in transgenic poplars enhanced its resistance to water stress.

Issue Date: 05 June 2008

Cite this article:

SU Xiaohua,LI Yiliang,ZHANG Bingyu, et al. Molecular detection and drought resistance analysis of B-transgenic poplars ( × )[J]. Front. For. China, 2008, 3(2): 226-231.

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https://academic.hep.com.cn/ffc/EN/10.1007/s11461-008-0029-4
https://academic.hep.com.cn/ffc/EN/Y2008/V3/I2/226

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