Investigation of anti-salt stress on tetraploid <italic>Robinia pseudoacacia

doi:10.1007/s11461-009-0023-5

Front Fore Chin

2009, Vol. 4

Issue (2) : 227-235 https://doi.org/10.1007/s11461-009-0023-5

RESEARCH ARTICLE

Investigation of anti-salt stress on tetraploid Robinia pseudoacacia

Fanjuan MENG¹, Jiangjiang WANG¹, Chuanping YANG¹(

), Jianxin LIU²

1. College of Life Science, Northeast Forestry University, Harbin 150040, China; 2. Biotechnological Center of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

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Abstract

Tetraploid Robinia pseudoacacia was used as a main test material and diploid R. pseudoacacia was used as the control. The indices of shape, physiology and biochemistry, photosynthesis and anatomic structure of the young plants were investigated under salt stress (NaCl and Na₂SO₄). The treatment time was 30 d with an interval time of 7 d. Before and after treatment, the indices were measured. Results show that: 1) the growth of diploid R. pseudoacacia inhibited an evident symptom of salt damage and the leaf moisture content was lower under salt stress than that of control. But the tetraploid R. pseudoacacia was contrary. 2) The relative electric conductivity and proline (Pro) of tetraploid R. pseudoacacia increased slightly and had no significant difference compared with its control, which was contrary to diploid R. pseudoacacia. At the same time, three protective enzymes including perocidase (POD), superoxide (SOD) and catalase (CAT) kept higher activities at a post stage of salt stress to tetraploid R. pseudoacacia, which enhanced its anti-salt characteristics. Diploid R. pseudoacacia was sensitive to salt and had contrary information. 3) Salt stress had little influence to photosynthesis of tetraploid R. pseudoacacia. The net photosynthetic rate (P_n) and intercellular CO₂ concentration (C_i) had no significant changes, but those of diploid R. pseudoacacia decreased singificantly. 4) After salt stress, the anatomic structure of tetraploid R. pseudoacacia had a positive reaction, including the palisade parenchyma of diachyma, was prolonged and arranged more tightly. The spongy parenchyma was shrunk and was arranged tightly, which was contrary with diploid R. pseudoacacia. These data demonstrate that tetraploid R. pseudoacacia had superior anti-salt performance.

Keywords tetraploid Robinia pseudoacacia diploid Robinia pseudoacacia salt stress salt resistance

Corresponding Author(s): YANG Chuanping,Email:yangcp@mail.nefu.edu.cn

Issue Date: 05 June 2009

Cite this article:

Fanjuan MENG,Jiangjiang WANG,Chuanping YANG, et al. Investigation of anti-salt stress on tetraploid Robinia pseudoacacia[J]. Front Fore Chin, 2009, 4(2): 227-235.

URL:

https://academic.hep.com.cn/ffc/EN/10.1007/s11461-009-0023-5
https://academic.hep.com.cn/ffc/EN/Y2009/V4/I2/227

Fig.1 Effects of salt stress on increasing amplitude of plant shape indices

Tab.1 Comparison of tatal chlorophyll content, FW, DW and MC of leaves under salt stress

Fig.2 Effects of salt stress on REC and Pro content of leaves

Fig.3 Effects of POD, SOD, CAT activities on leaves under salt stress

Fig.4 Effects of , , and under salt stress

Fig.5 Anatomic structure of leaf on CK1 (a), CK2 (b), EL (d), ES (c), SL (e), SS (f) (20×)

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