Role of cell wall degrading enzymes in the interaction of poplar and <italic>Melampsora larici-populina </italic>Kleb.

doi:10.1007/s11461-009-0006-6

Front Fore Chin

2009, Vol. 4

Issue (1) : 111-116 https://doi.org/10.1007/s11461-009-0006-6

RESEARCH ARTICLE

Role of cell wall degrading enzymes in the interaction of poplar and Melampsora larici-populina Kleb.

Chengming TIAN¹(

), Peng ZHAO², Zhimin CAO²

1. The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China; 2. College of Forestry, Northwest A & F University, Yangling 712100, China

Download: PDF(174 KB) HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

Abstract

The activity of cell wall-degrading enzymes, produced in poplar cultivars infected Melampsora larici-populina Kleb., was studied. The results show that PMG, PMTE, C_X and β-glucosidase played roles during the infection. After inoculation, the activity of PMG in both susceptible and resistant cultivars had two peak values in 2 dpi and 5 dpi. The activities of PMTE and β-glucosidase had a peak value in 3 dpi, and C_X in 2 dpi. Among these cell wall-degrading enzymes, the activities of PMG and PMTE were higher and the activities of C_X and β-glucosidase were relatively lower. The activities of these cell wall-degrading enzymes were significantly higher in susceptible cultivars than those in resistant cultivars. All these demonstrated that these cell wall-degrading enzymes played certain roles in the infection of M. larici-populina.

Keywords Melampsora larici-populina cell wall-degrading enzymes poplar

Corresponding Author(s): TIAN Chengming,Email:chengmt@bjfu.edu.cn

Issue Date: 05 March 2009

Cite this article:

Peng ZHAO,Zhimin CAO,Chengming TIAN. Role of cell wall degrading enzymes in the interaction of poplar and Melampsora larici-populina Kleb.[J]. Front Fore Chin, 2009, 4(1): 111-116.

URL:

https://academic.hep.com.cn/ffc/EN/10.1007/s11461-009-0006-6
https://academic.hep.com.cn/ffc/EN/Y2009/V4/I1/111

Fig.1 Changes of PMG activity in infected by

Fig.2 Changes of PMG activity in infected by

Fig.3 Changes of PMTE activity in infected by

Fig.4 Changes of PMTE activity in infected by

Fig.5 Changes of C activity in infected by

Fig.6 Changes of C activity in infected by

Fig.7 Changes of -G activity in infected by

Fig.8 Changes of -G activity in infected

1	Cao Z M, Li Z Q, Hu J J (1998). Physiological specialization in Melampsora larici-populinaKleb. in the Qinling Mountains . J Northwest For Coll,13(1): 53–57 (in Chinese)
2	Chen X J, Zhang H, Xu J Y, Tong Y H, Ji Z L (2006). Cell wall degrading enzymes produced by Rhizoctonia solaniand their pathogenicity to rice plants. Jiangsu J Agr Sci , 22(1): 24–28 (in Chinese)
3	Deising H, Frittrang A K, Kunz S, Mendgen K ,(1995). Regulation of pectin methylesterase and polygalacturonate lyase activity during differentiation of infection structures in Uromyces viciae-fabae. Microbiology , 141:561–571
4	Fan Z H, Shen R X, Zhou Z M. (1989). The relationships between the activities of peroxidase and phynelalanine ammonialyase and the resistance of poplar to leaf rust. Acta Phytopathol Sin , 19(2): 95–100 (in Chinese)
5	Feng J, Gao Z G, Xue C S, Zhuang J H, Chen J, Bai S Y (2002). The pathogenesis of the cell-degrading enzymes produced by Curvularia lunata. Rain Fed Crop , 22(3): 164–166 (in Chinese)
6	Heiler S, Mendgen K, Deising H (1993). Cellulotic enzymes of the obligately biotrophic rust fungus Uromyces viciae-fabaeare regulated differentiation-specifically. Mycol Res , 97: 77–85
7	Larson P R, Isebrands J G. (1971). The plastochron index as applied to developmental studies of cottonwood. Can J For Res , 1: 1–11 doi: 10.1139/x71-001
8	Li B J, Chen L Q, Meng W J, Wang F J (2003). Effect of temperature and humidity on cell wall degrading enzymes produced by Botrytis cinereaduring infection of tomato plant. Acta Phytopathol Sin , 33(3): 209–212 (in Chinese)
9	Li B J, Zhou C L, Zhao K H, Li F Y (2000). Pathogenic mechanism of scab of cucumber caused by Cladosporium cucumerinum II: The cell wall degrading enzymes and its pathogenic action. Acta Phytopathol Sin , 30(1): 13–18 (in Chinese)
10	Lu X F (2005). Study on Proteins in Interaction System between Wheat and Stripe Rust Fungi. Yangling: Northwest A & F University (in Chinese)
11	Pei M H, McCracken A R (2005). Rust Diseases of Willow and Poplar. CABI Publishing , 1–264
12	Tian C M, Li Z Q, Kang Z S (1999). Advances in researches of cathay poplar leaf rust (Melampsora larici populina Kleb.). J Northwest For Coll , 14(2): 81–88 (in Chinese)
13	Tian C M, Liang Y M, Kang Z S, Li Z Q, Zhao Y X (2002). Ultrastructure of poplar leaf Infected by rust fungus (Melampsora larici-populina Kleb.). Acta Phytopathol Sin , 32(1): 71–78 (in Chinese)
14	Wei Y N (1984). A study on activities of polyphenol oxidase and peroxidase, and tendency in changes of isozyme spectra in Chinese white poplar (Populus tomentosa Carr.) leaves after infected with Melompsora magnusianaWagner. J Beijing For Univ , 17(3): 73–92 (in Chinese)
15	Xu H, Mendgen K (1997). Targeted cell wall degradation at the penetration site of cowpea rust basidiosporelings. Am Phytopathol Soc , 10(1): 87–94
16	Zhao L, Zhang T Y (2002). The role of degrading enzymes in plant-pathogen interaction. Microbiology , 29(1): 89–93 (in Chinese)

[1]	Lei MA, Xiaoqin WU, Ling ZHENG. Relationship between plant hormone level excreted by ectomycorrhizal fungi and growth of poplar NL-895[J]. Front Fore Chin, 2009, 4(2): 236-241.
[2]	MA Lei, WU Xiaoqin, ZHENG Ling. Mycorrhizal formation of nine ectomycorrhizal fungi on poplar cuttings[J]. Front. For. China, 2008, 3(4): 475-479.
[3]	WEI Yankui, JIA Liming, WANG Ling, QIU Guoqing, XING Changshan. Characteristics of root growth in a fast-growing and high-yield poplar plantation under subsurface drip-irrigation[J]. Front. For. China, 2008, 3(1): 98-105.
[4]	LAN Shibo, LUO Xu, LUO Yuliang. The growth process of natural poplar-birch forests[J]. Front. For. China, 2006, 1(3): 277-284.
[5]	Yuan Shengliang, Gao Baojia, Zhang Na. Insect-resistant mechanism of transgenic triploid of Chinese white poplar[J]. Front. For. China, 2006, 1(2): 196-200.
[6]	Fang Shengzuo, Tian Ye, Yuan Fayin. Effects of Cutting Density on Growth, Yield and Quality of Poplar Clone Seedlings[J]. Front. For. China, 2006, 1(1): 64-69.
[7]	Zhai Mingpu, Jiang Sannai, Jia Liming. Nutrient Dynamics of Fine Roots in the Mixed Plantation of Poplar and Black Locust[J]. Front. For. China, 2006, 1(1): 70-75.

Viewed

Full text

Abstract

Cited

Shared

Discussed