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Phylogenetic relationship of genus Lespedeza by ITS sequence data |
| Xiurong WANG1,Yang ZHAO1,Ruiqi PIAN2,Xiaoyang CHEN3, |
| 1.Forestry College of
Guizhou University, Guiyang 550225, China; 2.College of Biological
Sciences and Biotechnology, Beijing Forestry University, Beijing 100083,
China; 3.South China Agricultural
University, Guangzhou 510642, China; |
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Abstract ITS sequences of ten kinds of plants of Lespedeza and an out group were obtained by primer design PCR, sequencing and cluster analysis. The results show that ITS1 section length was 228―243 bp, 5.8S sequence length was 165 bp which was very conservative; ITS2 section lengths varied from 215 to 220 bp and the conservative sites occupied 88.1%. Phylogenetic analysis of ITS sequences using PAUP 4.0 software and their genetic relationship were discussed.
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| Keywords
Lespedeza Michx
internal transcribed spacer (ITS)
phylogeny
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Issue Date: 05 December 2009
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