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Frontiers of Forestry in China

ISSN 1673-3517

ISSN 1673-3630(Online)

CN 11-5728/S

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Front Fore Chin    2009, Vol. 4 Issue (1) : 96-100    https://doi.org/10.1007/s11461-009-0005-7
RESEARCH ARTICLE
Genetic diversity of Quercus glandulifera var. brevipetiolata populations in three forest communities with different succession stages
Junmin LI1(), Zexin JIN1, Qiping GU2, Wenyan LOU3
1. Institute of Ecology, Taizhou University, Linhai 317000, China; 2. Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Faculty of Life Science, Southwest China University, Chongqing 400715, China; 3. Yiting Middle School, Yiwu 322005, China
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Abstract

In order to understand the relationship between population succession and its genetic behavior, random amplified polymorphic DNA (RAPD) technique was used to analyze the genetic diversity of Quercu glandulifera var. brevipetiolata populations in three forest communities with different succession stages (coniferous forest, coniferous and broad-leaved mixed forest, evergreen broad-leaved forest). The results showed that 145 repetitive loci were produced in 60 individuals of Q. glandulifera using 11 primers, among which 125 loci were polymorphic, and the total percentage of polymorphic loci was 82.76% with an average of 64.14%. Estimated by the Shannon information index, the total genetic diversity of the three populations was 0.4747, with an average of 0.3642, while it was 0.3587, with an average of 0.3265, judged from the Nei index. Judged from percentage of polymorphic loci, Shannon inform at ion index and Nei index, the genetic diversity followed a decreasing order: coniferous forest>broad-leaved mixed forest>evergreen broad-leaved forest. Analysis of molecular variance (AMOVA) showed that 69.73% of the genetic variance existed within populations and 20.27% of the genetic variance existed among populations. The coefficient of gene differentiation (GST) was 0.2319 and the gene flow (Nm) was 1.6539. The mean of genetic identity among populations of Q. glandulifera was 0.8501 and the mean of genetic distance was 0.1626. The genetic identity between the Q. glandulifera population in the coniferous forest and that in the coniferous and broad-leaved mixed forest was the highest. UPGMA cluster analysis based on Nei’s genetic distance showed that the population in the coniferous forest gathered with that in the coniferous and broad-leaved mixed forest firstly, then with that in the evergreen broad-leaved forest. The genetic structure of Q. glandulifera was not only characteristic of the biological characteristics of this species, but was also influenced by the microenvironment in different communities.
Keywords Quercus glandulifera var. brevipetiolata      random amplified polymorphic DNA RAPD      genetic diversity      succession     
Corresponding Author(s): LI Junmin,Email:lijm@tzc.edu.cn   
Issue Date: 05 March 2009
 Cite this article:   
Junmin LI,Zexin JIN,Qiping GU, et al. Genetic diversity of Quercus glandulifera var. brevipetiolata populations in three forest communities with different succession stages[J]. Front Fore Chin, 2009, 4(1): 96-100.
 URL:  
https://academic.hep.com.cn/ffc/EN/10.1007/s11461-009-0005-7
https://academic.hep.com.cn/ffc/EN/Y2009/V4/I1/96
primerssequences (5￠-3￠)
S370GTGCAACGTG
S335CAGGGCTTTC
S112ACGCGCATGT
S333GACTAAGCCC
S327CCAGGAGGAC
S306ACGCCAGAGG
S69CTCACCGTCC
S94GGATGAGACC
S309GGTCTGGTTG
S81CTACGGAGGA
S380GTGTCGCGAG
Tab.1  Sequences of 11 random primers used in the RAPD analysis
populationsD1D2D3averagetotal
number of samples2020202060
total number of loci145145145145145
number of polymorphic loci97948893120
percentage of polymorphic loci/%66.9064.8360.6964.1482.76
Shannon information index0.38720.35960.34590.36420.4747
Nei index0.26500.24360.23650.24840.3234
Tab.2  Genetic diversity of the three populations of
Shannon information indexNei index
genetic diversity within population, Hpop0.3642gene diversity within population, Hs0.2484
total genetic diversity, Hsp0.4747total gene diversity, Ht0.3234
ratio of genetic diversity within population, Hpop/Hsp0.7672ratio of gene diversity within population, Hs/Ht0.7681
ratio of genetic diversity among populations, (Hsp-Hpop)/Hsp0.2328the coefficient of gene differentiation, GST0.2319
gene flow, Nm1.6539
Tab.3  Partitioning of the genetic diversity within and among 3 populations of
source of variationdegree of freedomsum of squared deviationsmean squared deviationsvariance componentpercentage of total variance/%p-value
among populations2282.2333141.11706.326930.27<0.001
within populations57830.950014.578014.587169.73<0.001
Tab.4  Analysis of molecular variance (AMOVA) within and among populations of
populationD1D2D3
D1–0.84350.8310
D20.1702–0.8758
D30.18510.1326–
Tab.5  Genetic identity and genetic distance among the three populations of
Fig.1  Cluster figure produced by POPGENE32 soft for the three populations of
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