Effect of NaCl stress on ion distribution in roots and growth of <italic>Cyclocarya paliurus</italic> seedlings

doi:10.1007/s11461-009-0007-5

Front Fore Chin

2009, Vol. 4

Issue (2) : 208-215 https://doi.org/10.1007/s11461-009-0007-5

RESEARCH ARTICLE

Effect of NaCl stress on ion distribution in roots and growth of Cyclocarya paliurus seedlings

Ruiling YAO, Shengzuo FANG(

)

College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, china

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Abstract

We studied ion distribution in roots and the growth of Cyclocarya paliurus seedlings of three provenances, Huangshan in Anhui, Jiujiang in Jiangxi and Kunming in Yunnan, under conditions of 0, 1, 3 and 5 g/L NaCl stress using X-ray microanalysis. Results show that under NaCl stress of 3 and 5 g/L, the relative contents of Na⁺ and Cl^– in root tissues increased, while the relative content of K⁺, Ca²⁺ and Mg²⁺ decreased. With an increase in salinity, the relative contents of Na⁺ in the epidermis and cortex of the root increased, while the relative content of Cl^– in the stele and cortex of the root increased markedly. Thus, ions in the root tissues were unbalanced and the ratios K⁺/Na⁺ and Ca²⁺/Na⁺ decreased, while Na⁺/(K⁺+Na⁺+Ca²⁺+Mg²⁺) increased. The decrease of the K⁺/Na⁺ ratio and the substantial increase of Cl^– in root tissues contributed to a decline in seedlings survival and reduced the increments for seedling leaf area, height, basal diameter as well biomass. Our preliminary conclusion is that the level of salt tolerance for the tested provenance seedlings was in the order of Huangshan>Kunming>Jiujiang, and the threshold of salt tolerance for C. paliurus seedlings was about 1 g/L.

Keywords NaCl stress Cyclocarya paliurus ion distribution root tissue X-ray microanalysis

Corresponding Author(s): FANG Shengzuo,Email:fangsz@njfu.com.cn

Issue Date: 05 June 2009

Cite this article:

Shengzuo FANG,Ruiling YAO. Effect of NaCl stress on ion distribution in roots and growth of Cyclocarya paliurus seedlings[J]. Front Fore Chin, 2009, 4(2): 208-215.

URL:

https://academic.hep.com.cn/ffc/EN/10.1007/s11461-009-0007-5
https://academic.hep.com.cn/ffc/EN/Y2009/V4/I2/208

Fig.1 Ion distribution in the root tissues after 23 days of salt stress
Note: H, J, K show Huangshan, Jiujiang, Kunming provenances, while CK, S, S, S show 0, 17.0, 51.0 and 85.0 mol/L NaCl treatments, respectively.

Tab.1 Ion ratios in root tissues after 23 days of salt stress

Tab.2 Ion ratios of cortex to epidermis and stele to cortex in roots after 23 days of salt stress

Fig.2 Survival percentage of seedlings under salt stress

Tab.3 Analysis of variance of seedling growth after 28 days of salt stress

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